發(fā)布時間：2018-04-28 09:10

  本文選題：犬 + 犬瘟熱　； 參考：《湖南農(nóng)業(yè)大學(xué)》2016年碩士論文

【摘要】：近年來,隨著長沙市養(yǎng)犬量的增多,犬瘟熱的發(fā)病也不斷在增加,導(dǎo)致此病越來越難預(yù)防；犬瘟熱感染早期臨床癥狀不明顯以及臨床經(jīng)過期癥狀復(fù)雜,是導(dǎo)致臨床診斷難的主要原因。本文通過兩個相關(guān)實驗對此進行研究。一是長沙市犬瘟熱感染情況調(diào)查。犬瘟熱流行特點與季節(jié)、犬齡、免疫情況等都存在密切關(guān)系。為了進一步研究犬瘟熱發(fā)病與各因素的關(guān)系,進一步做好預(yù)防犬瘟的工作,把長沙市內(nèi)五個區(qū)的犬瘟感染情況進行了統(tǒng)計分析。結(jié)果表明：犬瘟發(fā)病率與地區(qū)間差異有關(guān),經(jīng)濟條件相對較落后的地區(qū)發(fā)病率高；與免疫情況有關(guān),完成3次免疫以上的犬發(fā)病率明顯低于未免疫和免疫不完全的犬；與季節(jié)變化有關(guān),一年中的3-4月和10-11月發(fā)病率明顯高于其他月份；與犬齡有關(guān),2-10月齡犬的發(fā)病率最高；與犬的來源有關(guān),來源不明的犬發(fā)病率高于來源清楚犬；與疫苗品牌有關(guān),進口疫苗發(fā)病率低于國產(chǎn)苗。根據(jù)調(diào)查結(jié)果,作者認(rèn)為犬瘟熱的流行是多種因素造成的,因此在預(yù)防犬瘟熱方面,應(yīng)完善犬瘟熱的免疫制度,選擇好的疫苗,定期免疫接種；犬類養(yǎng)殖應(yīng)該規(guī)范化、科學(xué)化,定期消毒,及時控制病毒擴散,及時隔離,防止全面爆發(fā)。二是犬瘟熱的RT-PCR診斷。臨床普遍采用膠體金技術(shù)檢測犬瘟熱感染,而對檢測結(jié)果的準(zhǔn)確性一直存在質(zhì)疑,為了進一步調(diào)查其檢測的準(zhǔn)確性；進一步研究誤診犬瘟熱的原因,作者采取RT-PCR檢測方法來驗證膠體金檢測的準(zhǔn)確率,并鑒別診斷誤診案例。結(jié)果表明：隨機抽取的41份膠體金檢測結(jié)果為陰性的病料中,RT-PCR檢測出3份陽性,膠體金誤診率為7.32%(3/41)；誤診的原因是膠體金檢測出假陰性、感染犬早期癥狀不明顯、繼發(fā)感染犬瘟、臨床癥狀多樣。作者認(rèn)為,犬瘟感染早期,在癥狀不明顯時應(yīng)該采用RT-PCR方法診斷,而在臨床經(jīng)過期采用膠體金診斷為宜。
[Abstract]:In recent years, with the increase of canine distemper in Changsha, the incidence of canine distemper is increasing, which makes the disease more and more difficult to prevent, the early clinical symptoms of canine distemper infection are not obvious and the clinical symptoms are complicated. It is the main cause of the difficulty in clinical diagnosis. In this paper, two related experiments are carried out to study this. The first is the investigation of canine distemper infection in Changsha. The epidemic characteristics of canine distemper are closely related to season, dog age and immune status. In order to further study the relationship between canine distemper and other factors and to prevent canine blast, the infection of canine distemper in five districts of Changsha city was analyzed statistically. The results showed that the incidence of canine plague was related to regional differences, and the incidence of canine plague was higher in areas with relatively backward economic conditions, and was significantly lower in dogs with more than three rounds of immunization than that in dogs without or without complete immunization. The incidence in March-April and October-November of a year was significantly higher than that in other months, the highest incidence was in dogs of 2-10 months of age, and the incidence was higher in dogs of unknown origin than that in dogs of unknown origin. The incidence of imported vaccines is lower than that of domestic vaccines. According to the results of the investigation, the author thinks that the prevalence of canine distemper is caused by many factors. Therefore, in the prevention of canine distemper, we should perfect the immune system of canine distemper, select a good vaccine and vaccinate regularly, and canine breeding should be standardized and scientific. Regular disinfection, timely control of the spread of the virus, timely isolation, to prevent an overall outbreak. The second is the RT-PCR diagnosis of canine distemper. Colloidal gold technique is widely used to detect canine distemper infection in clinic. However, the accuracy of the detection results has always been questioned. In order to further investigate the accuracy of the detection and further study the causes of misdiagnosis of canine distemper, The RT-PCR method was used to verify the accuracy of colloidal gold detection and to identify the misdiagnosis cases. The results showed that 3 of 41 samples with negative colloidal gold were detected by RT-PCR, and the misdiagnosis rate of colloidal gold was 7.32 / 41. The reason of misdiagnosis was that colloidal gold was false negative, the early symptoms of infected dog were not obvious, and the secondary infection was canine plague. Clinical symptoms are varied. In the early stage of canine plague infection, RT-PCR method should be used when the symptoms are not obvious, and colloidal gold should be used in clinical diagnosis.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S858.292

【參考文獻】

相關(guān)期刊論文 前10條

1 徐瑞濤;;保定地區(qū)犬瘟熱病毒感染及診治調(diào)查[J];黑龍江畜牧獸醫(yī);2013年02期

2 劉大飛;姜一f，

本文編號：1814647