本文選題：肥胖大鼠 + 低氧訓(xùn)練�。� 參考：《安徽師范大學(xué)》2017年碩士論文

【摘要】：研究目的研究低氧環(huán)境下間歇耐力運(yùn)動(dòng)對(duì)肥胖大鼠體成分、瘦素、胰島素敏感性的影響,探討低氧環(huán)境下間歇耐力運(yùn)動(dòng)減體重的可能原因,為后期研究低氧減體重提供理論依據(jù)和方法指導(dǎo)。研究方法6周齡雄性SPF級(jí)SD大鼠203只,隨機(jī)挑選25只用普通飼料喂養(yǎng),178只用高脂飼料喂養(yǎng),18周后,按高脂飼養(yǎng)大鼠體重增量大于普通飼養(yǎng)大鼠體重增量均值加上1倍標(biāo)準(zhǔn)差作為肥胖標(biāo)準(zhǔn)篩選出肥胖大鼠。隨后從普通飼料喂養(yǎng)大鼠中挑選8只作為對(duì)照組,即常氧安靜組(NS組),從經(jīng)過(guò)適應(yīng)性訓(xùn)練的肥胖大鼠中篩選出56只平均分為7組,保證每組大鼠體重均值基本一致,隨機(jī)分為肥胖低住安靜組(LNS組)、肥胖高住安靜組(HNS組)、肥胖低住低練持續(xù)組(LLC組)、肥胖低住低練間歇組(LLI組)、肥胖低住高練間歇組(LHI組)、肥胖高住低練間歇組(HLI組)、肥胖高住高練間歇組(HHI)。訓(xùn)練組大鼠中持續(xù)訓(xùn)練組進(jìn)行4周動(dòng)物跑臺(tái)持續(xù)耐力訓(xùn)練,間歇訓(xùn)練組進(jìn)行4周間歇耐力訓(xùn)練。持續(xù)組一次性完成訓(xùn)練,間歇組分為兩次完成,兩次訓(xùn)練間隔6小時(shí)。常氧環(huán)境下訓(xùn)練每周5天每天60分鐘,強(qiáng)度為22米/分、低氧環(huán)境下訓(xùn)練每周5天每天60分鐘,強(qiáng)度為20米/分。試驗(yàn)期間每周一上午在大鼠禁食12小時(shí)、禁水2小時(shí)稱量大鼠體重,每周日晚8點(diǎn)收集大鼠飼料,計(jì)算大鼠的食物攝入量。運(yùn)動(dòng)干預(yù)4周后,大鼠休息12小時(shí)后處死大鼠,腹主動(dòng)脈取血待測(cè),并取大鼠腎周、附睪脂肪、腓腸肌、心肌在生理鹽水中洗凈、濾紙吸干水分電子天平稱量重量。研究結(jié)果1.實(shí)驗(yàn)四周末HHI組、LHI組、HLI組大鼠體重均顯著性低于LNS組與HNS組(P0.05),HHI組體重顯著低于LLC組和LLI組(P0.05)。HHI組每一周的體重降幅顯著高于HLI組和LHI組(P0.05),HHI組的總體重降幅高于LLC組和LLI組但差異不顯著(P0.05)。2.四周實(shí)驗(yàn)后HHI組、HLI組、LHI組大鼠腎周脂肪和附睪脂肪均低于HNS組與LNS組,HHI組腎周脂肪、附睪脂肪相對(duì)值顯著低于LLC組(P0.05);HHI組、HLI組、LHI組大鼠脂體比低于HNS組與LNS組但無(wú)顯著差異(P0.05),所有低氧間歇運(yùn)動(dòng)組大鼠體脂比均低于常氧運(yùn)動(dòng)組。HHI組的體脂比顯著低于HLI組、LHI組(P0.05)。3.四周實(shí)驗(yàn)后,HHI組、HLI組LHI組血清總膽固醇水平低于LNS組、各低氧運(yùn)動(dòng)組的總膽固醇水平亦低于常氧運(yùn)動(dòng)組差異不顯著(P0.05);血清甘油三酯(TG)含量HHI組、HLI組、LHI組均于LNS組,HHI組、LHI組甘油三酯水平略低于LLC組、LLI組但差異不顯著(P0.05);實(shí)驗(yàn)四周后,HHI組、LHI組、HLI組低密度脂蛋白水平低于LNS組,HHI組低密度脂蛋白水平低于LLC組LLI組;四周實(shí)驗(yàn)后HHI組、LHI組HLI組高密度脂蛋白水平高于LNS組,LHI組HLI組高密度脂蛋白水平高于LLC組。4.實(shí)驗(yàn)四周后LHI組和HHI組的瘦素水平低于LNS組和HNS組,HHI組、HLI組、LHI組的瘦素水平低于LNS組與HNS組但無(wú)顯著性差異(P0.05)。低氧運(yùn)動(dòng)組的胰島素水平低于常氧運(yùn)動(dòng)組。4周實(shí)驗(yàn)后HHI組HLI組血糖水平低于LNS組,HHI組、HLI組、LHI組的血糖水平低于LLC組LLI組;實(shí)驗(yàn)4周后,HHI組、LHI組的胰島素指數(shù)低于LNS組HNS組,但與HNS組無(wú)顯著性差異,HHI組LHI組和LHI組的胰島素抵抗指數(shù)低于LNS組,低氧運(yùn)動(dòng)組胰島素抵抗指數(shù)低于常氧運(yùn)動(dòng)組,但無(wú)顯著性差異(P0.05)。結(jié)論從降低體重的幅度來(lái)看,HHI組HLI組LHI組LLI組;低氧環(huán)境下間歇耐力運(yùn)動(dòng)對(duì)肥胖機(jī)體體成分有良好影響,均能有效減少肥胖大鼠脂肪含量,其中高住高練間歇訓(xùn)練方式對(duì)體成分影響最佳;低氧環(huán)境下間歇耐力運(yùn)動(dòng)均能降低肥胖大鼠血清TG、LDL-C水平,提高HDL-C水平,對(duì)血脂產(chǎn)生良好影響;低氧環(huán)境下間歇耐力運(yùn)動(dòng)能夠有效的降低肥胖機(jī)體的瘦素水平,對(duì)肥胖機(jī)體的瘦素水平有較好的影響;低氧環(huán)境下間歇耐力運(yùn)動(dòng)能夠降低肥胖機(jī)體的胰島素水平及胰島素指數(shù),影響肥胖機(jī)體的胰島素敏感性。
[Abstract]:Objective to study the effect of intermittent endurance exercise on body composition, leptin and insulin sensitivity in hypoxic rats and to explore the possible causes of weight loss in intermittent endurance exercise under hypoxic environment, and provide theoretical basis and method guidance for the later study of hypoxic weight reduction. 203 male SPF rats of 6 weeks' age were selected to select 25 randomly. After only feeding with ordinary feed, 178 only were fed with high fat diet. After 18 weeks, the weight increment of rats fed by high fat feeding rats was larger than that of normal feeding rats plus 1 times the standard deviation of 1 times as a standard of obesity. Then 8 of the rats were selected as control group, that is, the normal oxygen quiet group (group NS). The average weight of 56 obese rats was divided into 7 groups, which ensured that the average weight of the rats in each group was basically the same. It was randomly divided into the obese and low living quiet group (group LNS), the obese and quiet group (group HNS), the fat low living and low practice group (group LLC), the fat low living and low training intermittent group (group LLI), the fat low high practice interval group (group LHI), the obesity and low practice. In the intermittent group (group HLI), the obese and high training interval group (HHI). The continuous training group was trained for 4 weeks in the training group and the intermittent training group had 4 weeks of intermittent endurance training. The continuous group was completed once in one time, the intermittent group was divided into two times and the two training interval was 6 hours. The training