發(fā)布時間：2018-04-30 10:01

  本文選題：有氧運動 + 肥胖　； 參考：《武漢體育學(xué)院學(xué)報》2017年07期

【摘要】：目的:觀察有氧運動對大鼠肝臟功能以及CYP7A1mRNA的表達。方法:對高脂飲食飼養(yǎng)的肥胖及肥胖抵抗大鼠進行8周的運動干預(yù),用組織學(xué)染色及血清生化檢測,評價大鼠肝臟脂代謝及功能;用相對定量PCR評價CYP7A1mRNA的表達情況。結(jié)果:肥胖對照組大鼠HE染色肝細胞胞質(zhì)內(nèi)顯現(xiàn)大小不等空泡,肝竇間隙明顯變窄,肥胖游泳組肝細胞形態(tài)基本正常,細胞核居中,肝細胞脂肪變性明顯減輕,肝竇間隙無明顯變化;肥胖對照組、肥胖抵抗對照組大鼠血清ALT、AST水平高于普飼對照組并且具有非常顯著的差異,肥胖游泳組大鼠血清ALT、AST顯著低于肥胖對照組;肥胖對照組大鼠CYP7A1mRNA相對表達量高度顯著低于普飼對照組。肥胖游泳組大鼠CYP7A1mRNA相對表達量顯著高于肥胖對照組。結(jié)論:10周高脂飲食可引肥胖及肥胖抵抗大鼠血脂出現(xiàn)異常,脂質(zhì)代謝紊亂、肝臟出現(xiàn)脂肪沉積、肝功能異常;有氧運動可能通過增加肥胖大鼠肝臟CYP7A1表達,改善膽固醇轉(zhuǎn)化障礙,從而達到改善10周高脂飲食導(dǎo)致的肝功能異常的現(xiàn)象。
[Abstract]:Objective: to observe the effect of aerobic exercise on liver function and CYP7A1mRNA expression in rats. Methods: eight weeks exercise intervention was performed on obese and obese resistant rats fed with high fat diet. The liver lipid metabolism and function were evaluated by histological staining and serum biochemical test, and the expression of CYP7A1mRNA was evaluated by relative quantitative PCR. Results: in the obese control group, different vacuoles appeared in the cytoplasm of hepatocytes stained with HE, and the hepatic sinusoidal space was narrowed obviously. In the obese swimming group, the hepatocyte morphology was normal, the nucleus was in the middle, and the steatosis of the hepatocytes was alleviated. There was no significant change in the hepatic sinusoidal space, the serum alt AST level in obese control group and obese resistance control group was higher than that in general feeding control group and there was a significant difference, and the serum alt level in obese swimming group was significantly lower than that in obese control group. The relative expression of CYP7A1mRNA in obese control group was significantly lower than that in general feeding control group. The relative expression of CYP7A1mRNA in obese swimming group was significantly higher than that in obese control group. Conclusion 10 weeks of high fat diet can induce abnormal blood lipids, abnormal lipid metabolism, fat deposition and abnormal liver function in obese and obese resistant rats, aerobic exercise may increase the expression of CYP7A1 in the liver of obese rats. Improve cholesterol conversion disorder to improve liver dysfunction caused by 10-week high-fat diet.
【作者單位】： 華中科技大學(xué)同濟醫(yī)學(xué)院附屬武漢兒童醫(yī)院康復(fù)醫(yī)學(xué)科;江西省體育科學(xué)研究所;武漢體育學(xué)院健康科學(xué)學(xué)院;
【基金】：國家體育總局科研課題(2015B089) 霍英東教育基金會青年教師基金基礎(chǔ)性研究課題(121103)
【分類號】：G804.2

【參考文獻】

相關(guān)期刊論文 前7條

1 連軍;馬琪;霍新慧;呂曉敏;;高脂飲物快速建立營養(yǎng)性肥胖動物模型的研究[J];新疆醫(yī)科大學(xué)學(xué)報;2008年10期

2 李玉中;王朝暉;應(yīng)力;;復(fù)方甘正浸膏對非酒精性脂肪性肝炎CYP2E1和PPARα表達的影響[J];中國中醫(yī)急癥;2008年08期

3 金麗;吳峻;汪軍;;肥胖以及有氧耐力運動對大鼠血清抗氧化酶和一氧化氮的影響[J];華中師范大學(xué)學(xué)報(自然科學(xué)版);2007年04期

4 楊英昕;朱培庭;張靜U，

本文編號：1824108