IL-4等炎癥因子與登革熱中醫(yī)證型的相關(guān)性研究
發(fā)布時間：2019-02-15 09:43
【摘要】：目的：本課題將普通登革熱患者急性期血清中6種細胞因子和中醫(yī)的證型相結(jié)合,觀察不同中醫(yī)證型細胞因子的表達水平,從中研究兩者是否存在關(guān)聯(lián)性,使西醫(yī)的診斷和微觀指標與中醫(yī)的辨證論治相結(jié)合,形成登革熱獨特的中西醫(yī)結(jié)合診療規(guī)律,為其辨證分型提供客觀依據(jù),并篩選出有提示意義的指標,有助于提高中醫(yī)藥在登革熱的診治和防控領(lǐng)域的應(yīng)用,使登革熱在中醫(yī)臨床上的辨證分型和診治更加可靠和準確。方法：利用廣州中醫(yī)藥大學第一附屬醫(yī)院的電子病歷系統(tǒng),對登革熱住院病人的基本資料和中醫(yī)證型進行檢索搜集,從中隨機選取衛(wèi)氣同病型患者41例(A組)、濕熱遏阻型患者41例(B組)和20例健康者(C組)。實驗采用免疫學酶聯(lián)免疫吸附法(ELISA方法)檢測各組樣本血清中炎癥細胞因子IL-4、IL-12、IL-17、IFN-γ、TNF-α和TGF-β1的水平,將登革熱中醫(yī)的衛(wèi)氣同病證、濕熱遏阻證和健康對照組的結(jié)果進行統(tǒng)計學分析。結(jié)果：(1)IL-4在濕熱遏阻組與健康對照組之間有差異,P=0.0430.05,且濕熱遏阻組高于健康對照組。(2)IL-12在衛(wèi)氣同病組與健康對照組之間有顯著性差異,P=0.0000.01,且衛(wèi)氣同病組高于健康對照組；IL-12在濕熱遏阻組與健康對照組之間有顯著性差異,P=0.0000.01,且濕熱遏阻組高于健康對照組。(3)IFN-γ在衛(wèi)氣同病組與健康對照組之間有顯著性差異,P=-0.0050.01,且衛(wèi)氣同病組高于健康對照組；IFN-γ在濕熱遏阻組與健康對照組之間有顯著性差異,P=0.0380.05,且濕熱遏阻組高于健康對照組。(4)登革熱組內(nèi)IL-4與IL-12、IL-17、IFN-γ都存在顯著性正相關(guān)(f0.01),IL-4與TGF-β1存在正相關(guān)(P0.05)；IL-12與IL-17、IFN-γ存在顯著正相關(guān)(P0.01); IL-17與IFN-γ存在顯著正相關(guān)(P0.01)。結(jié)論：(1)登革熱衛(wèi)氣同病證患者血清IL-12和IFN-γ比健康對照組明顯升高。(2)登革熱濕熱遏阻證患者血清IL-4、IL-12和IFN-γ比健康對照組明顯升高。(3)登革熱患者存在著炎癥細胞因子的過度激活,并與中醫(yī)證型密切相關(guān),提示炎癥細胞因子可作為登革熱中醫(yī)辨證分型的重要客觀化指標。(4)登革熱細胞因子之間的相關(guān)性分析發(fā)現(xiàn),炎癥細胞因子的釋放在炎癥反應(yīng)過程中存在相互促進的作用。
[Abstract]:Objective: to observe the expression level of cytokines in different TCM syndromes by combining six cytokines in the serum of patients with dengue fever in the acute phase, and to investigate whether there is a correlation between the cytokines of different TCM syndromes. To combine the diagnosis and micro index of western medicine with the differentiation and treatment of traditional Chinese medicine, to form the unique law of diagnosis and treatment of dengue fever, to provide objective basis for the differentiation and classification of symptoms and signs, and to screen out the indicators of significance for the diagnosis and treatment of dengue fever. It is helpful to improve the application of traditional Chinese medicine in the field of diagnosis and control of dengue fever and to make the clinical diagnosis and treatment of dengue fever more reliable and accurate. Methods: using the electronic medical record system of the first affiliated Hospital of Guangzhou University of traditional Chinese Medicine, the basic data and TCM syndromes of inpatients with dengue fever were collected. 41 patients with the same disease of Wei Qi were randomly selected from the patients (group A). There were 41 cases of dampness and heat suppression type (group B) and 20 cases of healthy persons (group C). The levels of inflammatory cytokines IL-4,IL-12,IL-17,IFN- 緯, TNF- 偽 and TGF- 尾 1 in serum of each group were detected by immunological enzyme linked immunosorbent assay (ELISA method). The results of dampness and heat suppression syndrome and healthy control group were statistically analyzed. Results: (1) there was significant difference in IL-4 between damp-heat suppression group and healthy control group (P < 0.05), and the IL-12 of dampness and heat suppression group was higher than that of healthy control group. (2) there was a significant difference in IL-12 between Weiqi group and healthy control group. P0. 000 0. 01, and Wei Qi with disease group is higher than the healthy control group; There was a significant difference in IL-12 between damp-heat suppression group and healthy control group (P0. 0000. 01). (3) there was a significant difference in IFN- 緯 between Weiqi group and healthy control group, and the IFN- 緯 level of dampness and heat suppression group was higher than that of healthy control group. The patients in the same disease group were higher than those in the healthy control group. There was a significant difference in IFN- 緯 between damp-heat suppression group and healthy control group, which was 0.038 0.05, and higher in damp-heat suppression group than in healthy control group. (4) IL-4 and IL-12,IL-17, in dengue fever group were significantly higher than those in healthy control group. There was a positive correlation between IFN- 緯 and TGF- 尾 1 (f0.01), and a positive correlation between IL-4 and TGF- 尾 1 (P0.05). There was a significant positive correlation between IL-12 and IL-17,IFN- 緯 (P0.01), and between IL-17 and IFN- 緯 (P0.01). Conclusion: (1) the serum IL-12 and IFN- 緯 of the patients with the same syndrome of dengue qi and qi were significantly higher than those of the healthy control group. (2) the serum IL-4, of the patients with dampness and heat suppression syndrome of dengue fever was significantly increased. IL-12 and IFN- 緯 were significantly higher than those in healthy controls. (3) there was excessive activation of inflammatory cytokines in patients with dengue fever, which was closely related to TCM syndromes. The results suggest that inflammatory cytokines can be used as an important objective index in the differentiation and classification of dengue fever syndrome. (4) the correlation analysis between the cytokines of dengue fever revealed that the release of inflammatory cytokines played a mutually reinforcing role in the process of inflammation.
