【摘要】： 目的:探索諾米芬新對亞急性甲基苯丙胺中毒大鼠多巴胺能神經(jīng)系統(tǒng)的保護作用并探討其作用機制。 方法:Wistar雄性大鼠72只,隨機分為生理鹽水組(陰性對照組)、MA組(陽性對照組)和MA、Nomifensine組(保護藥物組)三組。每晚8時分別腹腔注射Nacl 10mg/kg、MA 10mg/kg和MA 10mg/kg加Nom 2mg/kg,連續(xù)四天建立亞急性甲基苯丙胺中毒大鼠模型。通過觀察動物一般情況、刻板行為和CPP實驗,檢驗亞急性甲基苯丙胺中毒大鼠模型建立是否成功。在最后一次給藥后的第一天、第七天和第十四天分別處死各組實驗動物,用免疫組織化學(xué)染色法(S—P法)和熒光分光光度計法從形態(tài)學(xué)、半定量和定量的方法檢測大鼠腦內(nèi)黑質(zhì)、中腦腹側(cè)被蓋區(qū)、前額葉皮質(zhì)、海馬、紋狀體、伏隔核等部位多巴胺神經(jīng)元細胞的形態(tài)和數(shù)量的變化。用圖像分析系統(tǒng)對染色陽性神經(jīng)細胞進行計數(shù)統(tǒng)計,對神經(jīng)纖維進行灰度值分析,將各組數(shù)據(jù)結(jié)果進行比較及統(tǒng)計學(xué)處理。 結(jié)果:1.陰性對照組分別與陽性對照組、保護藥物組在刻板行為評分和持續(xù)時間上比較呈顯著性差異(P＜0.01);保護藥物組和陽性對照組在刻板行為評分和持續(xù)時間上比較呈顯著性差異(P分別為＜0.05和＜0.01);保護藥物組和陽性對照組在CPP實驗結(jié)果上比較呈顯著性差異(P＜0.05)。2.不同腦區(qū)、不同時間點通過對神經(jīng)細胞計數(shù)、神經(jīng)纖維灰度值和TH含量熒光分光光度計測量,結(jié)果顯示保護藥物組神經(jīng)系統(tǒng)損傷輕于陽性對照組。 結(jié)論:諾米芬新對亞急性甲基苯丙胺中毒大鼠多巴胺能神經(jīng)系統(tǒng)具有保護作用。
[Abstract]:Aim: to explore the protective effect of Normifen on dopaminergic nervous system in rats with subacute methamphetamine poisoning and its mechanism. Methods Seventy-two male Wistar rats were randomly divided into three groups: normal saline group (negative control group) and MA-Nomifensine group (positive control group). Nacl 10 mg / kg MA 10mg/kg and MA 10mg/kg plus Nom 2 mg / kg were injected intraperitoneally every night at 8: 00 to establish subacute methamphetamine poisoning rat model for four days. The establishment of subacute methamphetamine poisoning rat model was tested by observing animal general situation, stereotypical behavior and CPP experiment. On the first day, the seventh day and the fourteenth day after the last administration of the drug, the experimental animals were killed, and the morphology of each group was studied by immunohistochemical staining (S-P method) and fluorescence spectrophotometer. The morphologic and quantitative changes of dopamine neurons in substantia nigra ventral tegmental area prefrontal cortex hippocampus striatum and nucleus accumbens were detected by semi-quantitative and quantitative methods. The positive nerve cells were counted and counted by image analysis system, the nerve fibers were analyzed by gray value, the results of each group were compared and statistically processed. The result is 1: 1. Negative control group and positive control group, There were significant differences in the scores and duration of stereotypic behavior between the protective drug group and the positive control group (P < 0.01), while there were significant differences between the protective drug group and the positive control group (P < 0.05 and P < 0.01 respectively). There was a significant difference between the protective drug group and the positive control group in CPP test results (P < 0. 05). 2. The nerve cell count, nerve fiber gray value and th content were measured by fluorescence spectrophotometer in different brain regions and at different time points. The results showed that the nerve system damage in the protective drug group was less than that in the positive control group. Conclusion: Normifen has protective effect on dopaminergic nervous system in rats with subacute methamphetamine poisoning.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2009
【分類號】：D919

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相關(guān)期刊論文 前1條

1 周媛,邢淑華;苯丙胺CPP重現(xiàn)實驗中c-fos和p-CREB在大鼠紋狀體表達的變化[J];中國藥物依賴性雜志;2005年03期

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