本文關(guān)鍵詞：電針對(duì)潰瘍性結(jié)腸炎大鼠腸道微生態(tài)的保護(hù)作用，由筆耕文化傳播整理發(fā)布。

電針對(duì)潰瘍性結(jié)腸炎大鼠腸道微生態(tài)的保護(hù)作用

Effect of Electroacupuncture Intervention on Enteric Microecology in Ulcerative Colitis Rats

[1] [2] [3] [4] [5] [6] [7]

HOU Tian-shu, HAN Xiao-xia , YANG Yang, ZHAO Ji-lan, REN Ya-dong , YU Shu-Guang , WU Qiao- Feng（1 College of Acu-moxibustion and Tuina , Chengdu University of Chinese Medic

[1]成都中醫(yī)藥大學(xué)針灸推拿學(xué)院,成都610075; [2]四川新津縣中醫(yī)院,新津611430; [3]河南鄧州市第三人民醫(yī)院,鄧州474150

文章摘要：目的：觀察電針對(duì)潰瘍性結(jié)腸炎（UC）大鼠腸道菌群的影響，從腸道微生態(tài)的角度探討電針治療UC的機(jī)制。方法：39只健康雄性SD大鼠隨機(jī)分為空白組（13只）和UC造模組（26只），造模組飲用4％的葡聚糖硫酸鈉（DSS）溶液7d復(fù)制UC模型后隨機(jī)分為模型組和電針組。電針“天樞”“足三里”“上巨虛”穴，每日1次，每次10min，治療5d。觀察實(shí)驗(yàn)過(guò)程中大鼠疾病活動(dòng)指數(shù)的變化；收集各組大鼠糞便排泄物，用多聚酶鏈反應(yīng)一變性梯度凝膠電泳法分析腸道菌群的多樣性、相似性及豐度值，對(duì)優(yōu)勢(shì)條帶進(jìn)行回收測(cè)序，采用Quantityone等軟件對(duì)所得結(jié)果圖譜進(jìn)行分析，，對(duì)優(yōu)勢(shì)條帶進(jìn)行菌種鑒定及含量分析。結(jié)果：模型大鼠腸道菌群的豐度值及多樣性指數(shù)明顯降低，疾病活動(dòng)指數(shù)明顯增高（P〈0．05，P〈0．01）。電針能降低UC模型大鼠疾病活動(dòng)指數(shù)，提高腸道菌群的豐度值及多樣性指數(shù)（P〈0．05，P〈0．01）；菌種鑒定表明電針能提高Uc模型大鼠腸道菌群中乳酸桿菌（Lactobacillus sP．）和毛螺科菌（Lachnospiroceae bacterium）含量，降低雙酶梭菌（Clostridiumbifermentans）的含量（P〈0．05）。結(jié)論：電針能明顯改善UC模型大鼠腸道菌群的多樣性以及有益菌群的含量，對(duì)UC模型大鼠腸道微生態(tài)發(fā)揮的保護(hù)作用很可能是電針治療UC的機(jī)制之一。

Abstr：Objective To observe the effect of electroacupuncture （EA） on enteric microbial populations in ulcerative colitis （UC） model rats, so as to study its mechanisms underlying improving UC. Methods Thirty-nine male SD rats were ran- domly divided into control group, model group and EA group （n = 13 in each group）. The UC model was established by intragstric perfusion of 4 % dextran sodiumaqueous solution （DSS）, once daily for 7 days. EA （15- 25 Hz, 0.1 mA） was applied to bilateral “Tianshu” （ST 25）, “Zusanli” （ST 36） and “Shangjuxu” （ST 37） for 10 min, once daily for 5 days. Disease Activity Index （DAD including body weight and stool blood state was used to evaluate the animals’ conditions. The rats＇ excrement was collected to detect the abundance and diversity of bacterial populations by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. Results In comparison with the control group, the animals＇ DAI score and enteric Clostridium bifermentans con- tent were significantly increased （P〈0.05）, and the abundance and diversity of bacterial populations, and contents of enteric Lachnospiraceae bacterium and Lactobacillus sp. were considerably down-regulated in the model group （P〈0. 05, P〈0. 01）. Compared with the model group, the DAI score and enteric Clostridium bifermentans content were significantly lowered, and the abundance and diversity of bacterial populations, and contents of enteric Lachnospiraceae bacterium and Lactobacillus sp. were notably increased in the EA group （P〈0.05, P〈0. 01）. Conclusion EA can improve DAI and the abundance and diversity of enteric bacterial microbiota in UC rats, which may be related to its protective effect on enteric microecology in UC.

文章關(guān)鍵詞：

Keyword：:Ulcerative colitis Electroacupuncture interventions Enteric microecology Polymerase chain reaction-denaturing gradient gel electrophoresis

課題項(xiàng)目：項(xiàng)目來(lái)源：國(guó)家自然科學(xué)基金（No.30901933、81373737）;四川省教育廳重點(diǎn)項(xiàng)目（No.11ZA055）;成都中醫(yī)藥大學(xué)�；穑∟o.ZRYB201101、ZRYY201213）

  本文關(guān)鍵詞：電針對(duì)潰瘍性結(jié)腸炎大鼠腸道微生態(tài)的保護(hù)作用，由筆耕文化傳播整理發(fā)布。