本文選題：肺痿 + 高效液相色譜指紋圖譜��； 參考：《北京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：專業(yè)學(xué)位是培養(yǎng)特定職業(yè)高層次創(chuàng)新應(yīng)用型專門人才的重要途徑,近年來成為我國研究生教育的重要形式。而中醫(yī)中藥作為我國獨特的醫(yī)藥學(xué)體系和國學(xué)文化的延伸,已經(jīng)受到國家的高度重視,國家對中醫(yī)藥學(xué)科專業(yè)應(yīng)用型人才的需求日益增加。因此,國務(wù)院學(xué)位委員會決定設(shè)置中藥學(xué)碩士專業(yè)學(xué)位,并于2011年起我國首次招收中藥學(xué)專業(yè)學(xué)位研究生。目的是培養(yǎng)具有實踐能力和創(chuàng)新應(yīng)用能力的高層次復(fù)合人才,以適應(yīng)我國中藥行業(yè)現(xiàn)代化,多元化的發(fā)展模式。中藥學(xué)碩士專業(yè)學(xué)位是一種有別于學(xué)術(shù)型碩士的高級人才培養(yǎng)模式。專業(yè)學(xué)位采用"校企合作"、"政、校、行、企"合作的"雙導(dǎo)師制"的培養(yǎng)模式,理論課程以學(xué)校為主,實踐環(huán)節(jié)到校外單位完成,一種全新的培養(yǎng)模式。本論文結(jié)合臨床中藥學(xué)專業(yè)學(xué)位研究生培養(yǎng)要求,分為實驗研究和藥學(xué)實踐兩部分。第一部分,實驗研究部分。首先是文獻綜述,該部分闡述了特發(fā)性肺間質(zhì)纖維化的病因病機、中醫(yī)藥治療現(xiàn)狀,以及特發(fā)性肺間質(zhì)纖維化目前治療過程中存在在的問題并提出合理的建議;綜述了肺痿顆粒中主要藥味的化學(xué)成分和藥理作用研究進展。其次是肺痿顆粒的制備工藝研究,結(jié)合工藝路線建立肺痿顆粒中各藥味以及水提和醇提的HPLC指紋圖譜,并將其運用到工藝優(yōu)化環(huán)節(jié),與傳統(tǒng)以單指標為指標的工藝優(yōu)化研究方法進行對比分析,優(yōu)選出最佳提取工藝,干燥工藝,結(jié)合正交設(shè)計優(yōu)選肺痿顆粒的成型制備工藝。實驗研究內(nèi)容及結(jié)果如下:目的和意義特發(fā)性肺間質(zhì)纖維化是一種病因不明,發(fā)病機制不清,缺乏治療手段,以彌漫性肺泡炎和肺泡結(jié)構(gòu)紊亂的慢性纖維化性間質(zhì)性肺疾病。平均生存期僅2年,5年生存率不足50%。目前西醫(yī)學(xué)對尚缺乏特異性的治療手段,尋找新的更為安全有效的治療藥物具有重要的現(xiàn)實意義。為了方便患者臨床應(yīng)用,將其制成顆粒劑。肺痿顆粒由人參、三七、紫菀、五味子、山萸肉等藥味中藥組成,具有補益肺腎,益氣活血之功效。本文在中醫(yī)藥理論指導(dǎo)下,結(jié)合處方中各藥的理化性質(zhì)及藥理活性,從處方飲片的指紋圖譜、提取工藝及成型工藝方面進行系統(tǒng)性研究,從而為臨床治療提供安全、有效、服用方便的肺痿顆粒。方法1建立肺痿顆粒主要藥材飲片的HPLC指紋圖譜:根據(jù)藥材理化性質(zhì)和藥理活性確定處方的工藝路線,再依據(jù)工藝路線,分別建立飲片、醇提液和水提液的HPLC指紋圖譜,對比飲片和醇提液、水提液的HPLC指紋圖譜,指出各味藥的專屬峰和共有峰。2提取工藝研究:采用正交試驗設(shè)計,以峰面積,指標成分,得稿率為考察指標,進行多指標綜合評價優(yōu)選出最佳提取工藝,并與單指標定量方法優(yōu)選出的最佳工藝作對比,確定肺痿顆粒的最佳醇提工藝、水提醇沉工藝和干燥工藝,并對該優(yōu)化工藝進行驗證。3成型工藝研究:設(shè)計正交試驗,以顆粒成型率、吸濕率、溶化性作為考察指標進行指標綜合評價,篩選最佳制劑處方;采用擠出制粒法制粒。對制得的顆粒進行水分、粒度、吸濕性等項目考察。結(jié)果1人參和三七醇提,山萸肉、五味子、紫菀、麥冬、白果等味藥水提醇沉,從醇提液HPLC的指紋圖譜中選取了 24個峰,水提液的HPLC的指紋圖譜中指認出13個峰。2最佳醇提工藝條件為10倍量60%乙醇,提取3次,每次1h;水提醇沉的最佳工藝條件為8倍量水,提取2次,每次2小時;水提液濃縮至密度至1.11～1.15(室溫測),加入95%的乙醇使含醇量達到60%,放置18h。將醇提液和水提液合并濃縮至稠膏,在80℃條件下減壓干燥至干浸膏。證明了多指標成分定量法較單指標定量法更加準確和全面。3最佳成型制備工藝為:將干浸膏粉碎,加入1:0.5的輔料,混合均勻,在攪拌下加入8%的干浸膏量的90%乙醇(噴淋的方式加入),制軟才,將制得的軟材用14目篩制粒,80℃下干燥3小時,14目篩整粒。水分、粒度、流動性均符合藥典規(guī)定。結(jié)論本研究確定的肺痿顆粒提取工藝、干燥工藝、制劑成型工藝合理、穩(wěn)定、簡單可行。實踐研究內(nèi)容及結(jié)果如下:本文通過藥學(xué)實踐部分旨在通過對中日友好醫(yī)院臨床中藥學(xué)服務(wù)現(xiàn)狀研究,改善臨床中藥學(xué)服務(wù)模式,提高服務(wù)質(zhì)量,保障患者的合埋用藥。作者通過在衛(wèi)計委中日友好醫(yī)院2年多的實踐,對該院已開展的中藥臨床藥學(xué)服務(wù)項目內(nèi)容、服務(wù)方法、服務(wù)水平和服務(wù)質(zhì)量等相關(guān)資料進行綜合概括、分析,研究該院中藥臨床學(xué)服務(wù)工作開展情況。對比分析臨床藥學(xué)和中藥臨床藥學(xué)服務(wù)模式,并為中藥臨床藥學(xué)服務(wù)提出以下幾點建議:1完善相關(guān)法律法規(guī)及行業(yè)規(guī)范體系;2加強對藥學(xué)服務(wù)的政策支持;3改變藥房調(diào)劑模式,發(fā)揮藥師專業(yè)作用;4加強藥師人才培養(yǎng);5提高藥學(xué)科研水平。
[Abstract]:Professional degree is an important way to train special professionals of high level and innovative application type. In recent years, it has become an important form of graduate education in China. As a unique medical system and the extension of Chinese culture, traditional Chinese medicine has been highly valued by the state. As a result, the degree committee of the State Council decides to set up a master's degree in Chinese pharmacy and is the first to recruit a graduate student of professional degree in Chinese pharmacy in 2011. The aim is to cultivate high level compound talents with practical ability and innovative application ability in order