本文選題：球形金納米粒子　切入點：金納米棒　出處：《東北師范大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：利用DNA分子對金納米粒子進(jìn)行表面修飾和通過DNA的配對實現(xiàn)納米粒子的自組裝的研究已經(jīng)相當(dāng)廣泛�？紤]到DNA的配對是通過堿基的配對完成的,因此單獨采用帶有堿基的有機小分子配體修飾金納米粒子,并且利用核酸堿基間的互補配對(特異性識別-氫鍵作用)實現(xiàn)金納米粒子在溶液中進(jìn)行組裝,并通過控制溫和條件實現(xiàn)解組裝,從而賦予金納米粒子以開關(guān)功能。具體內(nèi)容如下:1、本課題設(shè)計合成了帶有不同長度烷基鏈和硫醇官能團的腺嘌呤(A)和胸腺嘧啶(T)配體。通過檸檬酸鈉還原氯金酸的方法制備了球形金納米粒子,并且利用自主合成的A-C12-SH配體和T-C12-SH配體對球形金納米粒子進(jìn)行表面修飾。實驗表明,配體的濃度影響被修飾的球形金納米粒子的聚集,A-C12-SH配體和T-C12-SH配體的濃度高會引起金納米粒子的聚集。p H值對配體修飾球形金納米粒子的研究表明,在p H6的酸性條件下,A-C12-SH配體修飾金納米球后,會發(fā)生粒子的聚集;在p H4的強酸性條件下,T-C12-SH配體修飾的金納米球會發(fā)生聚集。經(jīng)過A-C12-SH配體和T-C12-SH配體修飾后的金納米球之間可以發(fā)生自組裝,并且通過調(diào)節(jié)溶液的酸堿性,可以控制金納米球的組裝與解組裝。2、通過晶種生長法,控制生長溶液中Ag NO3的含量制備出了縱向吸收峰為780nm的金納米棒,并且利用自主合成的A-C12-SH配體和T-C12-SH配體對金納米棒進(jìn)行表面修飾。通過紫外分析得出在p H≤6的酸性條件下,經(jīng)過A-C12-SH配體修飾的GNR會發(fā)生橫向聚集;在p H≥10的強堿性條件下,T-C12-SH配體修飾的GNR發(fā)生橫向聚集。將帶有T-C12-SH配體的金納米棒和帶有A-C12-SH配體的金納米球進(jìn)行組裝,當(dāng)修飾的金納米球比例大于修飾的金納米棒,組裝會形成團聚,紫外吸收不再有縱向吸收峰;當(dāng)修飾的金納米棒的比例大于修飾的金納米球的比例,組裝后的紫外吸收峰仍然有縱向吸收峰;并且酸性和堿性條件都會加大組裝程度。
[Abstract]:The surface modification of gold nanoparticles using DNA molecules and the self-assembly of gold nanoparticles by DNA pairing have been extensively studied. Considering that the pairing of DNA is accomplished by base pairing, Therefore, gold nanoparticles were modified by organic small molecular ligands with bases alone, and the gold nanoparticles were assembled in solution by complementary pairing between nucleic acid bases (specific recognization-hydrogen bond interaction). And by controlling the mild conditions to realize the disassembly, The specific contents are as follows: 1. In this paper, we designed and synthesized adenine (A) and thymine (Thymine) ligands with different alkyl chains and mercaptan functional groups. Chloruronic acid was reduced by sodium citrate. The spherical gold nanoparticles were prepared by the method of. The spherical gold nanoparticles were modified by self-synthesized A-C12-SH ligands and T-C12-SH ligands. The effect of concentration of ligand on the aggregation of gold nanoparticles, such as the concentration of A-C12-SH ligands and T-C12-SH ligands, will lead to the aggregation of gold nanoparticles. After modification of gold nanospheres with A-C12-SH ligands under acidic conditions of pH _ 6, the aggregation of gold nanoparticles will occur. The gold nanospheres modified by T-C12-SH ligands can aggregate under the strong acidity of pH4. The gold nanospheres modified by A-C12-SH ligands and T-C12-SH ligands can be self-assembled and can be modified by adjusting the acidity and basicity of the solution. The gold nanorods with a longitudinal absorption peak of 780nm were prepared by controlling the content of Ag NO3 in the growth solution by controlling the assembly and unassembly of gold nanospheres. The gold nanorods were modified by self-synthesized A-C12-SH ligands and T-C12-SH ligands. UV analysis showed that the GNR modified by A-C12-SH ligands would aggregate laterally under acidic pH 鈮，

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