發(fā)布時(shí)間：2018-06-16 17:39

  本文選題：運(yùn)動(dòng)干預(yù) + 衰老性肌萎縮　； 參考：《武漢體育學(xué)院》2017年碩士論文

【摘要】：目的:通過(guò)自然飼養(yǎng)SD大鼠至21月齡構(gòu)建衰老性肌萎縮模型,采用兩種中等強(qiáng)度的運(yùn)動(dòng)干預(yù)方式(自由轉(zhuǎn)輪和爬梯運(yùn)動(dòng))探討運(yùn)動(dòng)干預(yù)調(diào)控蛋白質(zhì)降解通路對(duì)衰老性肌萎縮的改善作用,為衰老性肌萎縮的運(yùn)動(dòng)康復(fù)提供一定的理論依據(jù),為大眾健身提供一定的運(yùn)動(dòng)干預(yù)指導(dǎo)。方法:SD雄性大鼠19只飼養(yǎng)至21月齡用于構(gòu)建衰老性肌萎縮模型,將19只老年大鼠分為老年對(duì)照組(Senior Control Group,SC)6只,老年自由轉(zhuǎn)輪組(Senior Voluntary Wheel Running Group,SW)6只,老年爬梯組(Senior Ladder-Climbing Group,SL)7只;同時(shí),用10只3月齡成年大鼠作為年輕對(duì)照組(Youth Control Group,YC)。所有四組大鼠進(jìn)行運(yùn)動(dòng)干預(yù)8周后24小時(shí)內(nèi)稱取大鼠體重、眼球取血、收集腓腸肌并稱重。計(jì)算各組大鼠腓腸肌濕重與體重比值以評(píng)估肌萎縮程度,檢測(cè)血清超氧化物歧化酶(Superoxisome dismutase,SOD)、丙二醛(Malonaldehyde,MDA)的含量以比較各組大鼠抗氧化能力;TUNEL染色檢測(cè)腓腸肌細(xì)胞凋亡率;蛋白免疫印跡檢測(cè)骨骼肌能量代謝、蛋白降解和蛋白合成相關(guān)蛋白的表達(dá)。結(jié)果:1)與YC組相比,SC組大鼠腓腸肌濕重比顯著下降(p0.001);運(yùn)動(dòng)干預(yù)后SW組和SL組大鼠濕重比顯著性高于SC組(p0.05)。2)與YC組相比,SC組大鼠血清SOD顯著性下降(p0.001),MDA含量顯著性上升(p0.01);運(yùn)動(dòng)干預(yù)后SW組大鼠血清SOD顯著性的上升(p0.05),SL組雖然沒(méi)有顯著性差異但具有上升趨勢(shì)。3)HE染色結(jié)果顯示,與YC組相比,SC組大鼠腓腸肌肌纖維出現(xiàn)明顯的肌纖維萎縮和細(xì)胞核內(nèi)移的現(xiàn)象,運(yùn)動(dòng)干預(yù)后這種現(xiàn)象明顯得到改善;同時(shí)運(yùn)動(dòng)干預(yù)后SW和SL組大鼠腓腸肌纖維之間間隙減少,SL組肌纖維明顯增粗。4)TUNEL結(jié)果顯示,與YC組相比,SC組大鼠腓腸肌細(xì)胞凋亡率顯著性上升(p0.001),運(yùn)動(dòng)干預(yù)后老年大鼠腓腸肌細(xì)胞凋亡率都有明顯的下降(p0.01)。5)通過(guò)對(duì)各組大鼠腓腸肌中能量代謝相關(guān)蛋白表進(jìn)行檢測(cè),SC組大鼠腓腸肌中AMPK、Sirt1、PGC-1α表達(dá)量明顯下調(diào)(p0.01),運(yùn)動(dòng)干預(yù)后SW和SL組大鼠腓腸肌中能量代謝都有顯著性上升(p0.01)6)通過(guò)對(duì)各組大鼠腓腸肌中蛋白降解通路相關(guān)蛋白表達(dá)進(jìn)行檢測(cè),與YC組相比,SC組自噬水平明顯降低,LC3\Atg7\Beclin1表達(dá)量明顯下降(p0.01),而泛素蛋白酶標(biāo)志蛋白(Fox O3a/Atrogin1)表達(dá)顯著升高(p0.05),通過(guò)運(yùn)動(dòng)干預(yù)后自噬通路被激活,泛素蛋白酶通路相關(guān)蛋白表達(dá)下降。結(jié)論:1、衰老過(guò)程中伴隨著肌肉質(zhì)量的丟失,中等強(qiáng)度的運(yùn)動(dòng)干預(yù)對(duì)衰老性肌萎縮能夠起到一定的康復(fù)作用。2、衰老大鼠骨骼肌出現(xiàn)肌纖維橫截面積下降和細(xì)胞核內(nèi)移的現(xiàn)象。3、衰老大鼠體內(nèi)抗氧化酶含量下降,氧化損傷增加,通過(guò)運(yùn)動(dòng)干預(yù)后能夠顯著性提高骨骼肌細(xì)胞抗氧化能力。4、衰老大鼠骨骼肌細(xì)胞凋亡率增加,通過(guò)兩種不同形式運(yùn)動(dòng)干預(yù)(自由轉(zhuǎn)輪和爬梯運(yùn)動(dòng))都可以明顯減少老年大鼠的過(guò)度細(xì)胞凋亡。5、運(yùn)動(dòng)干預(yù)可以增加老年大鼠骨骼肌能量代謝,激活細(xì)胞自噬,減少泛素蛋白酶體調(diào)控的蛋白降解,增加蛋白質(zhì)的合成,對(duì)衰老性肌萎縮具有一定的康復(fù)作用。綜上所述,運(yùn)動(dòng)干預(yù)能夠通過(guò)調(diào)控蛋白質(zhì)降解的主要途徑,包括泛素蛋白酶體和自噬溶酶體,改善老年大鼠骨骼肌蛋白質(zhì)代謝失衡的狀態(tài)。本實(shí)驗(yàn)通過(guò)兩種中等強(qiáng)度運(yùn)動(dòng)干預(yù)調(diào)控蛋白質(zhì)降解改善衰老性肌萎縮,為老年人預(yù)防與治療衰老性肌萎縮提供了新的干預(yù)研究思路。
