本文關(guān)鍵詞： 金納米顆粒 牛血清蛋白(BSA) 單分散結(jié)合 結(jié)合位點(diǎn) 榫hY嵌合　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：隨著納米技術(shù)的發(fā)展,·納米顆粒由于其優(yōu)質(zhì)的物理和化學(xué)性質(zhì),使其在科學(xué)研究及工業(yè)領(lǐng)域得到了廣泛的關(guān)注。然而,當(dāng)一些功能化的納米顆粒應(yīng)用于生物體內(nèi)時(shí),生物體內(nèi)的蛋白迅速的吸附在納米顆粒的表面,形成納米顆粒-蛋白質(zhì)冠的結(jié)構(gòu),導(dǎo)致了納米顆粒預(yù)先設(shè)計(jì)功能的改變。因此,探索蛋白質(zhì)與納米顆粒的作用機(jī)制對(duì)功能性納米顆粒在生物體內(nèi)功能的發(fā)揮是至關(guān)重要的。本文以牛血清蛋白為例,通過(guò)牛血清蛋白(BSA)與納米顆粒的物理吸附、蛋白酶酶解、結(jié)合聚丙烯酰胺凝膠電泳(PAGE)和基質(zhì)輔助激光解吸／電離質(zhì)譜-飛行時(shí)間質(zhì)譜(MALDI-TOF-MS)的鑒定,我們發(fā)現(xiàn),BSA中有107個(gè)氨基酸、分子量大小約為12.8kDa的肽鏈為BSA與AuNP的結(jié)合位點(diǎn)。為了進(jìn)一步解釋為什么會(huì)有如此長(zhǎng)的肽段被金納米顆粒的表面所保護(hù),以至于不受蛋白酶酶解,我們提出了一種新穎的機(jī)械榫眼和榫舌嵌合的結(jié)合模式,其中充當(dāng)榫舌的結(jié)合肽插入到疏水槽金納米粒子的表面上的榫眼中。我們選取分子量小于BSA的牛血紅蛋白(Hgb),通過(guò)化學(xué)連接的方式并結(jié)合分子篩以及動(dòng)態(tài)光散色儀兩種方式來(lái)認(rèn)證該模型的可能性,另外小分子布洛芬、SDS與BSA競(jìng)爭(zhēng)結(jié)合納米金顆粒的實(shí)驗(yàn)來(lái)進(jìn)步證明該模型的正確性。
[Abstract]:With the development of nanotechnology, nanoparticles have attracted wide attention in the field of scientific research and industry because of their excellent physical and chemical properties. However, when some functional nanoparticles are used in organisms, Proteins are rapidly adsorbed on the surface of nanoparticles, forming the structure of nanocrystalline protein crowns, resulting in changes in the pre-designed function of nanoparticles. It is very important to explore the mechanism of action between protein and nanoparticles for the function of functional nanoparticles in vivo. In this paper, bovine serum protein (BSA) is used as an example to study the physical adsorption and enzymatic hydrolysis of bovine serum protein (BSA) with nanoparticles. Combined with the identification of polyacrylamide gel electrophoresis (page) and matrix assisted laser desorption / ionization mass spectrometry (MALDI-TOF-MS), we found that there are 107 amino acids in BSA. A peptide chain with a molecular weight of about 12.8kDa is the binding site between BSA and AuNP. To further explain why such a long peptide segment is protected from proteolysis by the surface of gold nanoparticles, We propose a novel mechanical mortise and tongue-tongue-inlay mode. The binding peptide, which acts as a tenon, is inserted into the tenon hole on the surface of gold nanoparticles. We select bovine hemoglobin (HGB) with molecular weight less than BSA, and combine it with molecular sieve and dynamic photochromograph by chemical bonding. There are two ways to authenticate the possibility of the model, In addition, small molecule ibuprofen SDS competes with BSA in the experiment of gold nanoparticles to prove the correctness of the model.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;O614.123

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin[J];Chinese Science Bulletin;2012年10期

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本文編號(hào)：1552710