植物組培的高效智能LED光照系統(tǒng)設計
本文選題:智能光照系統(tǒng) + 光合有效量子通量密度; 參考:《中國計量學院》2014年碩士論文
【摘要】:光是影響植物組織培養(yǎng)的重要因素。針對傳統(tǒng)熒光燈補光方式存在的能耗高、只有簡單的開關功能等問題,本課題研制了一種高效智能LED光照系統(tǒng),對于降低組培成本,解決組培室光照控制困難等問題具有實際應用意義,具體研究內容如下: (1)系統(tǒng)硬件的實現:包括通信模塊、終端控制器、光照度檢測模塊、LED補光模塊。根據設計要求,構建ZigBee無線網絡,解決組培室連線眾多,管理繁雜的問題;設計基于STM8S單片機和REX3U無線模塊的控制系統(tǒng),實現光強、光質可調;選用硅光電池為光照度檢測模塊的光電探測器,用于監(jiān)測光環(huán)境變化;LED補光模塊由LED光源和恒流驅動電路組成。 (2)系統(tǒng)軟件的開發(fā):包括通信協議、上位機人機界面、下位機執(zhí)行程序。根據ZigBee無線模塊底層要求制定通信傳輸協議,使用C#語言編寫上位機操作界面整體架構和功能模塊,方便用戶對組培室管理,使用C語言編寫下位機各模塊程序,完成信號接收、解析和處理。 (3)基于光度學原理建立光照度與光合有效量子通量密度關系,并用實驗驗證了其正確性。結果表明,紅藍光的光照度與光合有效量子通量密度比值k,其實驗值與理論值相對誤差分別為3.86%、2.66%,光合有效量子通量密度控制誤差在5%之內。系統(tǒng)可提供最大光照強度為90μmol m-2s1,在光強65μmol m-2s1下,,最大紅藍光強比12:1。 (4)應用研制的光照系統(tǒng),在不同光質下對鐵皮石斛組培苗培育試驗。結果表明,光質RB5:1時對鐵皮石斛不定芽數、苗高、節(jié)數、節(jié)間長度促進作用最明顯,不同光質對干物質和葉綠素積累影響差別不大。
[Abstract]:Light is an important factor affecting plant tissue culture. Aiming at the problems of high energy consumption and simple switching function in the traditional fluorescent lamp compensation mode, an efficient and intelligent LED lighting system is developed in this paper, which can reduce the cost of tissue culture. It is of practical significance to solve the difficulty of illumination control in tissue culture room. The specific research contents are as follows: (1) the hardware realization of the system includes communication module, terminal controller, light intensity detection module and LED light compensation module. According to the design requirements, the ZigBee wireless network is constructed to solve the problems of numerous connection and complicated management in the tissue culture room, and the control system based on STM8S single chip microcomputer and REX3U wireless module is designed to realize the light intensity and the light quality can be adjusted. The silicon photocell is selected as the photodetector of illumination detection module, which is used to monitor the change of light environment. The module is composed of LED light source and constant current drive circuit. (2) the development of system software, including communication protocol, PC interface, lower computer execution procedures. According to the requirement of ZigBee wireless module, the communication transmission protocol is established, and the whole structure and function module of the upper computer operating interface are written with C # language, which is convenient for users to manage the tissue culture room, and C language is used to write the programs of each module of the lower computer. Complete signal reception, analysis and processing. (3) based on the photometric principle, the relationship between light intensity and photosynthetic effective quantum flux density is established, and its correctness is verified by experiments. The results show that the relative error between the experimental value and the theoretical value is 3.86 ~ 2.66, and the control error of photosynthetic effective quantum flux density is less than 5%. The system can provide maximum illumination intensity of 90 渭 mol m-2s 1. Under the condition of 65 渭 mol m-2s1 light intensity, the maximum ratio of red and blue light intensity is 12: 1. (4) using the developed light system, the culture experiment of Dendrobium candidum tissue culture seedling was carried out under different light quality. The results showed that the effects of light quality RB5: 1 on adventitious bud number, seedling height, node number and Internode length of Dendrobium candidum were the most obvious, but the effects of different light quality on dry matter and chlorophyll accumulation were not significant.
【學位授予單位】:中國計量學院
【學位級別】:碩士
【學位授予年份】:2014
【分類號】:TM923.34;Q943.1
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