本文選題：獻(xiàn)血者　切入點(diǎn)：抗-HCV　出處：《中國輸血雜志》2016年06期 　論文類型：期刊論文

【摘要】：目的評估獻(xiàn)血人群抗-HCV ELISA試劑的初篩效果、重組免疫印跡試驗(yàn)(RIBA補(bǔ)充實(shí)驗(yàn))和HCV RNA核酸檢測的情況,以探索無償獻(xiàn)血人群抗-HCV的篩查效果。方法收集2013年5月31日-2015年1月20日118 350例標(biāo)本,采用2個(gè)不同廠家抗-HCV ELISA初篩試劑檢測,初篩有反應(yīng)性的標(biāo)本(S/CO≥0.75)采用RIBA的方法進(jìn)行補(bǔ)充實(shí)驗(yàn),RIBA補(bǔ)充實(shí)驗(yàn)不確定結(jié)果的標(biāo)本進(jìn)行核酸檢測。結(jié)果 2種初篩酶免試劑檢測結(jié)果的陽性符合率為64.6%,陰性符合率為99.9%,總符合率為99.9%。北京萬泰抗-HCV抗體診斷試劑盒檢測陽性標(biāo)本93.3%分布于S/CO≥10.0以上,上�？迫A抗-HCV抗體診斷試劑盒檢測陽性標(biāo)本91.3%分布于S/CO≥7.0以上。2種初篩試劑單邊陽性的結(jié)果中,RIBA補(bǔ)充實(shí)驗(yàn)總陽性結(jié)果的比例為2.7%,不確定結(jié)果的比例為18.4%,陰性結(jié)果的比例為78.9%,北京萬泰試劑單邊陽性對應(yīng)有2例RIBA陽性結(jié)果,上�？迫A試劑單邊陽性對應(yīng)有1例RIBA陽性結(jié)果,2種試劑檢測性能具有一定的互補(bǔ)性。初篩單邊陽性及RIBA補(bǔ)充實(shí)驗(yàn)結(jié)果為不確定的標(biāo)本核酸檢測均為陰性。RIBA陽性標(biāo)本條帶分析結(jié)果中,Core、NS3、NS4.1、NS4.2、NS5條帶所占的比例分別為35.4%、33.9%、14.9%、2.4%、13.4%;RIBA不確定結(jié)果中,僅有Core、NS3 2種條帶,比例為49.2%、50.8%。結(jié)論在獻(xiàn)血人群中抗-HCV ELISA試劑檢測存在生物學(xué)上的假陽性,一定程度上造成血液資源的浪費(fèi),選擇靈敏度高、特異性好的初篩試劑,輔以RIBA補(bǔ)充實(shí)驗(yàn),對于保證輸血安全具有非常重要的意義。
[Abstract]:Objective to evaluate the screening effect of anti-HCV ELISA reagent, recombinant Western blotting test (RIBA-supplementation test) and detection of HCV RNA nucleic acid in blood donors. Methods 118,350 samples from May 31st 2013 to January 20th 2015 were collected and tested by two different anti-HCV ELISA screening reagents. RIBA method was used to detect nucleic acid in samples with uncertain results of RIBA supplementation experiment. Results the positive coincidence rate of the results of the two enzyme immunoassay was 64.6 and the negative rate was 64.6. The coincident rate was 99.9 and the total coincidence rate was 99.9.The positive specimens of Beijing Wantai anti-HCV antibody diagnostic kit were found to be above 10. 0 or more in S- / CO ratio. 91.3% positive samples of Shanghai Kehua Anti-HCV Antibody Diagnostic Kit were found in the results of S- / CO 鈮，

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