發(fā)布時(shí)間：2018-11-05 06:59

【摘要】：目的建立使用流式細(xì)胞儀檢測(cè)血小板跨膜電位的方法,觀察血小板在體外保存期間跨膜電位變化。方法使用電壓敏感染料Di BAC4(3)對(duì)20(人)份血小板(20 m L/份)染色后,用流式細(xì)胞儀測(cè)定血小板在靜息狀態(tài)下和完全去極化時(shí)的熒光強(qiáng)度,根據(jù)能斯特方程計(jì)算血小板跨膜電位,并利用此方法觀察血小板在體外保存期間跨膜電位的變化情況。結(jié)果 20人份新鮮血小板跨膜電位平均值為(-56±4)m V,CV=5.5%,與膜電位鉗法得到的結(jié)果(-50-60)m V相似,保存1、3、5 d的血小板跨膜電位分別為(-56±4)、(-54±8)和(-46±6)m V,其中血小板跨膜電位在血小板保存1 d時(shí)明顯高于5 d(P0.05)。結(jié)論所建立起的方法用于測(cè)定血小板跨膜電位準(zhǔn)確、可靠。
[Abstract]:Objective to establish a flow cytometry method to detect the transmembrane potential of platelets and observe the changes of the transmembrane potential of platelets during in vitro preservation. Methods Di BAC4 (3), a voltage-sensitive dye, was used to stain 20 (human) platelets (20ml / phr). The fluorescence intensity of platelets was measured by flow cytometry at rest and at complete depolarization. The transmembrane potential of platelets was calculated according to Nernst equation, and the change of transmembrane potential of platelets during in vitro preservation was observed by this method. Results the mean transmembrane potential of 20 fresh platelets was (-56 鹵4) MV CVV 5.5, which was similar to that obtained by membrane potential clamp method (-50-60) m V), and the transmembrane potential of platelets preserved for 3 days was (-56 鹵4), similar to that obtained by membrane potential clamp method (-50-60) m V). In (-54 鹵8) and (-46 鹵6) m V, the transmembrane potential of platelet was significantly higher than that of 5 days after platelet preservation for 1 day (P0.05). Conclusion the established method is accurate and reliable for the determination of platelet transmembrane potential.
【作者單位】： 華東師范大學(xué)生命科學(xué)學(xué)院;上海市血液中心;
【基金】：上海市衛(wèi)生與計(jì)劃生育委員會(huì)科研項(xiàng)目(20124240) 上海市公益衛(wèi)生行業(yè)專項(xiàng)(201002005)
【分類號(hào)】：R446.11
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本文編號(hào)：2311277