本文選題：有氧運動 + 炎癥反應��； 參考：《揚州大學》2017年碩士論文

【摘要】：研究目的:探討有氧運動對心肌梗死(MI)后大鼠心肌組織抑炎因子白介素-4(IL-4)、白介素-10(IL-10)分泌情況及信號傳導蛋白和轉(zhuǎn)錄激活物6(STAT6)磷酸化水平的影響,以及STAT6在炎癥反應中的作用機制。研究方法:選用30只雄性8周齡的SD大鼠,隨機分為假手術(shù)組(Sham)、心肌梗死組(MI)、梗死+運動組(ME)。以冠狀動脈左前降支(LAD)結(jié)扎術(shù)構(gòu)建MI模型,運動方式為間歇有氧運動。運動方案:1周的適應性運動后,10m/min熱身10min,然后以20m/min運動8min,再以15 m/min運動2 min,交替4個循環(huán),最后20 m/min運動8min,10 m/min運動2 min后結(jié)束。每天訓練1次,5 d/w,共訓練8 w。以二維超聲心動圖檢測大鼠心臟室壁厚度、射血功能;采用ELISA的方法檢測大鼠心肌組織IL-4、IL-10的分泌水平;Western blot檢測心肌組織p-STAT6、t-STAT6的表達水平。研究結(jié)果:(1)MI手術(shù)使大鼠心臟心室壁變薄,射血功能降低,射血分數(shù)(EF)與Sham組相比明顯減小(P≤0.01);8周有氧運動使MI大鼠的室壁厚度增加、射血功能加強,ME組舒張末期左室后壁厚度(LVPWd)與舒張末期室間隔厚度之和明顯高于MI組(P≤0.05),ME組EF比MI組明顯升高(P≤0.05)。(2)MI組心重/體重比值(HW/BW)顯著低于Sham組(P≤0.05),ME組與MI組相比有明顯的增加(P≤0.05);而ME組與MI組肺重/體重比值(LW/BW)相比有明顯的降低(P≤0.01)。(3)ME的心肌組織IL-4分泌水平與MI組相比有明顯升高(P≤0.05);ME組心肌組織的IL-10分泌水平也顯著高于MI組(P≤0.05)。(4)Western blot 結(jié)果顯示,ME 組的 p-STAT6/t-STAT6 比值顯著高于MI 組(P≤0.05)。研究結(jié)論:(1)有氧運動有利于MI大鼠心臟舒縮功能的改善;(2)有氧運動有助于MI大鼠心功能的恢復,減輕炎癥反應;(3)有氧運動可以升高MI大鼠心肌組織中抑炎因子IL-4、IL-10的分泌水平;(4)有氧運動有助于激活大鼠心肌組織STAT6的磷酸化改變,從而抑制炎癥反應。
[Abstract]:Objective: to investigate the effects of aerobic exercise on the secretion of interleukin-4 (IL-4), interleukin-10 (IL-10) and phosphorylation of signal transduction protein and activator 6 (STAT6) in myocardial tissue after myocardial infarction (MI) in rats. And the mechanism of STAT 6 in inflammatory reaction. Methods: thirty male 8-week-old SD rats were randomly divided into sham-operated group, myocardial infarction group and myocardial infarction group. The MI model was established by left anterior descending branch ligation of coronary artery. Exercise program: 1 week of adaptive exercise followed by 10 m / min warm up for 10 minutes, then 20m/min for 8 minutes, then 15 m/min for 2 minutes, alternating 4 cycles, and finally 20 m/min for 8 minutes and 10 m/min for 2 min. Practice once a day for 5 d / w, and train for 8 ws. The myocardial wall thickness and ejection function were measured by two-dimensional echocardiography, the level of IL-4 IL-10 was detected by Elisa and the expression of p-STAT6t-STAT6 was detected by Western blot. Results compared with Sham group, the ventricular wall thinning and ejection function were decreased, and the ventricular wall thickness of MI rats was increased by aerobic exercise for 8 weeks (P 鈮，

本文編號：2030394