[Abstract]:Objective: to construct an aging Myoatrophy model by naturally feeding SD rats to 21 month old, and to explore the effect of exercise intervention on the improvement of aging muscular atrophy by two moderate intensity exercise interventions (free rotation wheel and climbing ladder movement), and provide a theoretical basis for the rehabilitation of aging Myoatrophy. General fitness provides certain exercise intervention guidance. Methods: 19 SD male rats were fed to 21 month old to construct an aging muscular atrophy model, and 19 old rats were divided into the elderly control group (Senior Control Group, SC), and the elderly free rotation group (Senior Voluntary Wheel Running Group, SW) 6, the elderly climbing ladder group (Senior) Ing Group, SL) 7; at the same time, 10 3 month old adult rats were used as the young control group (Youth Control Group, YC). All four groups of rats were weighed, the gastrocnemius muscle was collected and weighed in 24 hours after 8 weeks of exercise intervention. The ratio of gastrocnemius wet weight and weight was calculated to evaluate the degree of muscle atrophy and to detect serum super. Superoxisome dismutase (SOD), malondialdehyde (Malonaldehyde, MDA) content to compare the antioxidant capacity of rats in each group; TUNEL staining was used to detect the rate of gastrocnemius apoptosis; protein immunoblotting was used to detect skeletal muscle energy metabolism, protein degradation and protein synthase expression. Results: 1) compared with the YC group, the gastrocnemius of group SC rats The wet weight ratio of muscle decreased significantly (p0.001). After exercise intervention, the wet weight ratio of rats in group SW and SL group was higher than that in group SC (P0.05).2). Compared with group YC, the serum SOD significantly decreased (p0.001) and MDA content increased significantly (P0.01) in the SC group, and the group had no significant difference but had no significant difference after exercise intervention. Rising trend.3) HE staining results showed that the muscle fibers of the gastrocnemius muscle of the SC group showed obvious atrophy of muscle fibers and the movement of the nuclei in the group of the group of YC, and the phenomenon was obviously improved after the exercise. At the same time, the gap between the gastrocnemius fibers in the SW and SL groups was reduced and the SL group was significantly thickened with.4) in the SL group. Compared with group YC, the apoptosis rate of gastrocnemius cells in group SC was significantly increased (p0.001). The apoptosis rate of gastrocnemius cells in old rats after exercise intervention was significantly decreased (P0.01).5). The expression of AMPK, Sirt1 and PGC-1 alpha in gastrocnemius muscle of group SC was down significantly down (p0.). 