【摘要】：目的:觀察槲皮素脂質(zhì)體(LQ)對糖尿病大鼠腎臟糖基化終產(chǎn)物(AGEs)及其受體(RAGE)和TGF-β1表達的影響。方法:采用旋轉(zhuǎn)蒸發(fā)法制備槲皮素脂質(zhì)體,高糖高脂飼料聯(lián)合腹腔注射鏈脲佐菌素(STZ)建立2型糖尿病大鼠模型,并隨機分為糖尿病模型組(DM組),槲皮素脂質(zhì)體低(LQ-L)、中(LQ-M)、高(LQ-H)劑量組,氨基胍(AG)對照組(AG組),另設(shè)正常組(N組)。灌胃治療8周后測定各組大鼠血糖、體質(zhì)量、腎臟肥大指數(shù)(KI)、血尿素氮(BUN)、血肌酐(Scr)、血清中超氧化物歧化酶(SOD)、丙二醛(MDA),ELISA法測血清AGEs表達和24 h尿微量白蛋白,PAS染色觀察腎臟病理改變,免疫組化測腎組織AGEs和TGF-β1表達,RT-PCR檢測腎皮質(zhì)RAGE mRNA和TGF-β1 mRNA表達水平。結(jié)果:與N組比較,DM組大鼠血糖、KI、BUN、Scr、MDA含量、血清AGEs和24 h尿微量白蛋白顯著升高,血清中SOD活性水平、體質(zhì)量明顯降低;腎小球體積萎縮,基底膜增厚;腎組織AGEs、TGF-β1和RAGE mRNA、TGF-β1 mRNA表達增高。與DM組比較,LQ各劑量組大鼠血糖、KI、BUN、Scr、MDA含量、血清AGEs和24 h尿微量白蛋白均降低,血清中SOD活性水平、體質(zhì)量增加;病理改變明顯減輕;腎組織AGEs、TGF-β1和RAGE m RNA、TGF-β1 mRNA表達降低,以中劑量組作用更明顯。結(jié)論:LQ可抑制蛋白質(zhì)非酶糖基化反應(yīng)和細胞外基質(zhì)(ECM)增生,從而減少腎組織AGEs、TGF-β1生成及RAGE mRNA、TGF-β1 mRNA表達水平,對糖尿病大鼠腎臟具有保護作用。
[Abstract]:Aim: to observe the effect of quercetin liposome (LQ) on the expression of (AGEs), its receptors (RAGE) and TGF- 尾 1 in diabetic rats. Methods: quercetin liposomes were prepared by rotating evaporation. The rats with type 2 diabetes were induced by high glucose and high fat diet and intraperitoneal injection of streptozotocin (STZ). The rats were randomly divided into diabetic model group (DM group). Quercetin liposome (LQ-L), moderate (LQ-M), high dose (LQ-H) group, aminoguanidine (AG) control group (AG group), and normal group (N group). Blood glucose, body mass, renal hypertrophy index (KI), blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD),) and malondialdehyde (SOD),) (MDA), in rats were measured after 8 weeks of gastric perfusion. The expression of serum AGEs and 24 h urinary microalbumin were measured by ELISA method, the pathological changes of kidney were observed by PAS staining, the expressions of AGEs and TGF- 尾 1 in renal tissues were detected by immunohistochemistry, and the expressions of RAGE mRNA and TGF- 尾 1 mRNA in renal cortex were detected by RT-PCR. Results: compared with group N, the contents of blood glucose, KI,BUN,Scr,MDA, serum AGEs and 24 h urinary microalbumin in DM group were significantly increased, the activity of SOD in serum and body weight were significantly decreased, glomerular volume atrophy and basement membrane thickening were observed. The expression of AGEs,TGF- 尾 1 and RAGE mRNA,TGF- 尾 1 mRNA was increased in renal tissue. Compared with DM group, the levels of blood glucose, KI,BUN,Scr,MDA, serum AGEs and 24 h urinary microalbumin decreased, the activity of SOD in serum and body mass increased, and the pathological changes were alleviated. The expression of AGEs,TGF- 尾 1 and RAGE m RNA,TGF- 尾 1 mRNA in renal tissue was decreased, especially in middle dose group. Conclusion: LQ can inhibit the protein non-enzymatic glycosylation reaction and the proliferation of extracellular matrix (ECM), thus reduce the production of AGEs,TGF- 尾 1 and the expression of RAGE mRNA,TGF- 尾 1 mRNA in renal tissue. It has protective effect on the kidney of diabetic rats.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R259

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