本文關鍵詞：兩種碳源條件下好氧顆粒污泥中細菌的群落結構研究　出處：《武漢理工大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 碳源 好氧顆粒污泥 菌群結構 脫氮除磷

【摘要】：好氧顆粒污泥具有沉降性能優(yōu)異、微生物濃度較高等特點,在污水脫氮除磷方面具有良好的應用潛力。已有研究表明,碳源的組成對顆粒污泥的脫氮除磷性能具有重要影響,但目前關于碳源對顆粒污泥影響的研究主要集中在對污泥理化性質和內(nèi)部結構的影響方面,缺少對不同碳源條件下顆粒污泥中細菌,尤其是功能菌組成的系統(tǒng)研究。本文通過構建16S rDNA文庫,研究了分別以葡萄糖、黃水作為碳源的兩個序批式反應器(SBR)中好氧顆粒污泥的菌群結構,并對不同碳源條件下顆粒污泥中細菌的多樣性和功能菌進行了比較和分析,主要研究內(nèi)容及結果如下:(1)分別構建了兩種碳源條件下好氧顆粒污泥的16S rDNA文庫,均得到155個陽性克隆子。酶切分型結果表明,2個文庫可分別劃分為53個(葡萄糖文庫)和90個(黃水文庫)可分類操作單元(OTU)。葡萄糖文庫的物種豐富度指數(shù)Schao1為89.2,黃水文庫的為183.2。黃水培養(yǎng)的顆粒污泥中細菌的多樣性遠高于葡萄糖培養(yǎng)的顆粒污泥,說明進水基質對顆粒污泥中細菌的多樣性有顯著影響。(2)對兩文庫中克隆子數(shù)大于1的OTU進行了測序,分別獲得26條(葡萄糖文庫)和30條(黃水文庫)16S rDNA序列。序列分析結果表明,黃水文庫中已測序的細菌分別來自Proteobacteria、Acidobacteria、Chloroflexi和Bacteroidetes 4個門,而葡萄糖文庫中除了這4個門的菌以外還檢測到了Candidatus Saccharibacteria門和Planctomycetes門的菌。(3)從屬的水平上講,2個文庫的菌群組成有很大的差異。葡萄糖文庫中只有一個能鑒定到屬的優(yōu)勢類群(Candidatus Accumulibacter菌),該類群占已測序克隆的23.4%;其它優(yōu)勢類群均與已知菌屬同源性較低,它們均占已測序克隆數(shù)的5.5%~8.6%。黃水文庫的優(yōu)勢菌為Thauera、Dechloromonas、Nitrosomonas和Hydrogenophaga,分別占已測序克隆數(shù)的16.8%、8.4%、8.4%和5.3%;此外,還有少量與已知菌屬同源性較低的菌(占已測序克隆的5.3%~8.4%)。2個文庫優(yōu)勢菌的組成差異顯著,說明碳源對顆粒污泥的菌群結構有重要影響。(4)葡萄糖文庫和黃水文庫中均檢測到了與除磷和反硝化有關的細菌。2個文庫中的聚磷菌均為Candidatus Accumulibacter菌,但葡萄糖文庫中該菌所占的比例(23.4%)高于黃水文庫(8.4%)。葡萄糖文庫中只檢測到一種反硝化菌(Denitratisoma-like菌),而黃水文庫中與反硝化有關的菌包括Thauera、Dechloromonas、Zoogloea以及和1個與Sulfuritalea親緣關系較近的細菌類群。此外,黃水文庫中反硝化菌在已測序克隆中所占的比例(29.5%)高于葡萄糖文庫(7.0%)。這些差異可能是由于2個反應器N、P負荷不同引起的。(5)黃水文庫中檢測到了可能具有降解芳香族化合物功能、氨氧化功能或能夠分泌胞外多聚物(EPS)的菌,而在葡萄糖文庫中沒有檢測到具有這些功能的菌,說明黃水培養(yǎng)的顆粒污泥細菌的功能多樣性更豐富,更適合用來處理成分復雜的有機廢水。
[Abstract]:Aerobic granular sludge has the characteristics of excellent settling property and high microbial concentration, and has good application potential in the aspect of nitrogen and phosphorus removal in sewage. Studies have shown that the composition of carbon source has important influence on the performance of nitrogen and phosphorus removal of granular sludge, but the current research on the effects of carbon source on the granular sludge mainly concentrated on the influence of physicochemical properties and internal structure of sludge, lack of bacteria in granular sludge under different carbon source conditions, especially the system of functional bacteria. In this paper, by constructing the 16S rDNA library, was studied by glucose, water was used as carbon source two sequencing batch reactor (SBR) flora in aerobic granular sludge, and the diversity and function of bacteria granular sludge with different carbon source conditions were compared and analyzed. The main research contents and the results are as follows: (1) were constructed with 16S rDNA Library of aerobic granular sludge under the condition of two kinds of carbon sources, obtained 155 positive clones. RFLP results show that 2 libraries can be divided into 53 (glucose Library) and 90 (yellow Library) classification operation unit (OTU). The species richness index of Schao1 glucose was up to 89.2, water was up to 183.2. The water culture of the diversity of bacteria in granular sludge is much higher than that of granular sludge on glucose, and that water matrix on bacterial diversity in granular sludge had significant effect. (2) of two library clones number is greater than 1 OTU were sequenced, respectively (26 glucose Library) and 30 (yellow Library) 16S rDNA sequence. The results of sequence analysis showed that the bacteria in the water have been sequencing library respectively from Proteobacteria, Acidobacteria, Chloroflexi and Bacteroidetes 4, while glucose library besides these 4 door bacteria were detected Candidatus Saccharibacteria and Planctomycetes gates of bacteria. (3) from the level of the subordinate, there is a great difference in the composition of the bacteria in the 2 library. Only one of the glucose libraries can identify the dominant group (Candidatus Accumulibacter), which accounts for 23.4% of the cloned clones, and the other dominant groups are low homology with the known bacteria. They all account for 5.5%~8.6% of the number of sequenced clones. The dominant bacteria yellow library was Thauera and Dechloromonas, Nitrosomonas and Hydrogenophaga, respectively, accounted for 16.8% of the number of sequenced clones, 8.4%, 8.4% and 5.3%; in addition, there are a small number of known bacteria with low homology bacteria (representing sequencing cloned 5.3%~8.4%). There were significant differences in the composition of the dominant bacteria in the 2 libraries, indicating that the carbon source had an important influence on the structure of the bacterial flora of the granular sludge. (4) glucose library and library water were detected with phosphorus removal and denitrification of bacteria. PAOs 2 libraries were Candidatus Accumulibacter bacteria, but bacteria accounted for the proportion of the glucose Library (23.4%) is higher than that of yellow Library (8.4%). Glucose library only detected a denitrifying bacteria (Denitratisoma-like bacteria), and yellow library and denitrifying bacteria including related bacterial taxa near Thauera, Dechloromonas, Zoogloea, and 1 Sulfuritalea and genetic relationship. In addition, denitrifying bacteria yellow library occupied in the sequenced clones in the library (29.5%) is higher than the proportion of glucose (7%). These differences may be caused by the different loads of the 2 reactors, N and P. (5) was detected in the library may have aromatic compound degrading function, ammonia oxidation function or secretion of extracellular polymeric substances (EPS) of the bacteria, but not in the glucose library were detected with these features that water cultured bacteria, granular sludge bacterial functional diversity is more abundant, more suitable for the treatment of organic wastewater by complex components.
【學位授予單位】：武漢理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X703;X172