to adapt to the modernization of Chinese medicine industry and the diversified development model. Master degree is a kind of advanced talent training model which is different from academic master. Professional degree adopts "school enterprise cooperation", "dual tutor system" mode of "school enterprise, school, business, enterprise". The theory course is based on school, practice link to out of school units, a new training mode. This paper combines with clinical pharmacy major. The training requirements of graduate students are divided into two parts: experimental research and pharmaceutical practice. The first part, the experimental research part. First, the literature review. This part expounds the etiology and pathogenesis of idiopathic pulmonary fibrosis, the status of traditional Chinese medicine treatment, and the problems in the treatment process of idiopathic pulmonary fibrosis. The research progress on the chemical composition and pharmacological action of the main medicinal herbs in the lung flaccid granules was summarized. Secondly, the preparation technology of the lung flaccid granules was studied. The HPLC fingerprint of the drugs in the lung granule and the water extraction and alcohol extraction were established by combining the process route. The optimum extraction process, drying process, and orthogonal design were selected to optimize the preparation process of lung flaccid granules. The contents and results were as follows: objective and significance idiopathic pulmonary fibrosis is a kind of unknown etiology, unclear pathogenesis, lack of treatment, diffuse alveolitis and alveoli. Chronic fibrotic interstitial lung disease with structural disorder. The average survival time is only 2 years, and the 5 year survival rate is less than 50%.. At present, western medicine is of great practical significance for the lack of specific treatment and finding new and more safe and effective treatment drugs. 37, the medicinal herbs of aster, Schisandra chinensis and Cornus officinalis have the effect of tonifying lung and kidney, promoting qi and promoting blood circulation. This article, under the guidance of traditional Chinese medicine theory, combines the physical and chemical properties and pharmacological activities of each prescription in the prescription, and systematically studies the fingerprint of prescription pieces, the extraction technology and the molding technology, thus providing a safety for clinical treatment. All, effective, take the convenient lung flaccid granules. Method 1 the HPLC fingerprint of the main medicinal herbs of Fei Wei granule was established. According to the physicochemical and pharmacological activity of the medicinal materials, the HPLC fingerprints of the decoction pieces, alcohol extract and water extract were established according to the technological route, and the HPLC fingerprints of the water extract and the water extract were compared. The special peak of each flavour and the extraction process of the common peak.2 were studied. The optimum extraction process was selected by orthogonal experiment design with the peak area, the index component and the draft rate as the inspection index, and the optimum extraction technology was selected by multi index comprehensive