【摘要】：目的：探討海洛因成癮者和冰毒成癮者的外周血白細(xì)胞端粒長度與中醫(yī)證素的關(guān)系,從而在基因水平上探討海洛因和冰毒對人體健康影響的可能中醫(yī)內(nèi)在機(jī)制。方法：研究對象均為年齡在20-49歲的漢族人。實(shí)驗(yàn)組為342例毒品吸食者,其中海洛因吸食者206例(男性129例和女性77例),冰毒吸食者136例(男性80例和女性56例),均為來源于2012年-2014年在福州市公安強(qiáng)制戒毒所和司法廳戒毒管理局所轄的強(qiáng)制戒毒所自愿參加調(diào)查的戒毒人員。對照組為招募自福建省第二人民醫(yī)院體檢中心的200例健康受試者(男性115例和女性85例)。規(guī)范采集四診信息,應(yīng)用證素辨證方法提取中醫(yī)證素特征,運(yùn)用QPCR基礎(chǔ)技術(shù)測量外周血白細(xì)胞的端粒長度。結(jié)果：1.健康組、海洛因組及冰毒組的年齡均為20-49歲,且三組年齡、性別分布無差異(P0.05)。所有參與者的白細(xì)胞端粒長度與年齡、性別呈顯著負(fù)相關(guān)(P0.05)；校正年齡和性別后,海洛因組和冰毒組的端粒長度均比健康組的端粒長度短(P0.05)。2.校正年齡和性別后,海洛因組和冰毒組間端粒長度無差異(P0.05)；兩組端粒長度與吸毒年限均無明顯相關(guān)性(P0.05)；兩組內(nèi)各吸毒年限的端粒長度無明顯差異(P0.05)。3.海洛因和冰毒成癮者的中醫(yī)證素分布大致相同,病位均以肝、脾、腎為主,尤以肝最為多見；病性上,實(shí)證以氣滯最為多見；虛證以氣虛最為多見。4.校正年齡和性別后,觀察端粒長度變化與中醫(yī)證素間的關(guān)系,發(fā)現(xiàn)形成各證素的海洛因組中的端粒長度與健康組的端粒長度均沒有明顯差異(P0.05)；形成肝、濕、熱等證素的冰毒組端粒長度與健康組的端粒長度之間均具有明顯差異(P0.05),且端粒長度比健康組的端粒長度短。5.海洛因組在吸毒年限為4-12年時(shí),20-29歲與30-39歲兩個(gè)年齡段的端粒長度有顯著差異(P0.01),且30-39歲年齡段的端粒長度較20-29歲年齡段的端粒長度縮短；兩年齡段間,肝、脾、腎、氣滯、濕、痰、氣虛、陽虛及血虛等證素的端粒長度有明顯差異(P0.05)。結(jié)論：1.吸食海洛因或吸食冰毒均會(huì)造成吸毒人員外周血白細(xì)胞端粒的損傷,使端粒長度縮短。2.吸食海洛因或吸食冰毒均會(huì)引起臟腑功能失調(diào)及氣血津液損傷,病位主要在肝、腎、脾,尤其以肝的功能失調(diào)最為明顯；氣滯、氣虛是海洛因和冰毒成癮者最突出的病性特點(diǎn)。3.海洛因成癮者在吸毒年限為4-12年,年齡在20-39歲間,其端粒長度變化與其年齡有一定的關(guān)系,即端粒長度可隨年齡增大而縮短,且與肝、脾、腎、氣滯、濕、痰、氣虛、陽虛及血虛等證素的形成有關(guān)。4.冰毒成癮者端粒長度的縮短主要與中醫(yī)肝、濕、熱證素的形成有關(guān)。
[Abstract]:Aim: to explore the relationship between telomere length of peripheral blood leukocytes and TCM syndromes in heroin addicts and meth addicts, so as to explore the possible internal mechanism of the effects of heroin and meth on human health at gene level. Methods: all subjects were Han nationality aged 20 to 49. There were 342 drug addicts in the experimental group, including 206 heroin addicts (M 129 and F 77) and 136 meth users (M 80 and F 56), and the experimental group consisted of 342 drug addicts, including 206 heroin addicts (M 129 and F 77) and 136 meth addicts (M 80 and F 56). All of them were voluntary drug abusers who participated in the investigation from 2012 to 2014 in Fuzhou Public Security compulsory Drug rehab Center and the compulsory Drug Addiction Administration Bureau of the Department of Justice. In the control group, 200 healthy subjects (M 115 and F 85) were recruited from the physical examination Center of the second people's Hospital of Fujian Province. The characteristics of TCM syndrome elements were extracted by syndrome differentiation method, and the telomere length of peripheral blood leukocytes was measured by QPCR basic technique. Results: 1. The age of healthy group, heroin group and meth group were all 20 to 49 years old, and there was no difference in age and sex distribution among the three groups (P0.05). The telomere length of leukocytes in all participants was negatively correlated with age and sex (P0.05). After adjusting for age and sex, the telomere length of heroin group and meth group was shorter than that of healthy group (P0.05). 2. The telomere length of heroin group and meth group was shorter than that of healthy group (P0.05). After adjusting for age and sex, there was no significant difference in telomere length between heroin group and meth group (P0.05), and there was no significant correlation between telomere length and drug use duration between the two groups (P0.05). There was no significant difference in telomere length between the two groups (P0.05). The distribution of TCM syndrome elements in heroin and meth addicts was roughly the same, with liver, spleen and kidney as the main diseases, especially the liver; Qi stagnation was the most common syndrome in disease; Qi deficiency was the most common in deficiency syndrome. 4. After adjusting for age and sex, the relationship between the changes of telomere length and TCM syndromes was observed. There was no significant difference in telomere length between heroin group and healthy group (P0.05). There was a significant difference between the telomere length of the ice group and the healthy group (P0.05), and the telomere length of the meth group was shorter than that of the healthy group (P0.05), and the telomere length of the ice group was shorter than that of the healthy group (P0.05). In heroin group, the telomere length of 20-29-year-old group was significantly different from that of 30-39-year-old group (P0.01), and the telomere length of 30-29-year-old group was shorter than that of 20-29-year-old age group. The telomere length of liver, spleen, kidney, qi stagnation, dampness, phlegm, qi deficiency, yang deficiency and blood deficiency were significantly different between two age groups (P0.05). Conclusion: 1. Heroin or meth can cause telomere damage in peripheral blood leukocytes of drug addicts and shorten telomere length. 2. Heroin or meth can cause dysfunction of Zang-fu organs and injury of qi-blood fluid, especially in liver, kidney and spleen, especially in liver. Stagnation of qi and deficiency of qi are the most prominent disease characteristics of heroin and meth addicts. 3. Heroin addicts take drugs for 4-12 years, aged between 20 and 39, and their telomere length changes have a certain relationship with their age, that is, telomere length can be shortened with age, and with liver, spleen, kidney, qi stagnation, dampness, phlegm, qi deficiency, Yang deficiency and blood deficiency related to the formation of syndromes. 4. The shortening of telomere length in meth addicts is mainly related to the formation of liver, dampness and pyrogen in traditional Chinese medicine.
【學(xué)位授予單位】：福建中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R277.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬蘭;王晶;江濱;劉艷榮;張波;常乃柏;克曉燕;;慢性淋巴細(xì)胞白血病患者單個(gè)核細(xì)胞端粒長度與端粒酶表達(dá)活性研究[J];中國實(shí)驗(yàn)血液學(xué)雜志;2009年06期

