本文關(guān)鍵詞： 甲烷生理鹽水 氧化應(yīng)激 一次性力竭運動　出處：《上海體育學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:探究運動前后腹腔注射甲烷生理鹽水對雄性SD大鼠一次性力竭運動后氧化應(yīng)激損傷的影響,為甲烷生理鹽水在運動科學(xué)領(lǐng)域的應(yīng)用提供實驗依據(jù)。研究方法:21只清潔級健康雄性SD大鼠,2月齡,體重200~250g,適應(yīng)性喂養(yǎng)1周。按體重配對隨機分為運動安慰劑組(TP)、甲烷水恢復(fù)組(TM)、甲烷水預(yù)防恢復(fù)組(PTM),每組7只。三組大鼠進行10min速度為15m/min的適應(yīng)性跑臺訓(xùn)練,休息兩天后,PTM組大鼠于運動前5min按10ml/kg體重的劑量腹腔注射甲烷生理鹽水,TP、TM組大鼠以相同的劑量和方法給予生理鹽水干預(yù)。三組同時進行跑臺坡度為0°,速度為35m/min的一次性力竭跑臺運動。判定力竭后記錄其運動時間,并即刻給予TM、PTM組按10ml/kg體重的劑量腹腔注射甲烷生理鹽水,TP組大鼠以相同的劑量和方法給予生理鹽水干預(yù)。力竭運動結(jié)束后10min和3h分別于眼內(nèi)眥和腹主靜脈處取血,檢測血常規(guī)、血糖及乳酸濃度,其余全血于肝素鈉抗凝管中混勻,分離血漿,檢測血中尿素氮、總抗氧化物濃度和肌酸激酶、谷丙轉(zhuǎn)氨酶、谷草轉(zhuǎn)氨酶、超氧化物歧化酶、髓過氧化物酶活性。所有實驗數(shù)據(jù)均采用SPSS17.0軟件進行處理和分析。實驗結(jié)果以p0.05表示差異具有統(tǒng)計學(xué)意義。研究結(jié)果:(1)與TP組、TM組比較,PTM組大鼠的運動時間顯著延長(*p0.05,#p0.05)；(2)與TP組相比,TM組在運動結(jié)束后10min,BPC、PCT升高(**p0.01)；在運動結(jié)束后3h,RBC升高(*p0.05)。與TM組相比,PTM組在運動結(jié)束后10min,WBC、Hb降低(#p0.05),在運動結(jié)束后3h,WBC、RBC、HCT、Hb均降低；(3)運動結(jié)束后10min和3h時,三組大鼠的血糖、血乳酸、尿素氮均無統(tǒng)計學(xué)差異。TM組大鼠血肌酸激酶與TP組、PTM組相比,在運動結(jié)束后10min時無差異,運動結(jié)束后3h顯著升高(*p0.05,##p0.01)；(4)三組大鼠的血谷丙轉(zhuǎn)氨酶在運動結(jié)束后10min和3h時均無統(tǒng)計學(xué)差異,TM組大鼠血谷草轉(zhuǎn)氨酶與TP組、PTM組相比,在運動結(jié)束后10min時無差異,運動結(jié)束后3h顯著升高(*p0.05,#p0.05)；(5)TM組大鼠血髓過氧化物酶在運動結(jié)束后10min時顯著高于TP組(**p0.01),在運動結(jié)束后3h時均顯著高于TP組、PTM組(**p0.01,##p0.01)；(6)在運動結(jié)束后10min時,PTM組大鼠丙二醛、超氧化物歧化酶和總抗氧化物均顯著低于TP組、TM組(*p0.05,#p0.05)；在運動結(jié)束后3h時,TM組大鼠與TP組相比超氧化物歧化酶顯著升高(**p0.01)、總抗氧化物有升高的趨勢,PTM組大鼠超氧化物歧化酶顯著高于TP組(**p0.01),總抗氧化物顯著高于TP組和TM組(**p0.01,#p0.05)。研究結(jié)論:(1)運動前腹腔注射甲烷生理鹽水可以顯著延長雄性SD大鼠運動至力竭的運動時間。(2)運動后腹腔注射甲烷生理鹽水可能影響了細(xì)胞膜功能的恢復(fù),在一定程度上降低了大鼠的抗氧化能力。(3)運動前后腹腔注射甲烷生理鹽水在一定程度上也降低了機體的總抗氧化能力。
[Abstract]:Objective: to investigate the effects of intraperitoneal injection of methane-saline on oxidative stress injury in male SD rats after exhaustive exercise before and after exercise. To provide experimental basis for the application of methane-saline in the field of sports science. Methods: twenty one healthy male SD rats of clean grade were 2 months old. The rats were randomly divided into the exercise placebo group, the methane water recovery group and the methane water recovery group, with 7 rats in each group. The rats in the three groups were trained on an adaptive platform with a speed of 15 m / min for 10 minutes. After two days of rest, rats in PTM group were injected intraperitoneally with 10 ml / kg body weight of 10 ml / kg body weight at the dose of 10 ml / kg body weight and treated with the same dose and method of normal saline at 5 min before exercise. The three groups were treated with the same dose and method of normal saline, and the three groups were treated with a running platform slope of 0 擄and a speed of 35 m / min. One time exhaustive treadmill movement. After exhaustion, record the time of movement, The rats in the TMN PTM group were given 10 ml / kg body weight intraperitoneal injection of methane-saline and TP group with the same dose and method. Blood samples were taken from the medial canthus and the abdominal main vein at 10 min and 3 h after exhaustive exercise, and the blood routine was detected. Blood glucose and lactate concentration were mixed in heparin sodium