本文選題：骨骼肌鈍挫傷　切入點(diǎn)：損傷修復(fù)　出處：《上海體育學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過觀察小鼠骨骼肌挫傷修復(fù)過程中肌再生因子、炎癥因子以及相關(guān)趨化因子的表達(dá)規(guī)律,探討肌再生因子、炎癥因子以及相關(guān)趨化因子在骨骼肌挫傷修復(fù)過程中的表達(dá)規(guī)律以及可能發(fā)揮的作用。方法:共使用40只雄性C57小鼠,隨機(jī)選擇8只作為對照組(C組,n=8),其余小鼠進(jìn)行骨骼肌鈍挫傷(S組,n=32),損傷后分別在1,3,7,14進(jìn)行小鼠腓腸肌取材。小鼠右側(cè)腓腸肌制作石蠟標(biāo)本,用于HE染色法顯示骨骼肌損傷以及損傷修復(fù)的骨骼肌組織形態(tài)結(jié)構(gòu),左側(cè)腓腸肌用于基因表達(dá)檢測,用熒光定量PCR檢測肌再生調(diào)節(jié)因子(MyoD,myogenin,IGF-1,MGF,HGF,TGF-β1,Myostain),炎癥因子(IL-1β,IL-6,IL-10)以及趨化因子(CCL2,CCL3,CCL5,CCL8,CXCL9,CXCL10,CXCL12)mRNA 表達(dá)變化。結(jié)果:1、骨骼肌鈍挫傷后第3天有少量再生肌纖維,第7天顯著增加,第14天基本恢復(fù)正常。2、MyoD和Myogenin分別是肌衛(wèi)星細(xì)胞增殖和分化特異性標(biāo)志物,在骨骼肌鈍挫傷后顯著表達(dá)(P0.01)。3、M1型巨噬細(xì)胞標(biāo)志物CD68 mRNA在骨骼肌損傷的第1天和第3天顯著增加(p0.01),M2型巨噬細(xì)胞標(biāo)志物CD163和CD206 mRNA都在骨骼肌損傷的第1天顯著增加(p0.01),之后CD163 mRNA迅速下降,而CD206 mRNA在骨骼肌損傷后第三天繼續(xù)增加(p0.01)4、與對照組相比,生肌調(diào)節(jié)因子(IGF-1,MGF,HGF,TGF-β 1)mRNA在損傷后表達(dá)顯著增加(P0.05或P0.01),MSTNmRNA在損傷后再生階段的第7天到第14天,MSTN mRNA表達(dá)顯著低于對照組(P0.01)。5、炎性細(xì)胞因子(IL-1β,IL-6,IL-10)mRNA 在骨骼肌損傷后顯著性表達(dá)(p0.01或P0.05)6、趨化因子(CCL2,CCL3,CCL5,CCL8,CXCL9,CXCL10,CXCL12)mRNA 在骨骼肌鈍挫傷后顯著性表達(dá),其中CCL2 mRNA在骨骼肌損傷后第1天已經(jīng)開始顯著性升高(P0.01),CCL3,CCL5與CCL8 mRNA在骨骼肌損傷后第1天顯著性增加(p0.01),第7天與第3天相比,mRNA水平雖有下降,但與對照組相比,仍顯著增加(p0.01)。CXCL9,CXCL12 mRNA均在第7天顯著性增加(p0.01),隨后下降。其中CXCL10 mRNA在損傷后第一天呈增加趨勢,損傷后第3天與對照組相比呈顯著性差異,這種增加趨勢直到損傷后第7天后才開始下降。結(jié)論:1、骨骼肌挫傷后,M1、M2型巨噬細(xì)胞標(biāo)志物及多種炎性因子(IL-1β,IL-6,IL-10)表達(dá)上調(diào),它們可能在骨骼肌損傷修復(fù)過程中發(fā)揮重要作用。2、趨化因子CCL2,CXCL10可能參與了骨骼肌挫傷修復(fù)階段免疫細(xì)胞的趨化。3、骨骼肌挫傷修復(fù)過程中,IGF-1,MGF,HGF,TGF-β1,Myostain等肌再生調(diào)控因子可能參與了對骨骼肌再生的調(diào)控。
[Abstract]:Objective: to investigate the expression of muscle regeneration factors, inflammatory factors and related chemokines during the repair of skeletal muscle contusion in mice. Expression of inflammatory factors and related chemokines in skeletal muscle contusion repair and their possible role. Methods: 40 male C57 mice were used. Eight mice were randomly selected as control group C, and the rest mice were treated with skeletal muscle contusion group S, and then the gastrocnemius muscle samples were taken from the gastrocnemius muscle at 1: 3 and 714, respectively. Paraffin wax specimens were made from the right gastrocnemius muscle of the mice. The left gastrocnemius muscle was used to detect gene expression. Fluorescence quantitative PCR was used to detect the mRNA expression of myogenic regulatory factor MyoDmyogenin IGF-1MGF- 尾 1 (Myostaina), the inflammatory factor IL-1 尾 -IL-6 IL-10) and the chemokine CCL2CCL5CCL5CCL8CXCL9CXCL10CXCL1212. Results: there was a small amount of regenerated muscle fibers on the 3rd day after blunt contusion of skeletal muscle, which was significantly