【摘要】：建立了利用毛細(xì)管電泳光二極管陣列檢測(cè)器連續(xù)波長(zhǎng)掃描分析滾珠水性筆墨水的方法。首先考查了緩沖溶液的類型、濃度、pH值,以及電泳分離電壓對(duì)分析結(jié)果的影響,同時(shí)研究了管徑分別為50,75和100nm的熔融石英毛細(xì)管對(duì)光二極管陣列檢測(cè)器檢測(cè)結(jié)果的影響,實(shí)驗(yàn)表明毛細(xì)管內(nèi)徑的大小與檢測(cè)器的吸光強(qiáng)度成正比,在保證分離效果的前提下,采用管徑為100nm的毛細(xì)管可以提高吸光信號(hào)檢測(cè)強(qiáng)度,因此在紫外和可見光的波長(zhǎng)范圍內(nèi)都可以檢測(cè)到吸收峰。實(shí)驗(yàn)確定濃度為20mmol·L-1、pH值為8.5的四硼酸鈉作為緩沖溶液,熔融石英毛細(xì)管從進(jìn)樣口至檢測(cè)窗口的有效長(zhǎng)度為40cm,內(nèi)徑為100μm,電泳分離電壓為15kV。利用光二極管陣列檢測(cè)器在190～600nm波長(zhǎng)范圍內(nèi)進(jìn)行連續(xù)掃描檢測(cè),根據(jù)三維電泳圖中紫外區(qū)和可見區(qū)電泳吸收峰的特征分析不同種類滾珠水性筆墨水。
[Abstract]:A continuous wavelength scanning method for the analysis of ball pen ink by capillary electrophoresis photodiode array detector was established. The effects of buffer solution type, pH value and electrophoretic separation voltage on the analytical results were investigated, and the effect of fused quartz capillary with diameter of 50 ~ (75) and 100nm on the detection results of photodiode array detector was studied. The experimental results show that the inner diameter of capillary is proportional to the absorptivity of detector. On the premise of ensuring the separation effect, the detection intensity of absorbent signal can be improved by using capillary tube with diameter of 100nm. Therefore, absorption peaks can be detected in the wavelength range of both ultraviolet and visible light. The experimental results show that sodium tetraborate, with concentration of 20mmol L ~ (-1) and pH 8.5, is used as buffer solution. The effective length of fused quartz capillary from the injection port to the detection window is 40 cm, the inner diameter is 100 渭 m, and the electrophoresis separation voltage is 15 kV. The photodiode array detector was used for continuous scanning detection in the 190~600nm wavelength range. According to the characteristics of the absorption peaks in the ultraviolet and visible regions in the three-dimensional electrophoretogram, different kinds of ball pen ink were analyzed.
【作者單位】： 東北師范大學(xué)化學(xué)學(xué)院;中國(guó)刑事警察學(xué)院法化系;中國(guó)人民公安大學(xué);
【基金】：國(guó)家“十一五”科技支撐計(jì)劃項(xiàng)目(2006BAK07B05)資助
【分類號(hào)】：D918.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 李心倩,王彥吉,史曉凡,許英健,王景翰;圓珠筆油墨字跡色痕的高效液相色譜分析方法[J];分析化學(xué);2004年05期

2 王巖,姚麗娟,趙鵬程,王景翰,王彥吉;氣相色譜法確定藍(lán)色圓珠筆油墨字跡的形成時(shí)間[J];色譜;2005年02期

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 史曉凡;李心倩;許英健;王景翰;王彥吉;;高效液相色譜法鑒定藍(lán)色圓珠筆油墨字跡的書寫時(shí)間[J];光譜學(xué)與光譜分析;2006年09期

相關(guān)碩士學(xué)位論文 前2條

1 王秀東;色譜法鑒定藍(lán)色圓珠筆的種類[D];首都師范大學(xué);2006年

2 張淑媛;毛細(xì)管電泳法鑒定圓珠筆油墨[D];首都師范大學(xué);2007年

，

本文編號(hào)：2194986