2 張寅,平飛云,張行;30例口腔頜面部惡性腫瘤端粒長度變化的研究[J];口腔頜面外科雜志;2002年04期

3 郭令軍;張七一;;實(shí)時(shí)定量PCR分析外周血白細(xì)胞的端粒長度[J];中外醫(yī)療;2008年21期

4 馬曉彩;孫婉玲;;血液系統(tǒng)疾病端粒長度研究進(jìn)展[J];北京醫(yī)學(xué);2013年10期

5 劉利,孫秉中,梁英民,郝淼旺,鄧中榮,張傳山,閻嚴(yán),張方信;急性髓系白血病細(xì)胞端粒酶活性及端粒長度的同步分析[J];中華血液學(xué)雜志;2001年11期

6 姜英華,黃迪南,祝其鋒;端粒長度調(diào)控機(jī)制的研究進(jìn)展[J];腫瘤防治雜志;2004年01期

7 劉義;漢麗梅;潘永榮;金勇;;端粒長度測量方法的研究進(jìn)展[J];世界華人消化雜志;2007年28期

8 林里;李濤平;;阻塞性睡眠呼吸暫停綜合征患者端粒長度的變化及意義[J];南方醫(yī)科大學(xué)學(xué)報(bào);2011年03期

9 商青青;周建業(yè);胡盛壽;;端粒長度的影響因素及其與心血管疾病的關(guān)系[J];中國分子心臟病學(xué)雜志;2012年02期

10 譚善娟;王娜;王威;吳擁軍;吳逸明;;人外周血基因組DNA端粒長度與肺癌關(guān)系[J];中國公共衛(wèi)生;2012年05期

相關(guān)會(huì)議論文 前8條

1 呂昆;林丹;許淼;朱怡文;黃英;;應(yīng)用印跡法測定轉(zhuǎn)基因克隆牛體細(xì)胞端粒長度[A];遺傳學(xué)與社會(huì)可持續(xù)發(fā)展——2010中國青年遺傳學(xué)家論壇論文摘要匯編[C];2010年

2 郝大程;李明霞;肖培根;張宇;陳瑋琦;;定量細(xì)胞端粒長度的實(shí)時(shí)定量PCR方法(英文)[A];2011年中國藥學(xué)大會(huì)暨第11屆中國藥師周論文集[C];2011年

