本文關鍵詞：基于氧化石墨烯的納米雜化材料在腫瘤多模成像和治療中的應用　出處：《上海師范大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 氧化石墨烯 鎢酸錳 納米雜化材料 多模式成像 光熱/藥物協(xié)同治療

【摘要】：石墨烯(Graphene)是一種由碳原子構成的新型材料,是由碳原子以sp2雜化軌道組成六角型呈蜂巢晶格平面結構、只有一個碳原子厚度的二維材料。氧化石墨烯(Graphene Oxide)是石墨烯的一種重要的衍生物,也被稱為功能化的石墨烯。它的結構與石墨烯大體相同,只是在二維基面上連有一些官能團。氧化石墨烯具有很大的比表面積和豐富的表面缺陷,無機納米顆粒可以通過原位生長或后修飾負載到氧化石墨烯表面。通過良好的表面功能化修飾可提高GO在生物體內的穩(wěn)定性以及可控性,從而可以獲得良好生物相容性的基于氧化石墨烯的納米雜化材料。氧化石墨烯納米雜化材料不僅可用于腫瘤的光熱治療和作為載體進行藥物和基因輸送,還可被用作造影劑應用在生物多模式成像中,包括CT成像、磁共振成像(MRI)以及光聲成像(PAI)等。目前,己經(jīng)有大量文章報道了關于GO及其衍生物在生物醫(yī)學方面的廣泛應用。本論文主要研究在氧化石墨烯表面負載Mn WO_4或Bi_2Se_3納米粒子,并通過一鍋法在納米材料表面修飾改善生物相容性的聚乙二醇(PEG)或聚乙烯吡咯烷酮(PVP)進行改性。通過π-π相互作用和靜電相互作用得到負載鹽酸阿霉素(DOX)的氧化石墨烯基納米雜化材料,可以被應用在光熱治療或光熱/藥物協(xié)同治療。第一章綜述了氧化石墨烯的結構、性質、制備方法、表面功能化修飾以及其在生物成像和腫瘤治療上的應用。第二章研究了GO/Mn WO_4/PEG納米雜化材料的制備、表征以及其在細胞、活體中的生物成像和治療方面的應用。通過高溫加熱一步合成的GO/MnWO_4/PEG納米雜化材料,具有良好的形貌、較小的尺寸和良好的分散性。溶液水平表征結果表明,材料在近紅外有較強吸收,在激光照射下有良好的升溫效果。GO/Mn WO_4/PEG納米雜化材料表面負載抗癌藥物DOX后,在酸性環(huán)境中藥物釋放較多,并且在光熱條件下,其藥物釋放的能力會得到一定增強。同時,GO/MnWO_4/PEG納米雜化材料在溶液水平表現(xiàn)出良好的MRI/CT/PA造影性能,并且在相應的活體成像上也取得了良好的造影效果。GO/MnWO_4/PEG納米雜化材料在體外實驗中表現(xiàn)出了良好的生物相容性,并且對4T1細胞有較好的光熱/藥物殺死效果�；诓牧狭己玫纳锵嗳菪院腕w外治療效果,將GO/MnWO_4/PEG納米雜化材料應用在活體荷瘤小鼠的腫瘤治療上,在治療期間,腫瘤基本被抑制,取得了良好的光熱/藥物協(xié)同治療效果。因此,GO/MnWO_4/PEG納米雜化材料可以應用于生物體內的多模成像和光熱/藥物協(xié)同治療。第三章研究了GO/Bi_2Se_3/PVP納米雜化材料在生物體內的CT/PA成像和腫瘤的光熱治療。通過尾靜脈向荷瘤小鼠體內注射該材料,然后對小鼠進行CT成像掃描,可在小鼠腫瘤部位觀察到一定的成像效果。GO/Bi_2Se_3/PVP納米材料在溶液水平的光聲成像效果良好,可以觀察到隨著材料溶液濃度增大,材料成像對比度增強,后將其應用于活體荷瘤小鼠的光聲成像并取得了良好的成像效果,說明該材料可作為CT/PA雙模式成像造影劑。同時,研究了GO/Bi_2Se_3/PVP納米雜化材料對荷瘤小鼠腫瘤的光熱消融效果,得到了較好的光熱治療效果。因此,GO/Bi_2Se_3/PVP納米雜化材料可以作為生物體內的CT/PA成像造影劑和癌癥光熱治療劑。第四章總結了本論文中的各項數(shù)據(jù)和結果,并對基于GO的納米雜化材料在生物醫(yī)藥上的應用進行展望。
[Abstract]:Shi Moxi (Graphene) is a kind of new material composed of carbon atoms, is made of carbon atoms to SP2 hybrid orbitals consisting of six angle type honeycomb lattice plane structure, the two-dimensional material is only one carbon atom. The thickness of graphene oxide (Graphene Oxide) is a kind of important graphene derivatives, also called graphite as a function of the graphene and graphene structure. It is roughly the same, only in the two-dimensional surface with some functional groups. The graphene oxide has large surface area and abundant surface defects, inorganic nano particles can be obtained by in situ growth or after modification of the load to the surface of graphene oxide. By surface modification function can improve the stability of GO in organisms and controllability, which can obtain good biocompatibility of nano hybrid materials based on graphene. The graphene oxide nano hybrid materials can be used not only to swell Photothermal therapy of tumor and as a carrier of drug and gene delivery, can also be used as contrast agent used in biological pattern imaging, including CT imaging, magnetic resonance imaging (MRI) and photoacoustic imaging (PAI). At present, there are a large number of articles have been reported on the application of GO and its derivatives in biomedicine the. This paper mainly studies the load Mn WO_4 or Bi_2Se_3 nanoparticles on the surface of graphene oxide and modified by one pot method, improve the biocompatibility of the nano materials in the surface of polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP) was