【摘要】：目的:通過(guò)觀察電針對(duì)甲狀腺區(qū)切口痛大鼠頸背根神經(jīng)節(jié)(DRG)內(nèi)衛(wèi)星膠質(zhì)細(xì)胞(SGCs)活性的影響,探討針刺緩解術(shù)后痛或針刺鎮(zhèn)痛行甲狀腺手術(shù)的機(jī)制。方法:SD大鼠隨機(jī)分為正常組、模型組、扶突組、合谷-內(nèi)關(guān)組、足三里-陽(yáng)陵泉組,每組20只。除正常組外,沿大鼠頸正中線做一縱行切口并反復(fù)分離刺激,制作甲狀腺區(qū)切口痛模型。各治療組分別電針雙側(cè)"扶突""合谷-內(nèi)關(guān)""足三里-陽(yáng)陵泉"穴,1次/d,連續(xù)3d。檢測(cè)大鼠切口部位熱痛閾;用免疫熒光、Real-time PCR、ELISA和蛋白免疫印跡法分別檢測(cè)大鼠脊髓頸(C)2-C 6DRGs內(nèi)SGCs標(biāo)記物膠質(zhì)纖維酸性蛋白(GFAP)、connexin 43(Cx 43)免疫熒光強(qiáng)度和白細(xì)胞介素-1β(IL-1β)、腫瘤壞死因子-α(TNF-α)、IL-6mRNA和蛋白表達(dá)量以及Cx 43蛋白表達(dá)量。結(jié)果 :模型組大鼠頸部的熱痛閾較正常組明顯縮短(P0.05);扶突組和合谷-內(nèi)關(guān)組大鼠熱痛閾均較模型組和足三里-陽(yáng)陵泉組顯著延長(zhǎng)(P0.05)。DRGs內(nèi)GFAP免疫熒光強(qiáng)度及mRNA表達(dá)水平與IL-1β、TNF-α、IL-6mRNA和蛋白表達(dá)量及Cx 43免疫熒光強(qiáng)度與蛋白表達(dá)量在模型組均明顯上調(diào)(P0.05);與模型組比較,電針雙側(cè)"扶突""合谷-內(nèi)關(guān)"穴后GFAP免疫熒光強(qiáng)度及mRNA表達(dá)水平與IL-1β、TNF-α、IL-6mRNA表達(dá)水平和Cx 43免疫熒光強(qiáng)度與蛋白表達(dá)量,扶突組IL-1β、TNF-α與合谷-內(nèi)關(guān)組IL-6蛋白表達(dá)量均明顯下調(diào)(P0.05),足三里-陽(yáng)陵泉組除IL-1β、TNF-αmRNA外,多數(shù)指標(biāo)變化不明顯(P0.05)。結(jié)論:電針"扶突""合谷-內(nèi)關(guān)"穴可緩解大鼠頸部切口痛,該作用可能與其下調(diào)C 2-C 6DRGs內(nèi)SGCs活性,減少促炎細(xì)胞因子的釋放,減弱SGCs間信息交流密切相關(guān)。
[Abstract]:Objective: to observe the effect of electroacupuncture on (SGCs) activity of satellite glia in (DRG) of cervical dorsal root ganglion (DRG) of rats with incision pain in thyroid region, and to explore the mechanism of relieving postoperative pain or acupuncture analgesia by acupuncture. Methods: SD rats were randomly divided into normal group, model group, FUP group, Hegu-Neiguan group and Zusanli-Yanglingquan group with 20 rats in each group. In addition to the normal group, a longitudinal incision was made along the median line of the neck of the rat and stimulated repeatedly to make the model of incision pain in the thyroid region. Each treatment group was treated with electroacupuncture at Zusanli Yangling Quan (Zusanli-Yanglingquan) once a day for 3 days. The thermal pain threshold of the incision was measured in rats. Detection of glial fibrillary acidic protein (GFAP), in rat spinal cord (C) 2-C 6DRGs by immunofluorescence, Real-time PCR,ELISA and Western blot Connexin 43 (Cx 43) immunofluorescence intensity, interleukin-1 尾 (IL-1 尾), tumor necrosis factor- 偽 (TNF- 偽), IL-6mRNA and protein expression and Cx 43 protein expression. Results: the thermal pain threshold of the model group was significantly shorter than that of the normal group (P0.05). Compared with model group and Zusanli-Yanglingquan group, the thermal pain threshold in FUP group and Hegu Neiguan group was significantly longer than that in model group and Zusanli-Yanglingquan group (P0.05) GFAP immunofluorescence intensity and mRNA expression level and IL-1 尾, TNF- 偽 were significantly increased in FUP group and Hegu Neiguan group. The expression of IL-6mRNA and protein, the immunofluorescence intensity and protein expression of Cx 43 were significantly up-regulated in the model group (P0.05). Compared with the model group, GFAP immunofluorescence intensity and mRNA expression level, IL-1 尾, TNF- 偽, IL-6mRNA expression level and Cx 43 immunofluorescence intensity and protein expression level after electroacupuncture at Hegu and Neiguan acupoints of both sides were compared, IL-1 尾, IL-1 尾 in FUT group were significantly higher than those in control group. The expression of TNF- 偽 and IL-6 protein in Hegu-Neiguan group were significantly down-regulated (P0.05), except IL-1 尾 and TNF- 偽 mRNA in Zusanli-Yanglingquan group (P0.05). Conclusion: electroacupuncture at Hegu Neiguan point can relieve the pain of neck incision in rats, which may be related to its down-regulation of SGCs activity in C2-C 6DRGs, the reduction of inflammatory cytokines release and the decrease of information exchange among SGCs.
【作者單位】： 中國(guó)中醫(yī)科學(xué)院針灸研究所;長(zhǎng)春中醫(yī)藥大學(xué)針灸推拿學(xué)院;北京中醫(yī)藥大學(xué)針灸推拿學(xué)院;
【基金】：國(guó)家自然科學(xué)基金青年基金項(xiàng)目(No.81303055);國(guó)家自然科學(xué)基金面上項(xiàng)目(No.81273833) 科技部國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃“973”項(xiàng)目(No.2014CB 543103、2013CB 531904) 中國(guó)中醫(yī)科學(xué)院針灸研究所科研業(yè)務(wù)費(fèi)自主選題(No.ZZ 201711006)
【分類號(hào)】：R245.97

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