【學位授予單位】：廣州中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R259
本文編號：2423207
[Abstract]:Objective: to observe the expression level of cytokines in different TCM syndromes by combining six cytokines in the serum of patients with dengue fever in the acute phase, and to investigate whether there is a correlation between the cytokines of different TCM syndromes. To combine the diagnosis and micro index of western medicine with the differentiation and treatment of traditional Chinese medicine, to form the unique law of diagnosis and treatment of dengue fever, to provide objective basis for the differentiation and classification of symptoms and signs, and to screen out the indicators of significance for the diagnosis and treatment of dengue fever. It is helpful to improve the application of traditional Chinese medicine in the field of diagnosis and control of dengue fever and to make the clinical diagnosis and treatment of dengue fever more reliable and accurate. Methods: using the electronic medical record system of the first affiliated Hospital of Guangzhou University of traditional Chinese Medicine, the basic data and TCM syndromes of inpatients with dengue fever were collected. 41 patients with the same disease of Wei Qi were randomly selected from the patients (group A). There were 41 cases of dampness and heat suppression type (group B) and 20 cases of healthy persons (group C). The levels of inflammatory cytokines IL-4,IL-12,IL-17,IFN- 緯, TNF- 偽 and TGF- 尾 1 in serum of each group were detected by immunological enzyme linked immunosorbent assay (ELISA method). The results of dampness and heat suppression syndrome and healthy control group were statistically analyzed. Results: (1) there was significant difference in IL-4 between damp-heat suppression group and healthy control group (P < 0.05), and the IL-12 of dampness and heat suppression group was higher than that of healthy control group. (2) there was a significant difference in IL-12 between Weiqi group and healthy control group. P0. 000 0. 01, and Wei Qi with disease group is higher than the healthy control group; There was a significant difference in IL-12 between damp-heat suppression group and healthy control group (P0. 0000. 01). (3) there was a significant difference in IFN- 緯 between Weiqi group and healthy control group, and the IFN- 緯 level of dampness and heat suppression group was higher than that of healthy control group. The patients in the same disease group were higher than those in the healthy control group. There was a significant difference in IFN- 緯 between damp-heat suppression group and healthy control group, which was 0.038 0.05, and higher in damp-heat suppression group than in healthy control group. (4) IL-4 and IL-12,IL-17, in dengue fever group were significantly higher than those in healthy control group. There was a positive correlation between IFN- 緯 and TGF- 尾 1 (f0.01), and a positive correlation between IL-4 and TGF- 尾 1 (P0.05). There was a significant positive correlation between IL-12 and IL-17,IFN- 緯 (P0.01), and between IL-17 and IFN- 緯 (P0.01). Conclusion: (1) the serum IL-12 and IFN- 緯 of the patients with the same syndrome of dengue qi and qi were significantly higher than those of the healthy control group. (2) the serum IL-4, of the patients with dampness and heat suppression syndrome of dengue fever was significantly increased. IL-12 and IFN- 緯 were significantly higher than those in healthy controls. (3) there was excessive activation of inflammatory cytokines in patients with dengue fever, which was closely related to TCM syndromes. The results suggest that inflammatory cytokines can be used as an important objective index in the differentiation and classification of dengue fever syndrome. (4) the correlation analysis between the cytokines of dengue fever revealed that the release of inflammatory cytokines played a mutually reinforcing role in the process of inflammation.
【學位授予單位】：廣州中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R259
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