3 葉靜;Christelle Lenain;Serge Bauwen;Arturo Londono;Marie-Joseph Giraud-Panis;吳云林;Eric Gilson;;端粒長度、端粒酶及其相關(guān)蛋白復(fù)合體(Telosome/Shelterin)在胃癌發(fā)生及演進(jìn)中的研究[A];第二十一屆全國中西醫(yī)結(jié)合消化系統(tǒng)疾病學(xué)術(shù)會(huì)議暨國家級(jí)中西醫(yī)結(jié)合消化系統(tǒng)疾病新進(jìn)展學(xué)習(xí)班論文匯編[C];2009年

4 劉瓊;胡德聰;王紅;丁朝建;姜亮;陶德定;徐輝碧;;微量元素鋅、鐵、鉻和鉛對細(xì)胞端粒長度作用的研究[A];中國營養(yǎng)學(xué)會(huì)第八屆微量元素營養(yǎng)學(xué)術(shù)會(huì)議論文摘要匯編[C];2003年

5 劉劍侖;葛蓮英;黎丹戎;張貴年;;大腸癌端粒長度變化與端粒酶活性的研究[A];西部地區(qū)腫瘤學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2004年

6 鞏艷;房殿春;張汝鋼;楊仕明;汪榮泉;彭貴勇;陳文生;羅元輝;;端粒植入對胃癌7901細(xì)胞生長、端粒長度和端粒酶活性和hTERT mRNA表達(dá)的影響[A];中華醫(yī)學(xué)會(huì)第七次全國消化病學(xué)術(shù)會(huì)議論文匯編（下冊）[C];2007年

7 詹昱;易正山;宋蘭林;馮茹;;FLOW-FISH同時(shí)檢測細(xì)胞端粒長度及表面分化抗原方法的建立與優(yōu)化[A];第12屆全國實(shí)驗(yàn)血液學(xué)會(huì)議論文摘要[C];2009年

8 董矜;田亞平;;光照核黃素對腫瘤細(xì)胞生長和端粒長度的作用[A];第6次全國微生物學(xué)與免疫學(xué)大會(huì)論文摘要匯編[C];2004年

相關(guān)重要報(bào)紙文章 前3條

1 新汶;端粒長度較短可預(yù)測卒中后的高癡呆發(fā)生風(fēng)險(xiǎn)[N];醫(yī)藥經(jīng)濟(jì)報(bào);2006年

2 王增;壽命長短可能遺傳于父親[N];衛(wèi)生與生活報(bào);2007年

3 清瀾;科學(xué)家真能倒撥生命時(shí)鐘？[N];北京科技報(bào);2000年

相關(guān)博士學(xué)位論文 前4條

1 曹嵐;端粒的遺傳學(xué)分析及其與肺癌的相關(guān)性研究[D];復(fù)旦大學(xué);2014年

2 賓萍;多環(huán)芳烴暴露對端粒長度的影響及易感性生物標(biāo)志研究[D];中國疾病預(yù)防控制中心;2008年

3 申載薰;芪蓮舒痞顆粒影響端粒酶活性、端粒長度、DNA含量及NF-κB表達(dá)的實(shí)驗(yàn)研究[D];山東中醫(yī)藥大學(xué);2011年

4 賈文文;人端粒酶在小鼠體內(nèi)和iPS細(xì)胞中的表達(dá)調(diào)控[D];西北農(nóng)林科技大學(xué);2011年

相關(guān)碩士學(xué)位論文 前10條

1 趙菲菲;精子端粒長度與IVF過程中早期胚胎發(fā)育的相關(guān)研究[D];鄭州大學(xué);2015年

2 王晗;葉酸對人BJ及A375細(xì)胞株端粒長度和染色體不穩(wěn)定性的影響[D];云南師范大學(xué);2015年

3 馬杰;健康人群外周血白細(xì)胞端粒長度與腎功能關(guān)系的縱向研究[D];吉林大學(xué);2016年

4 于嬌嬌;小鼠鐵過載模型的建立及其對細(xì)胞端粒長度的影響[D];首都醫(yī)科大學(xué);2016年

5 童秋玲;海洛因或冰毒成癮者外周血白細(xì)胞端粒長度與吸毒年限、中醫(yī)證素關(guān)系的研究[D];福建中醫(yī)藥大學(xué);2016年

6 林里;阻塞性睡眠呼吸暫停綜合征患者端粒長度的變化及意義的研究[D];南方醫(yī)科大學(xué);2011年

7 於平;急性創(chuàng)傷對端粒長度的影響及可能機(jī)制的研究[D];上海交通大學(xué);2014年

8 王海霞;正常人及初發(fā)慢性粒細(xì)胞白血病白細(xì)胞中特定染色體相對端粒長度的研究[D];華中科技大學(xué);2010年

9 吳華裕;紅水河流域長壽家系外周血白細(xì)胞染色體端粒長度的研究[D];廣西醫(yī)科大學(xué);2011年

10 王晶;PCR方法檢測端粒長度[D];昆明醫(yī)學(xué)院;2006年

，

本文編號(hào)：2454656