modified by tt-tt and electrostatic interactions are supported (doxorubicin hydrochloride DOX) graphene oxide based nano hybrid materials can be used in photothermal therapy or thermal / drug therapy. The first chapter summarizes the collaborative structure, graphene oxide, preparation method, surface modification And its application in biological imaging and tumor therapy. The second chapter studies the preparation of GO/Mn WO_4/PEG nano hybrid materials, characterization and its application in living cells, biological imaging and therapy. By heating one step synthesis of GO/MnWO_4/PEG nano hybrid materials, has good appearance, small size and good the dispersion. The characterization results show that the level of solution, material has strong absorption in the near infrared, under the laser irradiation load anticancer drug DOX good heating effect of.GO/Mn WO_4/PEG nano hybrid material surface, in the acidic environment of drug release more, and in the thermal conditions, the drug release will be enhanced. At the same time, GO/MnWO_4/PEG nano hybrid materials in solution MRI/CT/PA contrast levels showed a good performance, and the corresponding in vivo imaging has achieved favorable angiography effect. GO/MnWO_4/PEG nano hybrid materials in vitro showed good biocompatibility, and good thermal / drugs killing effect on 4T1 cells in vitro. The compatibility and treatment effect of good biological material based on tumor GO/MnWO_4/PEG nano hybrid materials used in the in vivo tumor bearing mice in the treatment, during the treatment of cancer is suppressed, and achieved good thermal / drug synergistic therapeutic effect. Therefore, the multimode imaging and photothermal / GO/MnWO_4/PEG nano hybrid materials can be used in the in vivo drug synergistic treatment. The third chapter studies the thermal treatment of GO/Bi_2Se_3/PVP nano hybrid materials in vivo CT/PA imaging and tumor. Through tail vein injection to mice the material, then the CT imaging of mice can be observed in mouse tumor imaging effect of.GO/Bi_2Se_3/PVP nano materials to a certain The material of photoacoustic imaging effect in the solution level is good, can be observed with the material concentration increased, the material image contrast enhancement, after its application in tumor bearing mice in vivo photoacoustic imaging and achieved good imaging effect, indicating that the material can be used as a CT/PA dual mode imaging contrast agent. At the same time, study thermal GO/Bi_2Se_3/PVP nano hybrid materials of tumor ablation effect, obtained better effect of photothermal therapy. Therefore, GO/Bi_2Se_3/PVP nano hybrid materials can be used as the organism CT/PA imaging contrast agent and cancer photothermal therapeutic agent. The fourth chapter summarizes the data and results presented in this paper, and the prospect of application in biology medicine on the nano hybrid materials based on GO.

【學位授予單位】：上海師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R730;TB383.1

【參考文獻】

相關期刊論文 前1條

1 Pilger FRANK;;Poly(ethylene glycol) conjugated nano-graphene oxide for photodynamic therapy[J];Science China(Chemistry);2010年11期

，

本文編號：1373411