【摘要】：目的探討烏頭堿對(duì)新生大鼠心肌細(xì)胞DNA損傷的影響。方法新生SD大鼠20只,隨機(jī)分為4組,取心室肌以差速貼壁法原代培養(yǎng)心室肌細(xì)胞。培養(yǎng)第6天,制成密度為2×105個(gè)細(xì)胞/mL的細(xì)胞懸液,分別加入烏頭堿混合液至終濃度為0.1、0.5、1、2μmol/L,染毒30m in;采用彗星電泳技術(shù)及CASP分析軟件,檢測不同濃度烏頭堿染毒后心室肌細(xì)胞DNA損傷程度。結(jié)果心肌細(xì)胞被不同濃度烏頭堿染毒后,尾部DNA含量、彗尾長度、尾矩、O live尾矩均隨烏頭堿濃度增加而升高,頭部DNA含量則逐漸降低,與對(duì)照組相比,有顯著性差異(P0.01);烏頭堿染毒劑量越大,心肌細(xì)胞DNA損傷越嚴(yán)重。結(jié)論烏頭堿染毒引起細(xì)胞DNA斷裂損傷呈明顯的劑量—效應(yīng)關(guān)系,推測細(xì)胞DNA損傷是烏頭堿毒作用機(jī)制之一。
[Abstract]:Objective to investigate the effect of aconitine on DNA damage in neonatal rat cardiomyocytes. Methods Twenty newborn SD rats were randomly divided into 4 groups. Ventricular myocytes were cultured with differential adhesion method. On the 6th day of culture, cell suspensions with density of 2 脳 105 cells / mL were prepared, and aconitine mixture was added to the final concentration of 0. 1 0. 5 渭 mol/L, for 30 m in;. Comet electrophoresis and CASP software were used to detect the degree of DNA damage in ventricular myocytes exposed to aconitine at different concentrations. Results the contents of DNA, tail length and tail moment of aconitine increased with the increase of aconitine concentration, and the content of DNA in the head decreased gradually, compared with the control group. There was significant difference (P0.01). The greater the dose of aconitine was, the more serious the DNA damage of cardiomyocytes was. Conclusion there is a dose-effect relationship between aconitine and aconitine in the DNA damage induced by aconitine. It is speculated that DNA damage is one of the mechanisms of aconitine toxicity.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院法醫(yī)學(xué)系;湖北省中山醫(yī)院病理科;
【基金】：湖北省自然科學(xué)基金資助項(xiàng)目(2008CDB206)
【分類號(hào)】：D919

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本文編號(hào)：2379684