植物組培的高效智能LED光照系統(tǒng)設(shè)計(jì)
本文選題:智能光照系統(tǒng) + 光合有效量子通量密度 ; 參考:《中國(guó)計(jì)量學(xué)院》2014年碩士論文
【摘要】:光是影響植物組織培養(yǎng)的重要因素。針對(duì)傳統(tǒng)熒光燈補(bǔ)光方式存在的能耗高、只有簡(jiǎn)單的開(kāi)關(guān)功能等問(wèn)題,本課題研制了一種高效智能LED光照系統(tǒng),對(duì)于降低組培成本,解決組培室光照控制困難等問(wèn)題具有實(shí)際應(yīng)用意義,具體研究?jī)?nèi)容如下: (1)系統(tǒng)硬件的實(shí)現(xiàn):包括通信模塊、終端控制器、光照度檢測(cè)模塊、LED補(bǔ)光模塊。根據(jù)設(shè)計(jì)要求,構(gòu)建ZigBee無(wú)線(xiàn)網(wǎng)絡(luò),解決組培室連線(xiàn)眾多,管理繁雜的問(wèn)題;設(shè)計(jì)基于STM8S單片機(jī)和REX3U無(wú)線(xiàn)模塊的控制系統(tǒng),實(shí)現(xiàn)光強(qiáng)、光質(zhì)可調(diào);選用硅光電池為光照度檢測(cè)模塊的光電探測(cè)器,用于監(jiān)測(cè)光環(huán)境變化;LED補(bǔ)光模塊由LED光源和恒流驅(qū)動(dòng)電路組成。 (2)系統(tǒng)軟件的開(kāi)發(fā):包括通信協(xié)議、上位機(jī)人機(jī)界面、下位機(jī)執(zhí)行程序。根據(jù)ZigBee無(wú)線(xiàn)模塊底層要求制定通信傳輸協(xié)議,使用C#語(yǔ)言編寫(xiě)上位機(jī)操作界面整體架構(gòu)和功能模塊,方便用戶(hù)對(duì)組培室管理,使用C語(yǔ)言編寫(xiě)下位機(jī)各模塊程序,完成信號(hào)接收、解析和處理。 (3)基于光度學(xué)原理建立光照度與光合有效量子通量密度關(guān)系,并用實(shí)驗(yàn)驗(yàn)證了其正確性。結(jié)果表明,紅藍(lán)光的光照度與光合有效量子通量密度比值k,其實(shí)驗(yàn)值與理論值相對(duì)誤差分別為3.86%、2.66%,光合有效量子通量密度控制誤差在5%之內(nèi)。系統(tǒng)可提供最大光照強(qiáng)度為90μmol m-2s1,在光強(qiáng)65μmol m-2s1下,,最大紅藍(lán)光強(qiáng)比12:1。 (4)應(yīng)用研制的光照系統(tǒng),在不同光質(zhì)下對(duì)鐵皮石斛組培苗培育試驗(yàn)。結(jié)果表明,光質(zhì)RB5:1時(shí)對(duì)鐵皮石斛不定芽數(shù)、苗高、節(jié)數(shù)、節(jié)間長(zhǎng)度促進(jìn)作用最明顯,不同光質(zhì)對(duì)干物質(zhì)和葉綠素積累影響差別不大。
[Abstract]:Light is an important factor affecting plant tissue culture. Aiming at the problems of high energy consumption and simple switching function in the traditional fluorescent lamp compensation mode, an efficient and intelligent LED lighting system is developed in this paper, which can reduce the cost of tissue culture. It is of practical significance to solve the difficulty of illumination control in tissue culture room. The specific research contents are as follows: (1) the hardware realization of the system includes communication module, terminal controller, light intensity detection module and LED light compensation module. According to the design requirements, the ZigBee wireless network is constructed to solve the problems of numerous connection and complicated management in the tissue culture room, and the control system based on STM8S single chip microcomputer and REX3U wireless module is designed to realize the light intensity and the light quality can be adjusted. The silicon photocell is selected as the photodetector of illumination detection module, which is used to monitor the change of light environment. The module is composed of LED light source and constant current drive circuit. (2) the development of system software, including communication protocol, PC interface, lower computer execution procedures. According to the requirement of ZigBee wireless module, the communication transmission protocol is established, and the whole structure and function module of the upper computer operating interface are written with C # language, which is convenient for users to manage the tissue culture room, and C language is used to write the programs of each module of the lower computer. Complete signal reception, analysis and processing. (3) based on the photometric principle, the relationship between light intensity and photosynthetic effective quantum flux density is established, and its correctness is verified by experiments. The results show that the relative error between the experimental value and the theoretical value is 3.86 ~ 2.66, and the control error of photosynthetic effective quantum flux density is less than 5%. The system can provide maximum illumination intensity of 90 渭 mol m-2s 1. Under the condition of 65 渭 mol m-2s1 light intensity, the maximum ratio of red and blue light intensity is 12: 1. (4) using the developed light system, the culture experiment of Dendrobium candidum tissue culture seedling was carried out under different light quality. The results showed that the effects of light quality RB5: 1 on adventitious bud number, seedling height, node number and Internode length of Dendrobium candidum were the most obvious, but the effects of different light quality on dry matter and chlorophyll accumulation were not significant.
【學(xué)位授予單位】:中國(guó)計(jì)量學(xué)院
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2014
【分類(lèi)號(hào)】:TM923.34;Q943.1
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