發(fā)布時(shí)間：2021-10-29 01:09

　　惡性腫瘤分為癌和肉瘤,由于其具有無限增殖、易轉(zhuǎn)移、浸潤性強(qiáng)的特點(diǎn),成為醫(yī)學(xué)上的難關(guān)之一,逐年攀升的發(fā)病率和死亡率嚴(yán)重危害著病人的健康和生命。僅2017年就有新發(fā)癌癥病例168.9萬例,死亡病例60.6萬例,相當(dāng)于每分鐘至少有8人死于癌癥。腫瘤已成為人類健康和生命的重大威脅,因此早期診斷和規(guī)范的治療顯得尤為重要。臨床上常用的治療腫瘤的手段有手術(shù)治療、化學(xué)藥物治療、放射治療治療。手術(shù)作為常見的一種治療方法,對大多數(shù)早期原發(fā)性病灶治療有較好的效果,但對于中、晚期惡性腫瘤的治療效果并不理想,并且手術(shù)治療對人體還會造成較大的創(chuàng)傷,導(dǎo)致患者的免疫力下降,非常容易引起術(shù)后一系列并發(fā)癥。放射治療是指利用不同能量的放射性射線照射腫瘤細(xì)胞,從而達(dá)到殺傷人體病灶部位的腫瘤細(xì)胞的作用。但是,放射性治療的療效受限于腫瘤組織對放射線的敏感程度,并且在放射性射線殺滅和消除腫瘤細(xì)胞的同時(shí),也會對其人體周圍的健康組織細(xì)胞造成一定的損傷。化學(xué)藥物治療是一種全身性的治療方法,但是其選擇性不好,并且長期使用抗腫瘤化學(xué)治療藥物可能會誘發(fā)腫瘤細(xì)胞的耐藥性等問題。由于三大傳統(tǒng)治療手段的臨床效果受到限制,近年來新興的具有非侵入、無創(chuàng)... 

【文章來源】：天津大學(xué)天津市 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：96 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
ABSTRACT
Chapter 1 Introduction
    1.1 Research background and objectives
    1.2 Photothermal therapy (PTT)
        1.2.1 Photothermal agents
    1.3 Photodynamics therapy (PDT)
    1.4 Combination therapy for antitumor
        1.4.1 Combination of PTT and Chemotherapy for antitumor
        1.4.2 Combination of PDT and Chemotherapy for antitumor
        1.4.3 Combination of PTT and PDT for antitumor
    1.5 Multi-modality imaging guided NIR light-induced tumor therapy
    1.6 The design of the project
        1.6.1 The meaning of the project
        1.6.2 Content of the project
Chapter 2 Preparation and characterization of PEG-Mo S_2-Au NPs-Ce6 nanocomposites
    2.1 Introduction
    2.2 Experimental materials and instruments
        2.2.1 Materials
        2.2.2 Instruments
    2.3 Experiment section
        2.3.1 Preparation of PEG-Mo S_2 nanosheets
        2.3.2 Ce6 Loading capacities
        2.3.3 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
        2.3.4 In vitro measurement of photothermal performance
        2.3.5 Drug release
    2.4 Results and discussion
        2.4.1 Synthesis and characterization of PEG-Mo S_2-Au-Ce6 nanocomposites
        2.4.2 Stability and singlet oxygen generation of PEG-Mo S_2-Au-Ce6nanocomposites
        2.4.3 In vitro measurement of photothermal performance
        2.4.4 Drug loading and release
    2.5 Conclusion
Chapter 3 PTT/PDT effect evaluation of PEG-Mo S_2-Au-Ce6 nanocomposites in vitroand in vivo
    3.1 Introduction
    3.2 Experimental materials and instruments
        3.2.1 Materials
        3.2.2 Instruments
    3.3 Experiment section
        3.3.1 Cell cultured experiment
        3.3.2 Cytotoxicity assay
        3.3.3 In vitro cellular uptake study
        3.3.4 Intracellular ROS detection
        3.3.5 Animals and tumors model
        3.3.6 In Vivo NIRF and CT Imaging
        3.3.7 In Vivo photothermal imaging
        3.3.8 In vivo antitumor activity
        3.3.9 Hematoxylin and Eosin (H&E) Staining Analysis
        3.3.10 Statistical analysis
    3.4 Results and Discussion
        3.4.1 MTT assay and Calcein AM/PI co-stained study
        3.4.2 Cellular uptake study and ROS detection
        3.4.3 In vivo NIRF and CT imaging
        3.4.4 In vivo photothermal imaging
        3.4.5 In vivo PTT/PDT for anticancer treatment
    3.5 Conclusion
Chapter 4 Preparation and characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
    4.1 Introduction
    4.2 Materials and instruments
        4.2.1Experimental material
        4.2.2 Instruments
    4.3 Experimental section
        4.3.1 Preparation of Mo S_2 nanodots
        4.3.2 Preparation of PEGlyted Mo S_2@Si O_2 nanoparticles
        4.3.3 Drug loading and CS coating
        4.3.4 Photothermal effect
        4.3.5 Singlet oxygen detection
    4.4 Results and discussion
        4.4.1 Characterization of Alpc-Mo S_2@Si O_2-CS nanocomposites
    4.5 Conclusion
Chapter 5 PTT/PDT effect evaluation of Alpc-Mo S_2@Si O_2-CS nanocomposites in vitroand in vivo
    5.1 Introduction
    5.2 Materials and instruments
        5.2.1 Materials
        5.2.2 Instruments
    5.3 Experiment section
        5.3.1 Detection of intracellular ROS
        5.3.2 Cell culture
        5.3.3 In vitro cytotoxicity evaluation
        5.3.4 In vitro cell experiments
        5.3.5 Animals and tumor models
        5.3.6 NIRF, PA, CT and infrared thermal imaging and ex vivo detection ofROS
        5.3.7 In vivo antitumor efficiency
        5.3.8 Statistics
    5.4 Results and discussion
        5.4.1 In vitro cell experiments
        5.4.2 In vivo NIRF/PA/CT multimodal imaging
        5.4.3 In vivo antitumor activity
    5.5 Conclusion
Chapter 6 Conclusion and future work
    6.1 Conclusion
    6.2 Innovation of this dissertation
    6.3 Future work
References
Published papers and scientific description
Acknowledgments



本文編號：3463671