【摘要】：目的:研究回藥核心方油溶液對(duì)離體大鼠胸主動(dòng)脈血管環(huán)的舒張作用及機(jī)制,為其用于心血管疾病的治療提供參考。方法:取出大鼠胸主動(dòng)脈血管環(huán)后浸泡于克氏營(yíng)養(yǎng)液(K-H)中,以1×10~(-6)mol/L去甲腎上腺素(PE)或60 mmol/L氯化鉀(KCl)致血管環(huán)收縮,采用生物信號(hào)采集分析系統(tǒng)測(cè)定0.020 4、0.040 8、0.061 2、0.081 6、0.102 0 mg/mL核心方油溶液對(duì)血管環(huán)的舒張作用,計(jì)算舒張率;分別以0.1 mmol/L一氧化氮合酶抑制劑左旋硝基精氨酸甲酯(L-NAME)、環(huán)氧合酶抑制劑吲哚美辛(INDO)、鉀離子通道阻滯劑格列本脲(Gli)孵育血管環(huán)20 min后,測(cè)定上述5種質(zhì)量濃度核心方油溶液對(duì)PE預(yù)收縮血管環(huán)的舒張作用,計(jì)算舒張率;試驗(yàn)均以K-H溶液為空白對(duì)照。結(jié)果:與空白對(duì)照比較,0.020 4~0.102 0 mg/mL核心方油溶液對(duì)PE、KCl預(yù)收縮血管環(huán)均有明顯的舒張作用(P0.05或P0.01),且具有濃度依賴性。INDO預(yù)處理后可減弱核心方油溶液對(duì)PE預(yù)收縮血管環(huán)的舒張作用,舒張率較空白對(duì)照組差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);而Gli、L-NAME預(yù)處理不影響核心方油溶液對(duì)PE預(yù)收縮血管環(huán)的舒張作用,舒張率較空白對(duì)照組仍明顯升高(P0.05或P0.01)。結(jié)論:核心方油溶液可呈濃度依賴性地舒張PE、KCl預(yù)收縮的胸主動(dòng)脈血管環(huán),其作用機(jī)制可能與激活環(huán)氧合酶途徑有關(guān)。
[Abstract]:Aim: to study the vasodilation of isolated rat thoracic aortic rings and its mechanism in order to provide reference for the treatment of cardiovascular diseases. Methods: rat thoracic aortic rings were taken out and immersed in Kjh solution. The rings were induced by 1 脳 10 ~ (- 6) mol/L norepinephrine (PE) or 60 mmol/L potassium chloride (KCl) (KCl _ 2), and the vasoconstriction was induced by 1 脳 10 ~ (- 6) mmol/L noradrenaline (PE). The vasodilation effects of 0.020, 0.0408,0.061,200, 0.0816, 0.102 mg/mL core oil solution on vascular rings were measured by biological signal acquisition and analysis system, and the relaxation rate was calculated. The vascular rings were incubated with 0.1 mmol/L nitric oxide synthase inhibitor L-nitro arginine methyl ester (L-NAME) and indomethacin (INDO), potassium channel blocker glibenclamide (Gli) for 20 min, respectively. The vasodilation effect of the core oil solution of the above five concentrations on the pre-constrictive vascular rings of PE was measured and the relaxation rate was calculated. K H solution was used as the blank control. Results: compared with the blank control, the 0.020 mg/mL core oil solution had obvious vasodilation effect on the pre-constricted vascular rings of PE,KCl (P0.05 or P0.01), and there was no significant difference between the control group and the control group (P0.05 or P0.01). Indo pretreatment could attenuate the vasodilation effect of core oil solution on PE pre-contraction, and there was no significant difference in diastolic rate compared with the blank control group (P0.05). Pretreatment with Gli,L-NAME did not affect the vasodilation effect of the core oil solution on the pre-contraction of PE, and the diastolic rate was still significantly higher than that of the blank control group (P0.05 or P0.01). Conclusion: the core oil solution can dilate the thoracic aortic rings precontracted by PE,KCl in a concentration-dependent manner, the mechanism of which may be related to the activation of cyclooxygenase pathway.
【作者單位】： 寧夏醫(yī)科大學(xué)總醫(yī)院藥劑科;寧夏醫(yī)科大學(xué)藥學(xué)院;寧夏回藥現(xiàn)代化工程技術(shù)研究中心;寧夏回醫(yī)藥協(xié)同創(chuàng)新中心;寧夏醫(yī)科大學(xué)回醫(yī)藥現(xiàn)代化省部共建教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家科技支撐計(jì)劃課題(No.2013BAI11B07) 寧夏回族自治區(qū)科技攻關(guān)計(jì)劃項(xiàng)目(No.2012) 大學(xué)生創(chuàng)新/創(chuàng)業(yè)計(jì)劃項(xiàng)目(No.201510752008)
【分類號(hào)】：R29
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本文編號(hào)：2435966