發(fā)布時間：2019-03-27 16:28

【摘要】：目的:探討電針大腸俞募穴治療腸動力異常亢進(jìn)的作用機(jī)制。方法:60只大鼠隨機(jī)分為對照組、模型組、俞募組、俞穴組、募穴組,每組12只。采用番瀉葉煎劑灌胃造模法,同日電針刺激天樞、大腸俞,每次20min,共治療10次。運用光鏡、HE染色、免疫組化等技術(shù),觀察電針治療后大鼠排便情況、海馬及腸道組織細(xì)胞形態(tài)學(xué)改變、海馬組織中BDNF蛋白表達(dá)水平。結(jié)果:腸動力亢進(jìn)模型建立后,與對照組比較,1 h排便糞粒數(shù)、24 h排便糞�？倲�(shù)、24 h糞便含水量均顯著增高(P0.01),海馬CA2區(qū)、CA3區(qū)、DG區(qū)BDNF蛋白表達(dá)水平顯著減少,以DG區(qū)最為明顯(P0.01);治療后,募穴組大鼠的1 h排便糞粒數(shù)、募穴組和俞募組大鼠的24 h排便糞�？倲�(shù)和24 h糞便含水量較模型組顯著降低(P0.01),俞募組、募穴組、俞穴組上述腦區(qū)中BDNF蛋白表達(dá)水平顯著回升,以DG區(qū)最為明顯(P0.01);光鏡下模型組、針刺組大鼠海馬、腸道組織形態(tài)均有所改變,針刺組改變程度較輕。結(jié)論:大腸俞募穴可以改善腸動力亢進(jìn)大鼠的異常排便情況,通過提高海馬DG區(qū)BDNF蛋白表達(dá)水平而發(fā)揮中樞調(diào)控作用可能是其起效的一個重要機(jī)制。
[Abstract]:Objective: to explore the mechanism of electro-acupuncture (EA) at large intestine Shu (EA) for treatment of abnormal hyperactivity of intestinal motility. Methods: 60 rats were randomly divided into three groups: control group, model group, Yu exixie group, Yu acupoint group and Baixue point group, with 12 rats in each group. The model was made by using Senna leaf decoction by gavage. Electroacupuncture was used to stimulate Tianshu and large intestine on the same day for 20 minutes each time for 10 times. By means of light microscopy, HE staining and immunohistochemistry, the defecation, morphological changes of hippocampal and intestinal tissue cells and the expression of BDNF protein in hippocampus were observed after electroacupuncture treatment in rats. Results: compared with the control group, the number of feces, the total number of feces at 24 h and the water content of feces at 24 h after the establishment of the model were significantly higher than those in the control group (P0.01). The number of fecal granules in hippocampal CA2 area and CA3 area were significantly higher than those in the control group (P0.01). The expression level of BDNF protein in DG region was significantly decreased, especially in DG region (P0.01). After treatment, the number of fecal particles at 1 hour in group A, the total number of fecal particles in 24 h and water content in 24 h in group A and group Yu were significantly lower than those in the model group (P0.01), and those in group Yu and Yu were significantly lower than those in group A (P 0.01), and the number of fecal particles in group A was significantly lower than that in group A. The expression level of BDNF protein in the above brain regions increased significantly in the Yu-acupoint group, especially in the DG area (P0.01). The histological changes of hippocampus and intestinal tract of rats in model group and acupuncture group were all changed under light microscope, and the degree of change in acupuncture group was less severe than that in control group. Conclusion: Jiaoshu Xizhi point can improve the abnormal defecation of rats with intestinal motility hyperkinesia, and the central regulatory role may be an important mechanism of its effect by increasing the expression of BDNF protein in the DG region of hippocampus.
【作者單位】： 陜西中醫(yī)藥大學(xué)針灸推拿學(xué)院;成都中醫(yī)藥大學(xué)針灸推拿學(xué)院;天津中醫(yī)藥大學(xué)第一附屬醫(yī)院;
【基金】：國家自然科學(xué)基金面上項目(81473777) 成都中醫(yī)藥大學(xué)研究生創(chuàng)新基金項目(2016)
【分類號】：R245

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