01) after exercise intervention, the energy metabolism in the gastrocnemius muscle of SW and SL rats increased significantly (P0.01) 6) through the detection of protein degradation pathway related protein expression in the gastrocnemius muscle of rats. Compared with the YC group, the level of autophagy decreased significantly in the SC group, and the LC3Atg7Beclin1 expression decreased significantly (P0.01), and the ubiquitin protease marker protein (Fox O3a/A). Trogin1) significantly increased expression (P0.05), autophagy pathway was activated after exercise intervention, and the expression of ubiquitin protease pathway related protein decreased. Conclusion: 1, the loss of muscle mass in the aging process, moderate intensity exercise intervention for aging muscle atrophy can play a certain rehabilitation effect.2, the skeletal muscle fibers of aging rats appear muscle fiber The decline in cross section area and the phenomenon of cell nuclear migration.3, the antioxidant enzyme content in the aged rats decreased and the oxidative damage increased. After the exercise intervention, the antioxidant capacity of skeletal muscle cells could be significantly increased, the apoptosis rate of skeletal muscle cells in old rats increased, and two different forms of exercise intervention (free rotation wheel and ladder movement) were available. In order to reduce the excessive apoptosis.5 in old rats, exercise intervention can increase the energy metabolism of skeletal muscle in old rats, activate autophagy, reduce the protein degradation by ubiquitin proteasome, increase the synthesis of protein, and have a certain recovery for aging muscle atrophy. In summary, the exercise intervention can regulate the protein by regulating the protein. The main methods of qualitative degradation, including ubiquitin proteasome and autophagosome, improve the state of protein metabolism imbalance in skeletal muscles of old rats. This experiment provides new intervention research ideas for the prevention and treatment of aging muscle atrophy by regulating protein degradation by two kinds of moderate intensity exercise intervention.
【學(xué)位授予單位】：武漢體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：G804.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郭德華;殷曉旺;周群;劉春來(lái);;青少年網(wǎng)絡(luò)成癮的心理與運(yùn)動(dòng)干預(yù)研究[J];首都體育學(xué)院學(xué)報(bào);2007年06期

