【摘要】：目的探討全血標(biāo)本離心前不同溫度與時(shí)間對(duì)人巨細(xì)胞病毒(human cytomegalovirus,HCMV)核酸檢測(cè)(nucleic acid amplification test,NAT)結(jié)果的影響,以確定離心前放置的安全時(shí)限。方法采集含高、中、低DNA載量的全血。采血后按保存溫度分為4℃、25℃、37℃組,各組放置0、1、6、12、24、48、72 h后進(jìn)行離心,行實(shí)時(shí)熒光定量PCR。結(jié)果高濃度4℃組離心前放置72 h,25℃與37℃組離心前放置24 h,無明顯變化;中濃度4℃組離心前放置24 h,25℃組、37℃組離心前放置12 h,無明顯變化;低濃度4℃組、25℃組離心前放置12 h,37℃組離心前放置6 h,無明顯變化。結(jié)論為保證HCMV NAT結(jié)果的準(zhǔn)確性,全血標(biāo)本最好在離心前4℃保存,并在采血后6 h內(nèi)及時(shí)離心送檢,高溫條件下更應(yīng)注意。
[Abstract]:Objective to investigate the effect of different temperature and time before centrifugation on the detection of human cytomegalovirus (human cytomegalovirus,HCMV) nucleic acid (nucleic acid amplification test,NAT) in whole blood samples, and to determine the safe time limit for the detection of human cytomegalovirus (human cytomegalovirus,HCMV) nucleic acid before centrifugation. Methods whole blood containing high, middle and low DNA load was collected. The blood samples were divided into 4 鈩，

本文編號(hào)：2248442