【參考文獻】

相關期刊論文 前10條

1 Xiaoyin Xu;Siqing Xia;Lijie Zhou;Zhiqiang Zhang;Bruce E.Rittmann;;Bioreduction of vanadium(V) in groundwater by autohydrogentrophic bacteria:Mechanisms and microorganisms[J];Journal of Environmental Sciences;2015年04期

2 劉風華;宋永會;曾萍;王全紅;宋寶華;韓雙;胡霖;;應用16S rDNA克隆文庫解析好氧顆粒污泥細菌菌群多樣性[J];環(huán)境污染與防治;2013年07期

3 楊華;黃鈞;趙永貴;陳國科;;陶厄氏菌Thauera sp. strain TN9的鑒定及特性[J];應用與環(huán)境生物學報;2013年02期

4 盧文健;楊殿海;郭云;劉巍;;聚磷菌的快速富集及其除磷特性研究[J];環(huán)境污染與防治;2012年06期

5 陳燁;沈耀良;郭海娟;;好氧顆粒污泥的穩(wěn)定性影響因素研究進展[J];水處理技術;2012年02期

6 高景峰;蘇凱;張倩;陳冉妮;王金惠;彭永臻;;不同碳源培養(yǎng)的成熟好氧顆粒污泥的分形表征[J];環(huán)境科學;2010年08期

7 趙霞;趙陽麗;姜峰;馮輝霞;;好氧顆粒污泥的特性及研究進展[J];應用化工;2010年04期

8 劉開朗;王加啟;卜登攀;李旦;于萍;趙圣國;;環(huán)境微生物群落結構與功能多樣性研究方法[J];生態(tài)學報;2010年04期

9 張珍妮;吳曉芙;陳永華;石卉;;DGGE技術在環(huán)境微生物多樣性研究中的應用[J];生物技術通報;2009年12期

10 李建婷;紀樹蘭;劉志培;秦振平;劉纓;楊媛媛;;16S rDNA克隆文庫方法分析好氧顆粒污泥細菌組成[J];環(huán)境科學研究;2009年10期

相關博士學位論文 前2條

1 毛躍建;廢水處理系統(tǒng)中重要功能類群Thauera屬種群結構與功能的研究[D];上海交通大學;2009年

2 閻冰;紅樹林土壤細菌和古菌的16S rDNA多樣性研究[D];華中農(nóng)業(yè)大學;2007年

相關碩士學位論文 前3條

1 戴昕;好氧顆粒污泥工藝運行過程重要功能菌群研究[D];浙江大學;2014年

2 楊輝娜;工業(yè)化厭氧顆粒污泥中的重要菌群及其種群多樣性研究[D];河北科技大學;2012年

3 賈俊濤;T-RFLP分析在細菌調查方面的應用[D];中國海洋大學;2003年

，

本文編號：1343584