2 丁美紅,陳宜剛;運(yùn)動(dòng)干預(yù)對(duì)2型糖尿病治療效果的影響[J];泰州職業(yè)技術(shù)學(xué)院學(xué)報(bào);2005年04期

3 宋哲明;李蕩;宋健;;中老年2型糖尿病患者運(yùn)動(dòng)干預(yù)模型的研究[J];吉林體育學(xué)院學(xué)報(bào);2008年03期

4 段峰;;跌倒預(yù)防運(yùn)動(dòng)干預(yù)對(duì)社區(qū)老年人運(yùn)動(dòng)能力的影響[J];山東體育學(xué)院學(xué)報(bào);2011年11期

5 蘇龍;席莉;黃武勝;;基于高校平臺(tái)的有組織運(yùn)動(dòng)干預(yù)對(duì)肥胖男性大學(xué)生生理指標(biāo)的影響研究[J];吉林體育學(xué)院學(xué)報(bào);2011年03期

6 李建平;;脂聯(lián)素對(duì)運(yùn)動(dòng)干預(yù)2型糖尿病的反應(yīng)及其可能機(jī)制[J];嘉興學(xué)院學(xué)報(bào);2010年03期

7 李佳川;;網(wǎng)絡(luò)成癮問(wèn)題與運(yùn)動(dòng)干預(yù)探究[J];體育科技文獻(xiàn)通報(bào);2012年07期

8 袁紹輝;;高職院校學(xué)生體質(zhì)健康運(yùn)動(dòng)干預(yù)研究[J];青春歲月;2012年12期

9 秦素榮;王志英;;老年人跌倒的生理學(xué)因素分析及運(yùn)動(dòng)干預(yù)[J];當(dāng)代體育科技;2012年11期

10 張超;劉東升;單猛;王楓林;彭坤;;醫(yī)學(xué)院校網(wǎng)絡(luò)成癮大學(xué)生運(yùn)動(dòng)干預(yù)效果研究[J];中國(guó)高等醫(yī)學(xué)教育;2013年02期

相關(guān)會(huì)議論文 前10條

1 王秀景;;飲食運(yùn)動(dòng)干預(yù)治療兒童單純性肥胖癥[A];2008年浙江省內(nèi)分泌學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2008年

2 吳華;吳紀(jì)饒;;對(duì)絕經(jīng)后女子骨質(zhì)疏松的運(yùn)動(dòng)干預(yù)研究[A];第七屆全國(guó)體育科學(xué)大會(huì)論文摘要匯編（二）[C];2004年

3 姜雪;常翠青;黃志卓;劉文;趙小倩;陳志民;陳燕波;;Plin基因11482 G＞A單核苷酸多態(tài)性與運(yùn)動(dòng)干預(yù)肥胖漢族少年減肥效果的相關(guān)關(guān)系研究[A];中國(guó)營(yíng)養(yǎng)學(xué)會(huì)第十次全國(guó)營(yíng)養(yǎng)學(xué)術(shù)會(huì)議暨第七屆會(huì)員代表大會(huì)論文摘要匯編[C];2008年

4 曹舒奮;;運(yùn)動(dòng)干預(yù)對(duì)上海市民心理健康狀況影響的研究[A];全民健身科學(xué)大會(huì)論文摘要集[C];2009年

5 姜雪;常翠青;黃志卓;劉文;趙小倩;陳志民;陳燕波;;Plin基因11482 G>A單核苷酸多態(tài)性與運(yùn)動(dòng)干預(yù)肥胖漢族少年減肥效果的相關(guān)關(guān)系研究[A];中國(guó)營(yíng)養(yǎng)學(xué)會(huì)第十次全國(guó)營(yíng)養(yǎng)學(xué)術(shù)會(huì)議公共營(yíng)養(yǎng)分會(huì)論文集[C];2008年

6 馮鵬程;楊寶國(guó);余永章;;運(yùn)動(dòng)干預(yù)在戒毒矯治中的應(yīng)用研究[A];中國(guó)康復(fù)醫(yī)學(xué)會(huì)運(yùn)動(dòng)療法分會(huì)第十一屆全國(guó)康復(fù)學(xué)術(shù)大會(huì)學(xué)術(shù)會(huì)議論文摘要匯編[C];2011年

7 董貴俊;李驍君;胡斌;李可峰;;不同組合干預(yù)方法對(duì)單純性肥胖青少年血壓、血脂等的影響[A];第八屆全國(guó)體育科學(xué)大會(huì)論文摘要匯編（一）[C];2007年

8 唐東輝;劉潤(rùn)芝;高倩;;網(wǎng)膜素在運(yùn)動(dòng)干預(yù)改善肥胖者內(nèi)皮功能中的作用研究進(jìn)展[A];2013年中國(guó)生理學(xué)會(huì)運(yùn)動(dòng)生理學(xué)專業(yè)委員會(huì)年會(huì)暨“運(yùn)動(dòng)與健康”學(xué)術(shù)研討會(huì)論文摘要匯編[C];2013年

