朝醫(yī)三神散對血管性癡呆大鼠學(xué)習(xí)記憶及細(xì)胞凋亡的實驗研究
發(fā)布時間：2018-12-17 19:30
【摘要】：目的：1.探究朝醫(yī)三神散對血管性癡呆大鼠(VaD)學(xué)習(xí)以及記憶能力和空間探索能力的影響；2.探究朝醫(yī)三神散對血管性癡呆大鼠大腦海馬體的細(xì)胞形狀、排列的影響；3.探究朝醫(yī)三神散及對血管性癡呆大鼠大腦海馬體CAI區(qū)神經(jīng)元細(xì)胞超微結(jié)構(gòu)、細(xì)胞凋亡的影響。方法：取SD大鼠40只,雌雄各半,按雌雄隨機(jī)分為假手術(shù)組、模型組、喜得鎮(zhèn)對照組(西藥組)、朝醫(yī)三神散中劑量組、朝醫(yī)三神散高劑量組。采用雙側(cè)頸總動脈動脈夾閉制備血管性癡呆(VaD)大鼠模型,除了假手術(shù)組外,均予注射硝普鈉及進(jìn)行雙側(cè)頸動脈夾閉,手術(shù)結(jié)束后第二天開始每天定時給予上述五組灌胃,連續(xù)28天,藥品名字及劑量如下：假手術(shù)組予蒸餾水10ml/kg、模型組予蒸餾水10ml/kg、喜得鎮(zhèn)對照組(西藥組)予喜得鎮(zhèn)溶液0.6mg/kg、朝醫(yī)三神散中劑量組予朝醫(yī)三神散9g/kg、朝醫(yī)三神散高劑量組給予朝醫(yī)三神散18g/kg灌胃,灌胃最后五天應(yīng)用morris水迷宮檢測血管性癡呆大鼠治療前后行為學(xué)改變；后予灌流取腦制作石蠟切片,應(yīng)用HE染色觀察海馬CA1區(qū)神經(jīng)元的結(jié)構(gòu)形態(tài)改變并對神經(jīng)元數(shù)目進(jìn)行定量分析。應(yīng)用原位末端標(biāo)記法(TUNEL)檢測細(xì)胞凋亡。結(jié)果：1.經(jīng)過Morris水迷宮實驗研究表明,假手術(shù)組與模型組相比在學(xué)習(xí)記憶能力及空間探索能力均有統(tǒng)計學(xué)意義(P0.05)。模型組與喜得鎮(zhèn)組、朝醫(yī)三神散高劑量組均有統(tǒng)計學(xué)意義(P0.05)。朝醫(yī)三神散中劑量組與模型組在水迷宮學(xué)習(xí)記憶能力實驗中第一天和第四天有統(tǒng)計學(xué)意義(P0.05),喜得鎮(zhèn)組與朝醫(yī)三神散高劑量組、朝醫(yī)三神散中劑量組之間無統(tǒng)計學(xué)意義(P0.05)2.經(jīng)過HE染色可見,假手術(shù)組海馬CA1區(qū)細(xì)胞大部分為橢圓形,排列整齊,大小一致,模型組海馬CA1區(qū)細(xì)胞排列紊亂,形態(tài)各異,喜得鎮(zhèn)組、朝醫(yī)三神散高劑量組、朝醫(yī)三神散中劑量組與模型組相比較,細(xì)胞排列較模型組整齊,橢圓形大小細(xì)胞較模型組多見。3.經(jīng)過TUNEL染色及海馬CA1區(qū)細(xì)胞凋亡計數(shù),假手術(shù)組與模型組相比,具有統(tǒng)計學(xué)意義(P0.05),模型組與喜得鎮(zhèn)組、朝醫(yī)三神散高劑量組、朝醫(yī)三神散中劑量組相比均有統(tǒng)計學(xué)意義(P0.05),喜得鎮(zhèn)組、朝醫(yī)三神散高劑量組、朝醫(yī)三神散中劑量組之間無統(tǒng)計學(xué)意義(P0.05)。結(jié)論：1.朝醫(yī)三神散對血管性癡呆大鼠學(xué)習(xí)記憶有改善作用,2.朝醫(yī)三神散能夠改善血管性癡呆大鼠細(xì)胞的結(jié)構(gòu)和形態(tài),3.朝醫(yī)三神散對血管性癡呆大鼠海馬體CAI區(qū)神經(jīng)元細(xì)胞的凋亡有部分恢復(fù)作用。
[Abstract]:Objective: 1. To explore the effect of Chaoyi San Shen San on (VaD) learning, memory ability and spatial exploration ability in vascular dementia rats. 2. To explore the effect of Chaoyi San Shen San on the shape and arrangement of hippocampal cells in rats with vascular dementia. To explore the effect of Chaoyi San Shen San on the ultrastructure and apoptosis of neurons in CAI region of cerebral hippocampus of vascular dementia rats. Methods: forty SD rats, male and female, were randomly divided into sham-operation group, model group, Xidazen control group (western medicine group), middle dose group of Chaoyi Sanshen powder and high dose group of Chaoyi Sanshen powder. The (VaD) rat model of vascular dementia was established by bilateral common carotid artery occlusion. Except for sham operation group, sodium nitroprusside was injected and bilateral carotid artery occlusion was performed. For 28 consecutive days, the drug name and dosage were as follows: sham operation group was given 10 ml / kg distilled water, model group was treated with distilled water 10 ml / kg, and the control group (western medicine group) was given 0.6 mg / kg Xide solution. The middle dose group was given 9 g / kg Chaoyi Sanshen Powder, and the high dose group was given 18g/kg. The behavioral changes of vascular dementia rats before and after treatment were detected by morris water maze on the last five days. The brain was then perfused to make paraffin sections. The structural and morphological changes of neurons in the hippocampal CA1 region were observed by HE staining and the number of neurons was quantitatively analyzed. Apoptosis was detected by in situ end labeling (TUNEL). Results: 1. Morris water maze experiment showed that the sham-operation group compared with the model group in learning and memory ability and spatial exploration ability were statistically significant (P0.05). Model group and Xidazhen group, Chaoyi Sanshen high dose group were statistically significant (P0.05). The middle dose group and model group had statistical significance in the first day and the fourth day of the experiment of learning and memory ability of water maze (P0.05), the high dose group of Xide Zhen group and the high dose group of Chaoyi Sanshen Powder (P0.05), and the model group had statistical significance in the experiment of learning and memory ability of water maze (P0.05). There was no significant difference between the two groups (P0.05). HE staining showed that most of the CA1 cells in the hippocampus of sham-operation group were oval, neatly arranged and uniform in size. In the model group, the cells in the CA1 area of hippocampus were disordered and varied in shape, in the Xidazhen group, and in the high dose group of Chaoyi Sanshen Powder. Compared with the model group, the cells were arranged neatly in the middle dose