本文關鍵詞：不同磷條件下高溫脅迫對銅綠微囊藻生長的影響及恢復研究　出處：《南京信息工程大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 高溫脅迫 磷濃度 銅綠微囊藻 超補償 恢復生長

【摘要】：在藍藻水華多發(fā)的夏季,水體溫度和區(qū)域氣溫往往在30～40℃,并且連續(xù)數(shù)日的高溫天氣均有出現(xiàn)。通過對藻類補償作用進行研究,了解其在脅迫條件下的生長及恢復規(guī)律對于探索藍藻暴發(fā)條件具有重要的生態(tài)意義,對于城市湖泊和水庫有重要的應用價值。本文以銅綠微囊藻(Microcystis aeruginosa)為材料,研究了低磷(0.05mg·L-1)、中磷(0.50mg·L-1)和高磷(500mg·L-1)三種條件下高溫脅迫下的生長和恢復狀況。以25℃為對照,設置35℃和40℃高溫,分別處理3d、6d和12d后轉入正常溫度培養(yǎng)30d,測定生長過程中的細胞密度、色素含量、丙二醛和酶活性。主要研究結論如下：(1)高溫脅迫階段,不同磷條件下銅綠微囊藻的生長狀況不同。35℃高溫脅迫下,隨磷濃度的增加,高溫對細胞增殖的抑制作用增強,低磷、中磷和高磷三種條件下,脅迫第12d時,葉綠素a含量分別比對照組下降了29.03%、24.49%和22.27%,類胡蘿卜素合成并未受到顯著抑制：抗氧化酶活性升高,脅迫時間越長,丙二醛含量越高。40℃高溫脅迫下,細胞密度和色素合成均受到顯著抑制,脅迫時間越長,細胞受損越嚴重。(2)恢復培養(yǎng)階段,不同磷條件下銅綠微囊藻的恢復生長狀況與補償效果不同。35℃脅迫解除后,低磷條件下,6d和12d組的細胞密度呈現(xiàn)超補償,30d平均補償指數(shù)Gm/Cm分別為1.27和1.20；中磷條件下,6d和12d組的細胞密度呈現(xiàn)超補償,各組葉綠素a含量均呈現(xiàn)超補償,其中6d組最為突出；高磷條件下,3d和6d組的色素含量呈現(xiàn)超補償。40℃脅迫解除后,中磷條件下,6d組的細胞密度和葉綠素a呈現(xiàn)超補償；高磷條件下,3d組的細胞密度和類胡蘿卜素呈現(xiàn)超補償,脅迫時間越長,補償效果越差。(3)銅綠微囊藻細胞的恢復狀況與抗氧化系統(tǒng)的抵御和恢復能力有密切關系。35℃脅迫時間越長,恢復培養(yǎng)階段丙二醛含量越高,適宜的磷營養(yǎng)有利于細胞膜功能的恢復。低磷和中磷條件下,35℃脅迫時間越長,酶活性越高；高磷條件下,6d組酶活性較高。40℃脅迫解除后,低磷條件下丙二醛含量和酶活性總體表現(xiàn)為6d組12d組3d組；中磷和高磷條件下,脅迫時間越長,丙二醛含量越高,脅迫時間較短(3d)有利于抗氧化系統(tǒng)的恢復。(4)通過Logistic模型,對銅綠微囊藻細胞的生長曲線進行了模擬和參數(shù)分析。35℃處理組表現(xiàn)為磷濃度越高,環(huán)境容納量越大,tp越晚,r越低；40℃處理組表現(xiàn)為磷濃度越高,細胞密度越大,環(huán)境容納量越大。對于35℃高溫脅迫,低磷條件下處理6d、中磷條件下處理12d和高磷條件下處理6d,會引起超補償效應,而高磷條件下處理3d會導致更大程度的超補償。對于40℃高溫脅迫,中磷條件下處理6d、高磷條件下處理3d和6d,會引起超補償效應,6d組預計39d后超過3d組,在更長時間上達到更多的細胞數(shù)目。高溫誘發(fā)超補償生長與自然界中藻類暴發(fā)的過程存在相似性,這種生物內(nèi)源性因素及藻體生理特征的變化進一步為湖泊水華發(fā)生的理論研究提供依據(jù)。
[Abstract]:Multiple algal blooms in the summer, the water temperature and air temperature in the region often 30 to 40 DEG C, and the number of consecutive days of high temperature weather have appeared. Through the study of algae compensation, understand its growth under stress conditions and restoration of blue-green algae to explore conditions has important ecological significance, has important application value for the city of lakes and reservoirs. In this paper, Microcystis aeruginosa (Microcystis aeruginosa) as material, the effect of low phosphorus (0.05mg, L-1), P (0.50mg - L-1) and high phosphorus (500mg, L-1) under the three conditions under high temperature stress on growth and recovery. By 25 DEG C as control, set the 35 degrees and 40 degrees high temperature, respectively 3D, 6D and 12D then transferred to normal temperature 30d culture, growth process in the determination of cell density, pigment content, MDA content and enzyme activity. The main conclusions are as follows: (1) Gao Wenxie forced stage, under the condition of different phosphorus copper The growth status of green Microcystis in different high temperature.35 degrees of stress, with the increase of phosphorus concentration, inhibition effect of high temperature on cell proliferation enhancement, low phosphorus, phosphorus and high phosphorus under the three conditions, the 12D stress, the content of chlorophyll a were higher than the control group decreased by 29.03%, 24.49% and 22.27%, carotenoid the synthesis did not significantly inhibited the increased antioxidant enzyme activity, the