【摘要】：目的建立1種相對簡便的測量紅細胞抗體親和力方法,以之研究親和力因素對紅細胞抗體檢測的影響。方法根據(jù)親和力公式,在紅細胞與抗體反應達到平衡時,通過測量游離抗體濃度以及計算紅細胞表面結(jié)合與未結(jié)合抗體的抗原比例,可計算出抗體與相應紅細胞的結(jié)合常數(shù)(Ka)即親和力。應用定量的R1R1紅細胞吸收高效價人源抗-D,先測定飽和吸收抗-D的量,再測量不同濃度抗-D被定量紅細胞吸收后游離抗體濃度,計算紅細胞結(jié)合與未結(jié)合抗-D的D抗原的比值,推導出人源抗-D與R1R1細胞反應的結(jié)合常數(shù)。結(jié)果檢測中使用的人源抗-D與R1R1細胞反應親和力為(2.1-7.6)×108;實驗顯示,紅細胞結(jié)合抗體量越少,親和力相對越高。平行檢測的1例單克隆Ig G抗-D的親和力約為4.6×109,明顯高于人源抗-D。結(jié)論所建立的測量紅細胞抗體親和力方法更為簡便,減少了出現(xiàn)偏差的機會,適用于基礎、臨床以及檢測的方法研究。
[Abstract]:Objective to establish a relatively simple method for the determination of erythrocyte antibody affinity and to study the effect of affinity factors on erythrocyte antibody detection. Methods according to the formula of affinity, when the reaction between erythrocyte and antibody reached equilibrium, the concentration of free antibody and the antigen ratio of surface binding and unbinding antibody of erythrocyte were calculated by measuring the concentration of free antibody. The binding constant (Ka) of the antibody and the corresponding red blood cell can be calculated. Using the quantitative R1R1 red blood cell absorption high titer human anti-D, the saturated absorption anti-D was measured first, then the free antibody concentration after the different concentration of anti-D was absorbed by the quantitative red blood cell was measured. The ratio of red blood cell binding to unbound D antigen was calculated and the binding constant of human anti D to R1R1 cells was derived. Results the affinity of human anti-D to R1R1 cells was (2.1-7.6) 脳 10 ~ (8), which showed that the lower the amount of erythrocyte binding antibody was, the higher the affinity was. The affinity of monoclonal Ig G anti-D was 4.6 脳 109, which was significantly higher than that of human anti-D. Conclusion the established method for measuring the affinity of red blood cell antibodies is more convenient and less prone to deviation. It is suitable for basic, clinical and detection methods.
【作者單位】： 上海市血液中心;
【基金】：上海市公共衛(wèi)生重點學科建設項目(15GWZK0501)
【分類號】：R457.1

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