【摘要】：目的建立1種相對(duì)簡(jiǎn)便的測(cè)量紅細(xì)胞抗體親和力方法,以之研究親和力因素對(duì)紅細(xì)胞抗體檢測(cè)的影響。方法根據(jù)親和力公式,在紅細(xì)胞與抗體反應(yīng)達(dá)到平衡時(shí),通過(guò)測(cè)量游離抗體濃度以及計(jì)算紅細(xì)胞表面結(jié)合與未結(jié)合抗體的抗原比例,可計(jì)算出抗體與相應(yīng)紅細(xì)胞的結(jié)合常數(shù)(Ka)即親和力。應(yīng)用定量的R1R1紅細(xì)胞吸收高效價(jià)人源抗-D,先測(cè)定飽和吸收抗-D的量,再測(cè)量不同濃度抗-D被定量紅細(xì)胞吸收后游離抗體濃度,計(jì)算紅細(xì)胞結(jié)合與未結(jié)合抗-D的D抗原的比值,推導(dǎo)出人源抗-D與R1R1細(xì)胞反應(yīng)的結(jié)合常數(shù)。結(jié)果檢測(cè)中使用的人源抗-D與R1R1細(xì)胞反應(yīng)親和力為(2.1-7.6)×108;實(shí)驗(yàn)顯示,紅細(xì)胞結(jié)合抗體量越少,親和力相對(duì)越高。平行檢測(cè)的1例單克隆Ig G抗-D的親和力約為4.6×109,明顯高于人源抗-D。結(jié)論所建立的測(cè)量紅細(xì)胞抗體親和力方法更為簡(jiǎn)便,減少了出現(xiàn)偏差的機(jī)會(huì),適用于基礎(chǔ)、臨床以及檢測(cè)的方法研究。
[Abstract]:Objective to establish a relatively simple method for the determination of erythrocyte antibody affinity and to study the effect of affinity factors on erythrocyte antibody detection. Methods according to the formula of affinity, when the reaction between erythrocyte and antibody reached equilibrium, the concentration of free antibody and the antigen ratio of surface binding and unbinding antibody of erythrocyte were calculated by measuring the concentration of free antibody. The binding constant (Ka) of the antibody and the corresponding red blood cell can be calculated. Using the quantitative R1R1 red blood cell absorption high titer human anti-D, the saturated absorption anti-D was measured first, then the free antibody concentration after the different concentration of anti-D was absorbed by the quantitative red blood cell was measured. The ratio of red blood cell binding to unbound D antigen was calculated and the binding constant of human anti D to R1R1 cells was derived. Results the affinity of human anti-D to R1R1 cells was (2.1-7.6) 脳 10 ~ (8), which showed that the lower the amount of erythrocyte binding antibody was, the higher the affinity was. The affinity of monoclonal Ig G anti-D was 4.6 脳 109, which was significantly higher than that of human anti-D. Conclusion the established method for measuring the affinity of red blood cell antibodies is more convenient and less prone to deviation. It is suitable for basic, clinical and detection methods.
【作者單位】： 上海市血液中心;
【基金】：上海市公共衛(wèi)生重點(diǎn)學(xué)科建設(shè)項(xiàng)目(15GWZK0501)
【分類號(hào)】：R457.1

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