longer the duration of stress, MDA content is higher.40 C under high temperature stress, the density of cells and pigment synthesis was inhibited significantly, the stress time was longer, the more serious of the damaged cells. (2) Hui Fupei raising stage, different phosphorus conditions of Microcystis aeruginosa growth recovery effect the status and compensation of different.35 C after stress relief, low phosphorus conditions, 6D and 12D. The cell density showed over compensation, 30d average compensation index of Gm/Cm were 1.27 and 1.20; in phosphorus conditions, 6D and 12D. The cell density showed over compensation, The content of chlorophyll a showed over compensation, which 6D group is most prominent; high phosphorus conditions, the pigment content of 3D and 6D groups showed compensatory.40 C releasing stress, phosphorus conditions, cell density and chlorophyll 6D group A showed overcompensation; high phosphorus conditions, cells in the 3D group. And carotenoid showed over compensation, the stress time was longer, the compensation effect is worse. (3) recovery and antioxidant system of Microcystis aeruginosa resistance and recovery capability is closely related to.35 DEG C stress time longer, recovery training stage MDA content is high, suitable for phosphorus nutrition is conducive to cell membrane function the recovery of low phosphorus and phosphorus. Under the condition of 35 DEG C, the stress time was longer, the higher the enzyme activity; high phosphorus conditions, high 6D enzymatic activity of.40 group C after removing stress under low P MDA content and enzyme activity in the overall performance of 6D group 12D group 3D group; middle and high phosphorus Under the condition of stress time, the higher the content of MDA, stress time shorter (3D) to the antioxidant system recovery. (4) through the Logistic model, the growth curve of Microcystis cells were simulated and parameters analysis of.35 treatment group showed higher phosphorus concentration, the greater the carrying capacity of the environment TP, later, the lower R; 40 treatment group showed higher phosphorus concentration, cell density, environmental capacity is greater. For the 35 high temperature stress, 6D treatment of low phosphorus conditions, 12D treatment and high phosphorus conditions in 6D phosphorus conditions, will cause the super compensation effect. While the high phosphorus conditions 3D can lead to excess compensation to a greater extent. For the 40 high temperature stress, 6D treatment in phosphorus conditions, 3D and 6D for treatment of high phosphorus conditions, will cause the super compensation effect, 6D group is expected after 39d than that in group 3D, the number of cells reached more in the longer time high. Induced compensatory growth It is similar to the process of algal blooms in nature. The variation of biological endogenous factors and physiological characteristics of algae will further provide theoretical basis for the theoretical research of Lake blooms.

【學位授予單位】：南京信息工程大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X52;X173

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