基于DNA條形碼技術(shù)的維吾爾藥材桑葚基原鑒定研究
發(fā)布時間：2019-01-19 08:46
【摘要】：該研究通過分析多途徑來源的新疆桑屬植物及藥材樣本的ITS2,psbA-trnH序列,為藥材分子鑒定提供依據(jù)。以51個新疆桑屬植物及藥材為樣本,對其ITS2,psbA-trnH序列進(jìn)行PCR擴增和測序,用MEGA 6.0計算其種內(nèi)、種間Kimura 2-parameter(K2P)距離,分析變異位點,并構(gòu)建NJ鑒別樹。ITS2序列分析結(jié)果顯示,桑Morus alba、韃靼桑M.alba var.tatarica、黑桑M.nigra種內(nèi)無變異;桑與韃靼桑種間無變異;桑與黑桑種間存在13個變異位點,種間平均KP2遺傳距離為0.04;桑與藥材樣本間無信息變異位點,NJ鑒別樹可將桑及韃靼桑與黑桑區(qū)分。psbA-trnH序列分析結(jié)果顯示,桑與黑桑種內(nèi)各有1個變異位點,3種植物種間存在插入/缺失變異,可相互區(qū)分;種間變異與藥材樣本內(nèi)變異一致。因此,ITS2序列可將來源于桑、韃靼桑的藥材樣本與黑桑區(qū)分,psbA-trnH序列可將來源于三者的藥材樣本區(qū)分,為維吾爾藥材真?zhèn)舞b別及市場監(jiān)管提供依據(jù)。
[Abstract]:In this study, the ITS2,psbA-trnH sequences of mulberry plants and their samples from various sources were analyzed to provide basis for molecular identification of medicinal materials. The ITS2,psbA-trnH sequences of 51 mulberry plants and medicinal materials from Xinjiang were amplified by PCR and sequenced. The distance of Kimura 2-parameter (K2P) between species and species was calculated by MEGA 6.0, and the variation sites were analyzed. NJ identification tree was constructed. The results of ITS2 sequence analysis showed that there was no variation in the M.nigra species of Morus alba, Tatar mulberry M.alba var.tatarica,. There were 13 variation sites between mulberry and black mulberry, and the average genetic distance of KP2 between mulberry and black mulberry was 0.04. There were no information mutation sites between mulberry and medicinal material samples, and NJ discriminant tree could distinguish mulberry and Tartar mulberry from black mulberry. The results of psbA-trnH sequence analysis showed that there was one mutation locus in mulberry and black mulberry species, and there was insertion / deletion mutation among planting species. Distinguishable from each other; The interspecific variation was consistent with the variation in the samples of medicinal materials. Therefore, ITS2 sequence can distinguish the samples from mulberry, Tartar mulberry and black mulberry, psbA-trnH sequence can distinguish the samples from the three, and provide the basis for the identification and market supervision of Uygur medicinal materials.
【作者單位】： 新疆維吾爾自治區(qū)中藥民族藥研究所國家中醫(yī)藥管理局新疆中藥民族藥資源重點實驗室;新疆出入境檢驗檢疫局檢驗檢疫技術(shù)中心;
【基金】：新疆維吾爾自治區(qū)科技廳公益項目(KY2015127) 國家質(zhì)量監(jiān)督檢驗檢疫總局科技計劃項目(2016IK262)
【分類號】：R29
，
本文編號：2411196
[Abstract]:In this study, the ITS2,psbA-trnH sequences of mulberry plants and their samples from various sources were analyzed to provide basis for molecular identification of medicinal materials. The ITS2,psbA-trnH sequences of 51 mulberry plants and medicinal materials from Xinjiang were amplified by PCR and sequenced. The distance of Kimura 2-parameter (K2P) between species and species was calculated by MEGA 6.0, and the variation sites were analyzed. NJ identification tree was constructed. The results of ITS2 sequence analysis showed that there was no variation in the M.nigra species of Morus alba, Tatar mulberry M.alba var.tatarica,. There were 13 variation sites between mulberry and black mulberry, and the average genetic distance of KP2 between mulberry and black mulberry was 0.04. There were no information mutation sites between mulberry and medicinal material samples, and NJ discriminant tree could distinguish mulberry and Tartar mulberry from black mulberry. The results of psbA-trnH sequence analysis showed that there was one mutation locus in mulberry and black mulberry species, and there was insertion / deletion mutation among planting species. Distinguishable from each other; The interspecific variation was consistent with the variation in the samples of medicinal materials. Therefore, ITS2 sequence can distinguish the samples from mulberry, Tartar mulberry and black mulberry, psbA-trnH sequence can distinguish the samples from the three, and provide the basis for the identification and market supervision of Uygur medicinal materials.
【作者單位】： 新疆維吾爾自治區(qū)中藥民族藥研究所國家中醫(yī)藥管理局新疆中藥民族藥資源重點實驗室;新疆出入境檢驗檢疫局檢驗檢疫技術(shù)中心;
【基金】：新疆維吾爾自治區(qū)科技廳公益項目(KY2015127) 國家質(zhì)量監(jiān)督檢驗檢疫總局科技計劃項目(2016IK262)
【分類號】：R29
，
本文編號：2411196
本文鏈接：http://sikaile.net/zhongyixuelunwen/2411196.html
最近更新
教材專著
