【摘要】：目的副流感病毒是嬰幼兒下呼吸道感染的重要病原體,為快速準(zhǔn)確診斷副流感病毒感染,文中探討一種從鼻拭子快速分離培養(yǎng)及鑒定副流感病毒的方法。方法采集0~5歲診斷為急性呼吸道感染的患兒鼻拭子標(biāo)本,以3000r/min離心1 h接種至96孔板預(yù)制LLC-MK2細(xì)胞以及按照傳統(tǒng)培養(yǎng)法進行接種,最后補充含4μg/m L TPCK胰酶的病毒維持培養(yǎng)基。每天觀察細(xì)胞病變,培養(yǎng)第2、5、8天分別作血吸附、血凝試驗,試驗陽性時作免疫熒光鑒定。結(jié)果從83份鼻拭子標(biāo)本中分離鑒定6株副流感病毒,陽性率7.2%。離心培養(yǎng)法與傳統(tǒng)培養(yǎng)法在標(biāo)本培養(yǎng)2 d后其分離率分別為7.2%和0,差異有統(tǒng)計學(xué)意義(P0.05)。陽性標(biāo)本感染的細(xì)胞鏡下可見局部變長、融合、破碎的細(xì)胞病變,血凝試驗4℃凝集而室溫不凝集,血吸附試驗4℃陽性而室溫陰性,免疫熒光可見細(xì)胞內(nèi)特異性蘋果綠熒光。結(jié)論快速分離培養(yǎng)方法可最快在2 d內(nèi)從鼻拭子標(biāo)本中獲得有毒力和生物活性的副流感病毒并進行鑒定。
[Abstract]:Objective Parainfluenza virus is an important pathogen of infantile lower respiratory tract infection. In order to diagnose parainfluenza virus infection quickly and accurately, a method of rapid isolation, culture and identification of parainfluenza virus from nasal swab is discussed. Methods nasal swabs were collected from children aged 0 to 5 years with acute respiratory tract infection. Prefabricated LLC-MK2 cells were inoculated with 3000r/min centrifugation for 1 h to 96 well plates and inoculated according to the traditional culture method. Finally, the virus maintenance medium containing 4 渭 g / mL TPCK trypsin was added. The cytopathic changes were observed every day. Blood adsorption, hemagglutination test and immunofluorescence assay were performed on the 5th day of culture. Results 6 strains of parainfluenza virus were isolated from 83 nasal swabs and the positive rate was 7.2%. The isolation rates of centrifuge culture method and traditional culture method were 7.2% and 0 respectively after 2 days of culture. The difference was statistically significant (P0.05). The infected cells of positive specimens showed local lengthening, fusion, broken cytopathic changes, hemagglutination test 4 鈩，

本文編號：2168998