increased on the 7th day. On the 14th day, MyoD and Myogenin were respectively specific markers for proliferation and differentiation of myosatellous cells. After skeletal muscle contusion, the expression of CD68 mRNA, a type M 1 macrophage marker, was significantly increased on the 1st and 3rd day after skeletal muscle injury. Both CD163 and CD206 mRNA were significantly increased on the first day of skeletal muscle injury, and the expression of CD206 mRNA increased significantly on the first day of skeletal muscle injury. Then CD163 mRNA dropped rapidly, However, CD206 mRNA continued to increase on the third day after skeletal muscle injury, compared with the control group. The expression of MSTN mRNA of IGF-1 / MGF- HGF- 尾 _ (1) increased significantly after injury. The expression of MSTN mRNA was significantly lower than that of control group from 7th to 14th day after injury, and the expression of inflammatory cytokine IL-1 尾 IL-6IL-10 mRNA was significantly higher than that of control group (P 0.01 or P 0.01 or P 0.01 or P 0.01 or P 0.01) after the injury of the skeletal muscle. The expression of MSTN mRNA was significantly lower than that of the control group from day 7 to day 14. The expression of the inflammatory cytokine IL-1 尾 IL-6mGF- 尾 IL-10 mRNA was significantly higher than that of the control group (P 0.01 or P 0.01). The expression of CXCL10, CXCL10, CXCL12 mRNA in skeletal muscle after blunt contusion was significant. CCL2 mRNA began to increase significantly on the first day after skeletal muscle injury. P0.01CCL5 and CCL8 mRNA increased p0.01a on the 1st day after skeletal muscle injury, and decreased on the 7th day compared with the 3rd day, but compared with the control group. The mRNA of CXCL9 + CXCL12 was significantly increased on the 7th day, and then decreased. The CXCL10 mRNA increased on the first day after injury, and on the third day after injury, there was a significant difference compared with the control group. This increased trend did not begin to decrease until 7 days after injury. Conclusion the expression of M1M2-type macrophage markers and various inflammatory cytokines (IL-1 尾 / IL-6 / IL-10) is up-regulated after skeletal muscle contusion. They may play an important role in the repair of skeletal muscle injury. The chemokine CCL2CXCL10 may be involved in the chemotaxis of immune cells in the repair stage of skeletal muscle contusion. IGF-1, MGFHGF- 尾 1, Myostain and other muscle regeneration regulatory factors may be involved in the repair of skeletal muscle contusion. Participate in the regulation of skeletal muscle regeneration.
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.2

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