高蛋白類原料的產(chǎn)甲烷性能和微生物群落動(dòng)態(tài)
發(fā)布時(shí)間:2021-03-30 04:40
由于日益激增的能源危機(jī)和環(huán)境問題,人們將越來越多的注意力聚焦于一種新的生物燃料——生物質(zhì)資源。厭氧消化(AD)技術(shù)應(yīng)用廣泛,可將生物質(zhì)資源(如有機(jī)廢棄物)轉(zhuǎn)化為生物甲烷,生產(chǎn)可再生能源。不同生化性質(zhì)的食品廢棄物是有機(jī)廢棄物的重要組成部分之一。在中國,快速增長的經(jīng)濟(jì)推動(dòng)了飲食結(jié)構(gòu)的急劇改變,其中,富含蛋白質(zhì)的食物被大量使用,包括海產(chǎn)品、肉類(豬肉、牛肉和羊肉)、乳制品、家禽、谷物和豆類等。因此,每年會(huì)產(chǎn)生大量高蛋白含量的食品廢棄物,造成嚴(yán)重的環(huán)境污染。這些高蛋白類廢棄物可以有效地被厭氧消化利用,進(jìn)行甲烷生產(chǎn)。在厭氧消化過程中,蛋白質(zhì)首先被水解成20種氨基酸,然后被發(fā)酵成短鏈或支鏈有機(jī)酸,最后轉(zhuǎn)化為終產(chǎn)物甲烷。然而,每種氨基酸都有不同的產(chǎn)甲烷性能和生物降解性,所以高蛋白類食品廢棄物的消化特性在很大程度上取決于其氨基酸的組成和濃度。因此,為了在厭氧消化中有效利用高蛋白類廢棄物,有必要對每種氨基酸的產(chǎn)甲烷性能進(jìn)行研究。本課題系統(tǒng)地研究了 20種不同氨基酸的厭氧消化性能和微生物群落變化。隨后,分別在中溫和高溫條件下,研究了蛋白質(zhì)含量高或適中的食物樣品的厭氧消化性能和微生物群落結(jié)構(gòu)。最后,研究了生...
【文章來源】:北京化工大學(xué)北京市 211工程院校 教育部直屬院校
【文章頁數(shù)】:124 頁
【學(xué)位級別】:博士
【文章目錄】:
學(xué)位論文數(shù)據(jù)集
Abstract
摘要
List of abbreviations
Chapter 1 Introduction
1.1 Global fossil fuel consumption and transition to renewable energy
1.2 Protein waste generation
1.3 Mechanism of anaerobic digestion
1.4 Prospects of protein utilization for methane production via AD
1.5 Strategies to enhance methane production performance
1.5.1 Temperature based enhancement
1.5.2 Use of biochar as additives
1.6 Feasibility of AD projects utilizing municipal solid waste in China according to 13h five-year plan
1.7 Research objectives,content, and route
1.7.1 Research objectives
1.7.2 Research content and route
Chapter 2 Experimental materials and methods
2.1 Substrates, biochar, and inoculum
2.2 Laboratory reagents and equipment
2.3 Anaerobic digester
2.4 Analytical methods
2.5 Biochemical composition analysis of substrates
2.5.1 Determination of total carbohydrates
2.5.2 Determination of crude lipid
2.5.3 Determination of crude Protein
2.5.4 Determination of amino acids
2.6 Maximal theoretical methane yield and biodegradability
2.7 Calculation of methane production
2.8 Kinetic models for the methane production during anaerobic digestion
2.9 16S rRNA gene sequencing and analysis
2.10 Indexes indicating biological community diversity and richness
2.10.1 Shannon index
2.10.2 Chaol index
2.10.3 ACE index
2.10.4 Simpson index
2.10.5 Coverage
2.11 Data analysis and processing
Chapter 3 Biomethane production of protein-constituent amino acids and associatedmicrobial community changes during anaerobic digestion
3.1 Materials
3.2 Experimental design
3.3 Results and discussions
3.3.1 Daily methane yield
3.3.2 Cumulative methane yield
3.3.3 Biodegradability
3.3.4 Kinetic analysis of a modified Gompertz model
3.3.5 Anaerobic digestion stability
3.3.6 Microbial community analysis
3.3.6.1 Species diversity and richness
3.3.6.2 Bacterial genera
3.3.6.3 Archaeal genera
3.4 Summary
Chapter 4 Comparing mesophilic and thermophilic anaerobic digestion of protein substrates:methane production performance, amino acid profile, and predominant microbialcommunities
4.1 Materials
4.2 Experiment design
4.3. Results and discussions
4.3.1 Characteristics of substrates
4.3.2 Daily methane yield
4.3.3 Cumulative methane yield
4.3.4 Biodegradability
4.3.5 Kinetic analysis of a modified Gompertz model
4.3.6 Anaerobic digestion stability
4.3.7 Correlation analysis
4.3.8 Microbial community analysis
4.3.8.1 Species diversity and richness
4.3.8.2 Bacterial genera
4.3.8.3 Archaeal genera
4.4 Summary
Chapter 5 Impact of biochar addition on methane production performance of differentprotein substrates
5.1 Materials
5.2 Experimental design
5.3 Results and discussions
5.3.1 Characteristics of substrates and biochar
5.3.2 Daily methane production
5.3.3 Cumulative methane production
5.3.4 Biomethane production potential and biodegradability
5.3.5 Kinetic model evaluation
5.3.6 Anaerobic digestion stability
5.4 Summary
th five-year plan">Chapter 6 Economic factors and cost benefit analysis of AD plants utilizing MSW in Chinaaccording to 13th five-year plan
6.1 Economic factors
6.1.1 Markets
6.1.2 Capitals
6.1.3 Financing modes
6.2 Cost benefit analysis
6.2.1 Investment costs
6.2.2 Operational costs
6.3 Case of a typical BOT funded AD project in China
6.4 Summary
Chapter 7 Conclusions
7.1 Main conclusions
7.2 Innovation
7.3 Research prospects
References
Acknowledgements
List of publications
Introduction of author and supervisor
附件
【參考文獻(xiàn)】:
期刊論文
[1]Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy[J]. Yuan Yue,Zi-Dong Qiu,Xian-You Qu,Ai-Ping Deng,Yuan Yuan,Lu-Qi Huang,Chang-Jiang-Sheng Lai. Journal of Pharmaceutical Analysis. 2018(05)
