【摘要】：目的:本文通過(guò)實(shí)驗(yàn)對(duì)藏藥莪達(dá)夏不同部位體外和體內(nèi)化合物信息及其在大鼠體內(nèi)可能的移行變化進(jìn)行探討研究。方法:通過(guò)醇提,萃取等方法提取得到藏藥莪達(dá)夏乙酸乙酯部位、氯仿部位、水部位和正丁醇部位,應(yīng)用LC-MS技術(shù)檢測(cè)分析其化學(xué)成分,確定其化合物信息;將已適應(yīng)飼養(yǎng)7天的大鼠隨機(jī)分為4組:分別為乙酸乙酯部位組(EAEO)、氯仿部位組(CHEO)、正丁醇部位組(ZDCO)、水部位組(EDX-S),每組6只,各組大鼠禁食12小時(shí)后,分別于末次灌胃0、30、60、90、120、180、240、360、480、720、1 440 min眼眶靜脈取血0.5 mL,應(yīng)用LC-MS技術(shù)檢測(cè)分析其化學(xué)成分,確定化合物信息,研究其在大鼠血漿中的移行變化;末次采血后采集大鼠肝臟和腸內(nèi)糞便樣品,進(jìn)行肝微粒體轉(zhuǎn)化實(shí)驗(yàn)和腸內(nèi)轉(zhuǎn)化實(shí)驗(yàn),分別應(yīng)用LC-MS技術(shù)檢測(cè)分析其化學(xué)成分,確定化合物信息,研究其在大鼠肝臟和腸內(nèi)的移行變化。結(jié)果:(1)通過(guò)LC-MS檢測(cè),得到莪達(dá)夏不同部位入血化學(xué)成分,其中:莪達(dá)夏氯仿部位有15個(gè)化學(xué)成分(包括12個(gè)黃酮類化合物,1個(gè)糖苷類化合物,2個(gè)其它類化合物);乙酸乙酯部位有18個(gè)化學(xué)成分(包括13個(gè)黃酮類化合物,1個(gè)酰胺類化合物,1個(gè)糖苷類化合物,3個(gè)其它類化合物);水部位有40個(gè)化學(xué)成分(包括24個(gè)黃酮類成分,2個(gè)酰胺類化合物,1個(gè)糖苷類化合物,13個(gè)其它類化合物);正丁醇部位有14個(gè)化學(xué)成分(包括10個(gè)黃酮類化合物,1個(gè)酰胺類化合物,3個(gè)其它類化合物)。(2)莪達(dá)夏不同部位中的一些黃酮類化合物(包括7-羥基黃酮,白楊素,黃芹素,槲皮素,7-甲氧基黃酮,鼠李素,2’-羥基-4’-甲氧基查爾酮,7-羥基二氫黃酮,2’,4’-二羥基查耳酮,染料木黃酮)可能在大鼠血漿中參與一系列生化反應(yīng),參與的多為Ⅰ相反應(yīng)(包括羥基化反應(yīng)和甲基化反應(yīng)等)。(3)莪達(dá)夏不同部位中的一些黃酮類化合物(包括楊梅素,2’,4’-二羥基查爾酮,3’,4’,5,7-四羥基雙氫黃酮)可能參與大鼠一系列肝臟代謝反應(yīng),參與的多為Ⅰ相反應(yīng)(包括羥基化反應(yīng)和甲基化反應(yīng)等)。(4)莪達(dá)夏不同部位中的一些黃酮類化合物(包括槲皮素,7-甲氧基黃酮,7-羥基二氫黃酮,楊梅素)可能在大鼠體內(nèi)參與一系列腸道代謝過(guò)程,參與的多為Ⅰ相反應(yīng)(包括羥基化反應(yīng)和甲基化反應(yīng)等)。結(jié)論:1.莪達(dá)夏不同部位中參與體內(nèi)代謝過(guò)程的化合物主要是黃酮類化合物。因此,認(rèn)為總黃酮可能是莪達(dá)夏在大鼠體內(nèi)發(fā)揮其藥理和藥效作用的主要有效成分。2.藏藥莪達(dá)夏中黃酮類化合物參與體內(nèi)代謝的途徑可能主要是Ⅰ相反應(yīng),包括甲基化和羥基化反應(yīng)等。3.參與體內(nèi)代謝的黃酮類化合物包括楊梅素,白楊素,黃芹素,槲皮素,鼠李素,染料木黃酮,7-羥基黃酮,7-甲氧基黃酮,7-羥基二氫黃酮,2’,4’-二羥基查耳酮,2’-羥基-4’-甲氧基查爾酮和3’,4’,5,7-四羥基雙氫黃酮。
[Abstract]:Aim: to study the information of compounds in different parts of Tibetan medicine Erdaxia in vitro and in vivo and their possible transference changes in rats. Methods: the components of ethyl acetate, chloroform, water and n-butanol of Tibetan medicine were extracted by alcohol extraction and extraction. The chemical constituents were detected and analyzed by LC-MS technique, and the information of their compounds was determined. The rats who had been fed for 7 days were randomly divided into 4 groups: ethyl acetate group, (EAEO), chloroform fraction group, (CHEO), n-butanol fraction group, (ZDCO), water fraction group (EDX-S), 6 rats in each group. The rats in each group were fasted for 12 hours. The chemical constituents were detected and analyzed by LC-MS for 0.5 mL, after the last intragastric infusion of the blood collected from the orbital vein at the last time. The chemical constituents were determined and the changes of the compounds in the plasma of the rats were studied by using the LC-MS technique. The results were as follows: (1) the changes of the chemical composition in the plasma of the rats were determined by using the LC-MS technique, and the chemical constituents were determined by the LC-MS technique. The changes of the chemical constituents in the plasma were studied. After the last blood collection, liver and intestinal fecal samples were collected, liver microsomal transformation test and enteral transformation test were carried out. The chemical constituents were detected and analyzed by LC-MS technique, and the compound information was determined. To study the changes of liver and intestine migration in rats. Results: (1) the chemical constituents of different parts of Edaraxia were obtained by LC-MS detection. Among them, there were 15 chemical constituents (including 12 flavonoids, 1 glycoside) in the chloroform of Edaraxia. 2 other compounds); There are 18 chemical constituents in ethyl acetate (including 13 flavonoids, 1 amide, 1 glycoside and 3 other compounds). There were 40 chemical constituents (including 24 flavonoids, 2 amides, 1 glycoside and 13 other compounds). There are 14 chemical constituents of n-butanol (including 10 flavonoids, 1 amides, 3 other compounds). (2). Yellow apigenin, quercetin, 7-methoxyflavone, rohamnetin, 2-hydroxy-4- methoxy chalcone, 7- hydroxy dihydroflavone, 2-dihydroxy chalone, Genistein may be involved in a series of biochemical reactions in rat plasma, mostly in phase I reactions (including hydroxylation reaction and methylation reaction), and some flavonoids (including myricetin) in different parts of Erdaxia. (2) it may be involved in a series of metabolic reactions in the liver of rats. Some flavonoids (including quercetin, 7-methoxyflavone, 7-hydroxy dihydroflavone, etc.) from different parts of Erdasha were involved in phase 鈪，

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