group was trained for 60 minutes a day for 5 days a week. The intensity was 22 m / min and 5 days a week, 60 minutes a week and 20 m / min in the low oxygen environment. The rats were fasted for 12 hours in rats every Monday morning, and the rats were weighed for 2 hours without water. The rat feed was collected at 8 o'clock every Sunday night, and the food intake of rats was calculated. After 4 weeks of exercise intervention, rats were killed and abdominal initiative after rest 12 hours after the rest of the rats. Abdominal initiative was taken. The body weight of the HHI group, LHI group and HLI group was significantly lower than that of group LNS and HNS group (P0.05), and the weight of group HHI was significantly lower than that of group LLC and LLI group (P0.05).HHI group every week in group HHI. The weight reduction of body weight was significantly higher than that of group HLI and group LHI (P0.05). The total weight loss of group HHI was higher than that of group LLC and LLI group, but the difference was not significant (P0.05) in group HHI, HLI group and LHI group was lower than that in HNS group and epididymal fat, and the relative value of epididymal fat was significantly lower than that of group of HNS and epididymis. The fat body ratio in the rats was lower than that of the HNS group and the LNS group (P0.05). The body fat ratio of all hypoxic intermittent exercise groups was lower than that of the.HHI group of the normooxy group was significantly lower than that of the HLI group. After the LHI group (P0.05).3. around the experiment, the serum total cholesterol level of the HHI group and HLI group LHI group was lower than that of the LNS group, and the total cholesterol level in the hypoxia exercise group was also low. There was no significant difference in the aerobics group (P0.05), the content of serum triglyceride (TG) in group HHI, group HLI and LHI in group LNS, HHI group and LHI group were slightly lower than that of group LLC, and LLI group was not significant (P0.05), and the level of low density lipoprotein in HHI group, group, and low density lipoprotein was lower than that in the group of LLI, and the level of low density lipoprotein was lower than that of the group. The level of HDL in group HLI and group LHI was higher than that of group LNS, and the level of high density lipoprotein in group HLI of group LHI was higher than that of group LLC and.4. experiment in group LHI, and the level of leptin in group HLI and HHI group was lower than that of LNS group and HNS group. The level of blood glucose in group HLI of group HHI was lower than that of group LNS after.4 week in group HLI. The blood glucose level of group HHI, HLI and LHI was lower than that of group LLI. After 4 weeks, the insulin index of HHI group and LHI group was lower than that of LNS group, but there was no significant difference between the group and the group, the insulin resistance index was lower than that of the group and the hypoxia group. The insulin resistance index in the exercise group was lower than that of the normoxic exercise group, but there was no significant difference (P0.05). Conclusion from the margin of weight reduction, the group LHI of group HLI was LLI in group HHI, and the intermittent endurance exercise under the low oxygen environment had a good effect on the body composition of the obese body, which could effectively reduce the fat content in the obese rats. The effect of body composition is the best. Intermittent endurance exercise in low oxygen environment can reduce the serum TG, LDL-C level and increase the level of HDL-C in obese rats. The intermittent endurance exercise can effectively reduce the level of leptin in obese body, have a good influence on the leptin level of obese body, and the interval of hypoxia is intermittent. Endurance exercise can reduce the insulin level and insulin index of obese body, and affect the insulin sensitivity of obese body.
【學(xué)位授予單位】：安徽師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：G804.2

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