group of Chaoyi Sanshen Powder, and the oval cells were more than those in the model group. After TUNEL staining and apoptosis count in hippocampal CA1 area, there was significant difference between sham operation group and model group (P0.05). There was no significant difference between the middle dose group (P0.05), the high dose group (P 0.05), the high dose group (P 0.05) and the middle dose group (P 0.05). Conclusion: 1. Chaoyi San Shen San can improve the learning and memory of vascular dementia rats, 2. Chaoyi San Shen San can improve the structure and morphology of vascular dementia rats. Chaoyi San Shen San had partial recovery effect on apoptosis of CAI neurons in hippocampus of vascular dementia rats.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29
本文編號：2384698
[Abstract]:Objective: 1. To explore the effect of Chaoyi San Shen San on (VaD) learning, memory ability and spatial exploration ability in vascular dementia rats. 2. To explore the effect of Chaoyi San Shen San on the shape and arrangement of hippocampal cells in rats with vascular dementia. To explore the effect of Chaoyi San Shen San on the ultrastructure and apoptosis of neurons in CAI region of cerebral hippocampus of vascular dementia rats. Methods: forty SD rats, male and female, were randomly divided into sham-operation group, model group, Xidazen control group (western medicine group), middle dose group of Chaoyi Sanshen powder and high dose group of Chaoyi Sanshen powder. The (VaD) rat model of vascular dementia was established by bilateral common carotid artery occlusion. Except for sham operation group, sodium nitroprusside was injected and bilateral carotid artery occlusion was performed. For 28 consecutive days, the drug name and dosage were as follows: sham operation group was given 10 ml / kg distilled water, model group was treated with distilled water 10 ml / kg, and the control group (western medicine group) was given 0.6 mg / kg Xide solution. The middle dose group was given 9 g / kg Chaoyi Sanshen Powder, and the high dose group was given 18g/kg. The behavioral changes of vascular dementia rats before and after treatment were detected by morris water maze on the last five days. The brain was then perfused to make paraffin sections. The structural and morphological changes of neurons in the hippocampal CA1 region were observed by HE staining and the number of neurons was quantitatively analyzed. Apoptosis was detected by in situ end labeling (TUNEL). Results: 1. Morris water maze experiment showed that the sham-operation group compared with the model group in learning and memory ability and spatial exploration ability were statistically significant (P0.05). Model group and Xidazhen group, Chaoyi Sanshen high dose group were statistically significant (P0.05). The middle dose group and model group had statistical significance in the first day and the fourth day of the experiment of learning and memory ability of water maze (P0.05), the high dose group of Xide Zhen group and the high dose group of Chaoyi Sanshen Powder (P0.05), and the model group had statistical significance in the experiment of learning and memory ability of water maze (P0.05). There was no significant difference between the two groups (P0.05). HE staining showed that most of the CA1 cells in the hippocampus of sham-operation group were oval, neatly arranged and uniform in size. In the model group, the cells in the CA1 area of hippocampus were disordered and varied in shape, in the Xidazhen group, and in the high dose group of Chaoyi Sanshen Powder. Compared with the model group, the cells were arranged neatly in the middle dose group of Chaoyi Sanshen Powder, and the oval cells were more than those in the model group. After TUNEL staining and apoptosis count in hippocampal CA1 area, there was significant difference between sham operation group and model group (P0.05). There was no significant difference between the middle dose group (P0.05), the high dose group (P 0.05), the high dose group (P 0.05) and the middle dose group (P 0.05). Conclusion: 1. Chaoyi San Shen San can improve the learning and memory of vascular dementia rats, 2. Chaoyi San Shen San can improve the structure and morphology of vascular dementia rats. Chaoyi San Shen San had partial recovery effect on apoptosis of CAI neurons in hippocampus of vascular dementia rats.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29
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