本文選題：乳腺腫瘤　切入點(diǎn)：淋巴轉(zhuǎn)移　出處：《中國醫(yī)學(xué)影像學(xué)雜志》2016年06期 　論文類型：期刊論文

【摘要】：目的現(xiàn)行方法建立的乳腺癌動物模型多用于觀察其生物學(xué)表現(xiàn),不適于探索新的淋巴結(jié)轉(zhuǎn)移治療方案。本研究通過改良制作方法建立新型的乳腺癌淋巴結(jié)轉(zhuǎn)移模型,為探索新的治療方法提供實驗動物模型。材料與方法采用組織塊包埋法將VX2傳代瘤株種植于30只雌性兔左側(cè)第3只乳頭的乳墊內(nèi),每周采用觸診法和常規(guī)超聲查看腫瘤及淋巴結(jié)生長情況。待觸及腫大的淋巴結(jié)后切除乳腺癌組織。第4周隨機(jī)摘取15枚淋巴結(jié),采用病理學(xué)觀察淋巴結(jié)形態(tài)及組織學(xué)改變。將游標(biāo)卡尺與超聲測量結(jié)果進(jìn)行對照,比較其差異。結(jié)果 2周后,26只實驗兔可觸及乳腺內(nèi)質(zhì)韌的類圓形結(jié)節(jié);超聲呈低回聲,邊界欠清,內(nèi)部及周邊血流豐富。約3周后,23只實驗兔的左側(cè)腋窩處可觸及腫大的淋巴結(jié),病理證實為轉(zhuǎn)移性淋巴結(jié),淋巴結(jié)轉(zhuǎn)移率為88.5%,造模成功率為76.7%。超聲與病理兩種方法測量淋巴結(jié)最大直徑差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論改良法建立乳腺癌淋巴結(jié)轉(zhuǎn)移模型周期短、成功率高、方法簡單,能夠為研究新療法提供動物模型;常規(guī)超聲能夠?qū)崟r監(jiān)測腫瘤的生長狀況,操作方便、快捷。
[Abstract]:Objective the animal models of breast cancer established by the present method are mostly used to observe their biological manifestations and are not suitable to explore a new treatment scheme for lymph node metastasis. In this study, a new model of lymph node metastasis of breast cancer was established by modified method. Materials and methods VX2 subculture was implanted into the breast pad of the third third nipple on the left side of 30 female rabbits by tissue mass embedding. The tumor and lymph node growth were examined by palpation and conventional ultrasound every week. 15 lymph nodes were randomly removed at 4 weeks after resection of breast cancer tissues after touching enlarged lymph nodes. The morphological and histological changes of lymph nodes were observed by pathology. The results of Vernier caliper were compared with the results of ultrasound. Results after 2 weeks, 26 rabbits could reach the ductile round nodules of mammary gland. The left axillary lymph nodes of 23 experimental rabbits were palpable at the left armpit about 3 weeks later. The lymph nodes were confirmed as metastatic lymph nodes by pathology. The lymph node metastasis rate was 88.5 and the success rate was 76.70.There was no significant difference between ultrasound and pathology in the measurement of lymph node maximum diameter. Conclusion the modified method has the advantages of short period, high success rate and simple method for establishing lymph node metastasis model of breast cancer. It can provide animal model for the study of new therapy, and conventional ultrasound can monitor tumor growth in real time, which is convenient and fast.
【作者單位】： 濰坊醫(yī)學(xué)院醫(yī)學(xué)影像學(xué)系;濰坊醫(yī)學(xué)院心理學(xué)系;
【分類號】：R445.1;R737.9

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