[2]Effects of free ammonia on volatile fatty acid accumulation and process performance in the anaerobic digestion of two typical bio-wastes[J]. Xuchuan Shi,Jia Lin,Jiane Zuo,Peng Li,Xiaoxia Li,Xianglin Guo. Journal of Environmental Sciences. 2017(05)
本文編號:3108879
【文章來源】:北京化工大學(xué)北京市 211工程院校 教育部直屬院校
【文章頁數(shù)】:124 頁
【學(xué)位級別】:博士
【文章目錄】:
學(xué)位論文數(shù)據(jù)集
Abstract
摘要
List of abbreviations
Chapter 1 Introduction
1.1 Global fossil fuel consumption and transition to renewable energy
1.2 Protein waste generation
1.3 Mechanism of anaerobic digestion
1.4 Prospects of protein utilization for methane production via AD
1.5 Strategies to enhance methane production performance
1.5.1 Temperature based enhancement
1.5.2 Use of biochar as additives
1.6 Feasibility of AD projects utilizing municipal solid waste in China according to 13h five-year plan
1.7 Research objectives,content, and route
1.7.1 Research objectives
1.7.2 Research content and route
Chapter 2 Experimental materials and methods
2.1 Substrates, biochar, and inoculum
2.2 Laboratory reagents and equipment
2.3 Anaerobic digester
2.4 Analytical methods
2.5 Biochemical composition analysis of substrates
2.5.1 Determination of total carbohydrates
2.5.2 Determination of crude lipid
2.5.3 Determination of crude Protein
2.5.4 Determination of amino acids
2.6 Maximal theoretical methane yield and biodegradability
2.7 Calculation of methane production
2.8 Kinetic models for the methane production during anaerobic digestion
2.9 16S rRNA gene sequencing and analysis
2.10 Indexes indicating biological community diversity and richness
2.10.1 Shannon index
2.10.2 Chaol index
2.10.3 ACE index
2.10.4 Simpson index
2.10.5 Coverage
2.11 Data analysis and processing
Chapter 3 Biomethane production of protein-constituent amino acids and associatedmicrobial community changes during anaerobic digestion
3.1 Materials
3.2 Experimental design
3.3 Results and discussions
3.3.1 Daily methane yield
3.3.2 Cumulative methane yield
3.3.3 Biodegradability
3.3.4 Kinetic analysis of a modified Gompertz model
3.3.5 Anaerobic digestion stability
3.3.6 Microbial community analysis
3.3.6.1 Species diversity and richness
3.3.6.2 Bacterial genera
3.3.6.3 Archaeal genera
3.4 Summary
Chapter 4 Comparing mesophilic and thermophilic anaerobic digestion of protein substrates:methane production performance, amino acid profile, and predominant microbialcommunities
4.1 Materials
4.2 Experiment design
4.3. Results and discussions
4.3.1 Characteristics of substrates
4.3.2 Daily methane yield
4.3.3 Cumulative methane yield
4.3.4 Biodegradability
4.3.5 Kinetic analysis of a modified Gompertz model
4.3.6 Anaerobic digestion stability
4.3.7 Correlation analysis
4.3.8 Microbial community analysis
4.3.8.1 Species diversity and richness
4.3.8.2 Bacterial genera
4.3.8.3 Archaeal genera
4.4 Summary
Chapter 5 Impact of biochar addition on methane production performance of differentprotein substrates
5.1 Materials
5.2 Experimental design
5.3 Results and discussions
5.3.1 Characteristics of substrates and biochar
5.3.2 Daily methane production
5.3.3 Cumulative methane production
5.3.4 Biomethane production potential and biodegradability
5.3.5 Kinetic model evaluation
5.3.6 Anaerobic digestion stability
5.4 Summary
th five-year plan">Chapter 6 Economic factors and cost benefit analysis of AD plants utilizing MSW in Chinaaccording to 13th five-year plan
6.1 Economic factors
6.1.1 Markets
6.1.2 Capitals
6.1.3 Financing modes
6.2 Cost benefit analysis
6.2.1 Investment costs
6.2.2 Operational costs
6.3 Case of a typical BOT funded AD project in China
6.4 Summary
Chapter 7 Conclusions
7.1 Main conclusions
7.2 Innovation
7.3 Research prospects
References
Acknowledgements
List of publications
Introduction of author and supervisor
附件
【參考文獻(xiàn)】:
期刊論文
[1]Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy[J]. Yuan Yue,Zi-Dong Qiu,Xian-You Qu,Ai-Ping Deng,Yuan Yuan,Lu-Qi Huang,Chang-Jiang-Sheng Lai. Journal of Pharmaceutical Analysis. 2018(05)
[2]Effects of free ammonia on volatile fatty acid accumulation and process performance in the anaerobic digestion of two typical bio-wastes[J]. Xuchuan Shi,Jia Lin,Jiane Zuo,Peng Li,Xiaoxia Li,Xianglin Guo. Journal of Environmental Sciences. 2017(05)
本文編號:3108879
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