anticoagulant tube, plasma was separated, blood urea nitrogen, total antioxidant concentration and creatine kinase, alanine aminotransferase, glutamic oxalacetic transaminase, superoxide dismutase were detected. Myeloperoxidase activity. All experimental data were processed and analyzed by SPSS17.0 software. The results showed significant difference in p0.05. The results of the study showed that the exercise time of rats in TP group was significantly higher than that in TP group (P < 0. 05). Compared with TP group, compared with TP group, compared with TP group, the increase of BPC-PCT in TM group was significantly higher than that in TP group 10 min after exercise, and the RBC increased at 3 h after exercise. Compared with TM group, 10 mins after exercise, 10 mins after exercise and 3 h after exercise, the levels of BBCCHb in PTM group were lower than those in TP group, and the levels of HCTHb were decreased at 10min and 3h after exercise. There was no significant difference in blood glucose, blood lactic acid and urea nitrogen among the three groups. There was no difference in creatine kinase between TM group and TP group at 10 min after exercise. There was no significant difference in serum alanine aminotransferase between TM group and TP group at 10 min and 3 h after exercise. There was no significant difference in serum alanine aminotransferase between TM group and TP group at 10 min after exercise. 3 h after exercise, there was a significant increase in serum myeloperoxidase (myeloperoxidase) in the TM group than that in the TP group at 10 min after exercise, and was significantly higher than that in the TP group at 3 h after the end of exercise. At 3 h after exercise, it was significantly higher than that in the TP PTM group and ##p0.01D group) at 10 min after the end of exercise, malondialdehyde (MDA) in PTM group was significantly higher than that in the PTM group (P < 0.05), and the level of malondialdehyde in the PTM group was significantly higher than that in the PTM group at 3 h after the end of exercise. Superoxide dismutase (SOD) and total antioxidants were significantly lower than those in TP group (TM group) and #p0.05G group (P < 0.05), and at 3 h after exercise in TM group compared with TP group, superoxide dismutase (SOD) in TM group was significantly higher than that in TP group (P 0.01), and total antioxidants in PTM group had a tendency to increase superoxidation. The activity of dismutase was significantly higher than that of TP group, and the total antioxidant content was significantly higher than that of TP group and TM group. Conclusion the intraperitoneal injection of normal saline can significantly prolong the exercise time from exercise to exhaustion in male Sprague-Dawley rats before exercise. Injection of normal saline into the cavity may affect the recovery of cell membrane function. To a certain extent, the anti-oxidation ability of rats was decreased by intraperitoneal injection of methane-saline before and after exercise, and the total antioxidant capacity was also decreased to a certain extent.
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.2

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