9 李春艷;;運(yùn)動(dòng)干預(yù)對(duì)心力衰竭患者運(yùn)動(dòng)能力的影響[A];2013年中國(guó)生理學(xué)會(huì)運(yùn)動(dòng)生理學(xué)專業(yè)委員會(huì)年會(huì)暨“運(yùn)動(dòng)與健康”學(xué)術(shù)研討會(huì)論文摘要匯編[C];2013年

10 李春艷;;運(yùn)動(dòng)干預(yù)對(duì)心力衰竭患者運(yùn)動(dòng)能力的影響[A];2013年中國(guó)生理學(xué)會(huì)運(yùn)動(dòng)生理學(xué)專業(yè)委員會(huì)年會(huì)暨“運(yùn)動(dòng)與健康”學(xué)術(shù)研討會(huì)論文摘要匯編[C];2013年

相關(guān)重要報(bào)紙文章 前1條

1 衣曉峰邋靳萬(wàn)慶 李華妍;飲食和運(yùn)動(dòng)干預(yù)可預(yù)防或推遲糖尿病的發(fā)生[N];中國(guó)中醫(yī)藥報(bào);2008年

相關(guān)博士學(xué)位論文 前4條

1 常鳳;2型糖尿病運(yùn)動(dòng)干預(yù)方案及策略研究[D];武漢體育學(xué)院;2015年

2 石勱;老年2型糖尿病患者運(yùn)動(dòng)干預(yù)效果的系統(tǒng)評(píng)價(jià)與實(shí)證研究[D];北京中醫(yī)藥大學(xué);2016年

3 汪毅;55-65歲靜坐少動(dòng)女性運(yùn)動(dòng)干預(yù)的時(shí)效性研究[D];北京體育大學(xué);2016年

4 吳煒煒;運(yùn)動(dòng)干預(yù)改善虛弱前期老人睡眠與虛弱狀況的研究[D];福建醫(yī)科大學(xué);2016年

相關(guān)碩士學(xué)位論文 前10條

1 白義松;基于能量代謝的視角探討運(yùn)動(dòng)干預(yù)對(duì)肥胖大學(xué)生體成分影響的研究[D];西南交通大學(xué);2015年

2 張茜茜;大課間活動(dòng)對(duì)肥胖群體學(xué)生運(yùn)動(dòng)干預(yù)的實(shí)驗(yàn)研究[D];天津體育學(xué)院;2015年

3 張琛琛;運(yùn)動(dòng)干預(yù)對(duì)肥胖青少年影響的Meta分析及對(duì)學(xué)校體育的啟示[D];上海體育學(xué)院;2015年

4 劉通;運(yùn)動(dòng)干預(yù)對(duì)女大學(xué)生健康體適能影響的研究[D];哈爾濱師范大學(xué);2015年

5 童霄娟;非酒精性脂肪肝病的飲食、運(yùn)動(dòng)干預(yù)效果分析[D];浙江中醫(yī)藥大學(xué);2016年

6 彭世軍;單純性肥胖青少年運(yùn)動(dòng)干預(yù)效果的研究[D];西安體育學(xué)院;2016年

7 何甜甜;運(yùn)動(dòng)干預(yù)對(duì)大學(xué)生焦慮癥狀的效應(yīng)檢驗(yàn)[D];曲阜師范大學(xué);2016年

8 尉迎麗;制定運(yùn)動(dòng)處方針對(duì)慢性非特異性下腰痛“骨盆前傾—腰曲增加”型功能異常的運(yùn)動(dòng)干預(yù)研究[D];成都體育學(xué)院;2016年

9 謝yN;注意缺陷多動(dòng)障礙兒童的運(yùn)動(dòng)干預(yù)方案設(shè)計(jì)與個(gè)案研究[D];南京體育學(xué)院;2015年

10 肇啟瞳;超重初中生體質(zhì)調(diào)查及運(yùn)動(dòng)干預(yù)研究[D];遼寧師范大學(xué);2016年

，

本文編號(hào)：2027559