evaluation, and the optimum extraction technology of the lung flaccid granules was determined by comparison with the best process selected by the single index quantitative method. Water extraction and alcohol precipitation process and drying technology, and the optimization of the process to verify the.3 molding process: design orthogonal test, taking the particle molding rate, hygroscopic rate and solubility as an index to evaluate the index synthetically, screening the best preparation prescription, using the extrusion granulating particle. Results 1 ginseng and 37 alcohol extraction, the flavour of Cornus, Schisandra, aster, Ophiopogon, and white fruit were extracted and alcohols, and 24 peaks were selected from the HPLC fingerprint of the alcohol extract. The fingerprint of the HPLC of the water extract was identified as the optimum alcohol extraction process of 13 peaks, 10 times 60% ethanol, 3 times, each 1H, and the best process bar for water extraction and alcohol precipitation. 8 times the amount of water, 2 times, 2 hours each time; the water extract is concentrated to 1.11 ~ 1.15 (Shi Wen test) and 95% ethanol is added to the alcohol content to 60%. The alcohol extract and water extract are concentrated to the thickening paste by 18h., and then dried to dry extract at 80 C. It is proved that the quantitative method is more accurate than the single index quantitative method. The optimum preparation process of the comprehensive.3 molding is to crush the dry extract, add the auxiliary material of 1:0.5, mix evenly, add 8% dry extract of 90% ethanol (spray method) under stirring, make soft material, make the soft wood with 14 mesh screens, dry 3 hours at 80 degrees C, and screen the whole grain. Water, grain size and fluidity all conform to the stipulations of the Pharmacopoeia. To study the extraction process of lung flaccid particles, the drying process, the preparation process is reasonable, stable, simple and feasible. The contents and results of the practice are as follows: This article aims to improve the service mode of clinical traditional Chinese medicine through the research on the clinical pharmacy service in China-Japan Friendship Hospital, improve the quality of service and guarantee the patient Through more than 2 years' practice in China-Japan Friendship Hospital of Wei Planning Commission, the author comprehensively summarized and analyzed the contents, service methods, service level and service quality of TCM clinical pharmacy service projects that had been carried out in the hospital, analyzed the development of clinical service work of Chinese medicine in the hospital, compared and analyzed clinical pharmacy and Chinese medicine. Bed pharmacy service model, and to provide the following suggestions for the clinical pharmacy service of Chinese medicine: 1 improve relevant laws and regulations and industry standard system; 2 strengthen the policy support for pharmaceutical services; 3 change the mode of pharmacy dispensing, play the professional role of pharmacists; 4 strengthen the training of pharmacists; 5 improve the level of pharmaceutical research.

【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R283.6;R288

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本文編號：1844673