本文關(guān)鍵詞： 西芹 六次酮層物 黃瓜枯萎病菌 化感作用 化感物質(zhì) 誘導(dǎo)抗性　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本試驗以西芹根物質(zhì)(即西芹鮮根、根際區(qū)物)及西芹種子為材料,利用柱層析法研究了六次酮層物流分對黃瓜枯萎病菌的化感作用,并且通過GC-MS對第六次層析得到的化感作用最佳的流分進(jìn)行物質(zhì)鑒定。結(jié)果表明:1.西芹鮮根第五次層析化感效果最佳的四個流分分別是RA32462,RA33444,RA98898和RA910664,其抑制率與CK對照相比分別提高了 38.74%、38.91%、44.31%、38.17%;第六次層析化感效果最佳的四個流分分別是RA324625,RA334448,RA988981和RA9106645,其抑制率與CK對照相比分別提高了 43.06%、38.56%、45.24%、46.60%。2.西芹根際區(qū)物第五次層析化感效果最佳的四個流分分別是RSA14734,RSA16248,RSA71298和RSA75356,其抑制率與CK對照相比分別提高了 36.35%、30.31%,28.35%、36.12%;第六次層析化感效果最佳的四個流分分別是RSA147341,RSA162489,RSA712982和RSA75356,其抑制率與CK對照相比分別提高了41.00%、38.41%、42.02%、36.38%。3.西芹種子第五次層析化感效果最佳的四個流分分別是SA54838、SA55884、SA714105、SA72263,其抑制率與 CK 對照相比分別提高了 35.18%、48.85%、36.00%、24.96%;第六次層析化感效果最佳的四個流分分別是SA548388、SA558845、SA71410510、SA722635,其抑制率與CK對照相比分別提高了 44.48%、46.58%、36.53%、36.46%。4.第六次層析得到的十二個最佳流分均能誘導(dǎo)黃瓜幼苗對黃瓜枯萎病產(chǎn)生抗性,其中西芹鮮根浸提液四個最佳流分的誘導(dǎo)抗性效果分別達(dá)到82.92%、82.26%、83.53%、80.24%;西芹根際區(qū)物浸提液四個最佳流分的誘導(dǎo)抗性效果分別達(dá)到81.92%、84.61%、84.24%、79.32%;西芹種子浸提液四個最佳流分的誘導(dǎo)抗性效果分別達(dá)到77.58%、80.21%、77.55%、78.29%。誘導(dǎo)試驗表明:西芹鮮根與根際區(qū)物浸提液對黃瓜枯萎病的誘導(dǎo)抗性效果優(yōu)于西芹種子浸提液對黃瓜枯萎病的誘導(dǎo)抗性效果。5.試驗共鑒定出四類十九種具有化感作用的物質(zhì)。其中,烷烴類七種:二十四烷、二十七烷、十八烷、二十八烷、二十一烷、二十三烷、四十四烷;酸類三種:棕櫚酸、十八烷酸、(Z)-9-十八(碳)烯酸;酚類四種:對叔戊基苯酚、對特辛基苯酚、3,5-二叔丁基苯酚、2,6-二叔丁基對甲酚;脂類五種:油酸十八烷醇酯、14-甲基十五烷酸甲酯,棕櫚酸甲酯,鄰苯二甲酸二甲酯、十七酸十六甲基甲基酯。
[Abstract]:In this study, the allelopathic effects of six ketone layer on Fusarium wilt of cucumber were studied by column chromatography using celery root material (that is, celery fresh root, rhizosphere material) and celery seed as materials. The best allelopathy was identified by GC-MS. The results showed that:. 1. The four best allelopathic components of fresh root of celery 5th times were RA32462. Compared with CK, the inhibition rate of RA33444, RA98898 and RA910664 increased 38.74 and 38.91, respectively. The four flow fractions with the best allelopathic effect of 6th chromatography were RA324625ARA334448 and RA988981 and RA9106645, respectively. Compared with CK, the inhibition rate increased by 43.06% and 38.56%, respectively. RSA14734 RSA16248RSA71298 and RSA75356 were the four most sensitive components in the rhizosphere of celery (RSA14734) and RSA71298 (RSA71298). Compared with CK, the inhibition rate increased by 36.35 and 30.31, respectively. The four flow fractions with the best allelopathic effect of 6th chromatography were RSA147341RSA162489, RSA712982 and RSA75356, respectively. Compared with CK, the inhibition rate increased by 41.00% and 38.41%, respectively. The four best allelopathic components of celery seed 5th times were SA54838SSA55884ASA714105S72263. Compared with CK, the inhibition rate increased 35.18% and 48.85%, respectively, and the inhibition rate was 36.00 and 24.96, respectively. The four flow fractions with the best allelopathic effect were SA548388ASA558845, SA71410101010 and SA722635, respectively. Compared with CK, the inhibition rate increased by 44.48% and 46.58%, respectively. The 12 best flow fractions obtained by 36.46. 4.#number0# chromatography could induce cucumber seedlings to develop resistance to Fusarium wilt. The induced resistance of the four best flow fractions of fresh root extract of celery was 82.92 and 82.26, 83.53 and 80.24, respectively. The induced resistance of the four best water fractions in the rhizosphere of celery reached 81.92 and 84.61and 84.2479.32, respectively. The induced resistance of the four best fractions of celery seeds was 77.58% and 80.21%, respectively. 78.29. Induction tests show that:. The induced resistance of fresh root and rhizosphere extract of celery to cucumber wilt was better than that of seed extract of celery to cucumber wilt. 5. Four kinds of 19 allelopathic substances were identified. Among them. Seven alkanes: 24 alkanes, 27 alkanes, 18 alkanes, 28 alkanes, 21 alkanes, 23 alkanes, 44 alkanes; Three kinds of acids: palmitic acid, 18 alkanoic acid, ZC9- 18 (carboxyenoic acid); Four kinds of phenols: p-tert-amylphenol, p-octyl phenol, 3-di-tert-#china_person0# phenol, 2-di-tert-#china_person1# p-cresol; Five kinds of lipids: 18 alkanolyl oleic acid methyl ester 14-methyl 15 alkanoate methyl palmitate dimethyl phthalate 17 acid 16 methyl ester.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S436.421.13

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