本文選題：微壓氧　切入點：大強度訓(xùn)練　出處：《上海體育學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:本研究的目的是圍繞氧化應(yīng)激和毛細血管再生,從自由基的產(chǎn)生和清除以及毛細血管再生相關(guān)因子的基因和蛋白表達調(diào)控探討微壓氧對于大強度訓(xùn)練后的恢復(fù)機制,為微壓氧在運動訓(xùn)練后恢復(fù)中的應(yīng)用提供理論依據(jù)和實驗支持。研究方法:本研究選用SPF級8周齡雄性SD大鼠40只,經(jīng)一周的適應(yīng)性訓(xùn)練后篩選出30只,隨機分為對照組(C組)、大強度訓(xùn)練后安靜恢復(fù)組(HQ組,26.8m/min,10°)和微壓氧恢復(fù)組(HM組),每組10只。本實驗采用的微壓氧倉氧濃度為26%左右,壓強為3.6PSI。訓(xùn)練周期為8周,每周訓(xùn)練5天,每天1小時。HM組每次訓(xùn)練結(jié)束后即刻放入微壓氧倉恢復(fù)1小時。在第8周最后一次訓(xùn)練結(jié)束24小時后進行麻醉,切取左側(cè)腓腸肌用于氧化應(yīng)激指標的檢測和觀察腓腸肌組織形態(tài);采用實時熒光定量PCR、Western blot技術(shù)檢測大鼠腓腸肌VEGF、VEGFR2、b FGF基因和蛋白水平的變化。數(shù)據(jù)應(yīng)用SPSS19.0統(tǒng)計軟件進行處理,以P0.05認為有顯著性差異。研究結(jié)果:1.大鼠力竭時間的變化:HM組大鼠跑至力竭時間顯著長于HQ組(P0.05)。2.氧化應(yīng)激指標的變化:HQ組肌肉MDA含量高于C組和HM組,具有顯著性差異(P0.05);HM組肌肉SOD活性顯著性高于C組(P0.05);HM組肌肉GSH-PX的活性高于C組和HQ組,并具有顯著性差異(P0.05)。3.骨骼肌組織形態(tài)學(xué)改變:與C組相比,HQ組大鼠和HM組大鼠骨骼肌損傷,但是HM組輕于HQ組。4.微壓氧對毛細血管密度及毛細血管再生相關(guān)因子表達的影響:(1)毛細血管再生相關(guān)因子表達:HQ組與C組和HM組相比VEGF蛋白相對表達升高,具有顯著性差異(P0.05);HQ組VEGFR2m RNA表達顯著性高于HM組(P0.05);VEGFR2蛋白的相對表達HQ組顯著性高于C組和HM組(P0.05);b FGFm RNA HM組低于HQ組,具有顯著性差異(P0.05);HQ組與C組和HM組相比,b FGF蛋白相對表達量增加,且差異具有統(tǒng)計學(xué)意義(P0.05)。(2)毛細血管密度:HM組與C組相比毛細血管密度增加,具有極顯著性差異(P0.01);HM組與HQ組相比,毛細血管密度增加,具有顯著性差異(P0.05);HQ組毛細血管密度顯著高于C組(P0.05)。研究結(jié)論:1.大強度訓(xùn)練可造成大鼠骨骼肌氧化應(yīng)激損傷,使其運動能力下降;而微壓氧可提高骨骼肌抗氧化酶活性,減少骨骼肌脂質(zhì)過氧化反應(yīng),提高運動能力;2.大強度訓(xùn)練可以引起骨骼肌毛細血管密度增加,而微壓氧可加速新生毛細血管的生成,從而可能促進氧、營養(yǎng)物質(zhì)以及代謝產(chǎn)物的運輸,加速運動后的恢復(fù)。
[Abstract]:Objective: the aim of this study was to investigate the recovery mechanism of microbaric oxygen after intensive training by focusing on oxidative stress and capillary regeneration, from the production and clearance of free radicals and the regulation of gene and protein expression of factors related to capillary regeneration. To provide theoretical basis and experimental support for the application of microbaric oxygen in the recovery of exercise training. Methods: 40 male SD rats of SPF grade 8 weeks of age were selected and 30 rats were selected after one week of adaptive training. The rats were randomly divided into control group (C group), quiet recovery group (HQ group) (26.8m / min 10 擄) and micro-pressure oxygen recovery group (HM group) (10 rats in each group). The oxygen concentration and pressure of micro-pressure oxygen chamber were about 26% and 3.6 PSI.The training period was 8 weeks and the training period was 5 days per week. One hour per day. The HM group was put into the oxygen chamber for 1 hour immediately after each training, and anesthetized 24 hours after the last training in the 8th week. The left gastrocnemius muscle was removed to detect the index of oxidative stress and the tissue morphology of gastrocnemius muscle was observed. The changes of VEGFN VEGFR2b FGF gene and protein in gastrocnemius muscle of rats were detected by real-time fluorescence quantitative PCR blot. The data were processed by SPSS19.0 software. The results showed that there was significant difference between the two groups. The results showed that the time of exhaustion was significantly longer in the group of rats than in the group HQ. The changes of oxidative stress index were higher in the group of oxidative stress than in the group C and HM, the content of MDA in the muscle of the group was higher than that in the group C and HM, and the change of the index of oxidative stress was higher than that of the group of C and HM. There was significant difference in muscle SOD activity in P0.05HM group than that in C group P0.05HM group, and there was significant difference in muscle GSH-PX activity between group C and HQ group. The histomorphologic changes of skeletal muscle: compared with group C and HM group, skeletal muscle injury in HM group and HM group was significant higher than that in P0.05 HM group, and there was a significant difference in muscle GSH-PX activity between HM group and HM group, compared with group C, the muscle GSH-PX activity in HM group was significantly higher than that in group C (P 0.05). However, HM group was lighter than HQ group .4.The effect of microbaric oxygen on the expression of capillary density and capillary regeneration related factors: 1) the expression of capillary regeneration related factor in group 1: HQ was higher than that in group C and HM, and the expression of VEGF protein in group 1 was higher than that in group C and HM. The expression of VEGFR2m RNA in HM group was significantly higher than that in HM group. The expression of VEGFR2 protein in HM group was significantly higher than that in C group and HM group. The expression of VEGFR2 protein in HM group was significantly higher than that in HM group, and the expression of VEGFR2 protein in HM group was significantly higher than that in HM group (P 0.05 FGFm RNA HM group). There was significant difference in the relative expression of FGF protein between P0.05HM group and C group and HM group, and the difference was statistically significant (P 0.05. 05) the capillary density of HM group was higher than that of C group, and the capillary density of HM group was higher than that of C group. The capillary density of HM group was significantly higher than that of HQ group, and the capillary density of HM group was significantly higher than that of C group. Micropressure oxygen can increase the activity of antioxidant enzymes, reduce lipid peroxidation, and improve the exercise ability of skeletal muscle. High intensity training can increase the density of capillaries in skeletal muscle. Microbaric oxygen can accelerate the formation of new capillaries, which may promote the transport of oxygen, nutrients and metabolites, and accelerate the recovery after exercise.
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.2

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