【摘要】：目的:探討5周間歇性亞極限強(qiáng)度運(yùn)動引起大鼠睪酮水平下降的可能機(jī)制。方法:8周齡SD大鼠分為運(yùn)動干預(yù)組和安靜對照組,運(yùn)動組大鼠以尾部負(fù)重的方式(6%體重)進(jìn)行4min×5次游泳訓(xùn)練,間隔90 s,上、下午各進(jìn)行1次訓(xùn)練,每周6天,分別持續(xù)1、2、3、4、5周。各組大鼠末次訓(xùn)練結(jié)束48 h后取材,測定StAR、PKA、pCREB、SF-1等相關(guān)蛋白及基因mRNA變化情況。結(jié)果:1)運(yùn)動干預(yù)組血睪酮水平后2周下降幅度較大,第5周血睪酮水平與安靜對照組相比下降了70%(P0.01)。2)運(yùn)動干預(yù)組大鼠StAR mRNA在第5周出現(xiàn)了顯著性下降(P0.05)。3)大鼠睪丸組織PKA、pCREB、SF-1蛋白表達(dá)以及SF-1 mRNA表達(dá)在后2周出現(xiàn)了顯著下降(P0.05)。結(jié)論:5周間歇性亞極限強(qiáng)度運(yùn)動引起大鼠睪丸間質(zhì)細(xì)胞內(nèi)St AR及其相關(guān)調(diào)控因子PKA、pCREB、SF-1等表達(dá)下降,進(jìn)而引起膽固醇轉(zhuǎn)運(yùn)受限,這可能是導(dǎo)致睪酮合成和血液睪酮水平下降的原因之一。
[Abstract]:Aim: to investigate the possible mechanism of the decrease of testosterone level in rats induced by intermittent submaximum intensity exercise for 5 weeks. Methods: 8-week-old SD rats were divided into two groups: exercise intervention group and quiet control group. Rats in exercise group received 4min 脳 5 swimming exercises in the form of tail weight loading (6% body weight) for 90 s, and once in the afternoon, 6 days a week. The two groups lasted for 5 weeks. The changes of StAR,PKA,pCREB,SF-1 and gene mRNA were measured 48 h after the last training. Results: 1) the serum testosterone level in the exercise intervention group decreased significantly 2 weeks after treatment. The serum testosterone level decreased by 70% (P0.01) in the 5th week compared with that in the quiet control group (P0.01). The StAR mRNA of the rats in the exercise intervention group decreased significantly at the 5th week (P0.05). 3) the PKA,pCREB, of testis in the exercise intervention group was significantly decreased (P0.05). The expression of SF-1 protein and SF-1 mRNA decreased significantly after 2 weeks (P0.05). Conclusion: during 5 weeks of intermittent submaximum intensity exercise, the expression of St AR and its related regulatory factors PKA,pCREB,SF-1 in rat testis stromal cells decreased, and then cholesterol transport was restricted. This may be one of the reasons for testosterone synthesis and a decrease in blood testosterone levels.
【作者單位】： 國家體育總局體育科學(xué)研究所;常州市體育醫(yī)療科研所;
【基金】：國家體育總局體育科學(xué)研究所基本科研業(yè)務(wù)經(jīng)費(fèi)資助項目(基本16-28)
【分類號】：G804.2

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