【摘要】：目的:通過建立慢性應(yīng)激條件下的哮喘大鼠模型,從分子水平研究不同濃度疏肝理肺方對哮喘大鼠血清皮質(zhì)酮(CORT)及肺組織MMP-9表達(dá)的影響,進(jìn)一步探索疏肝理肺方控制慢性應(yīng)激哮喘氣道重塑的機(jī)制。方法:48只健康雌性SD大鼠隨機(jī)分為6組:正常組(A組)、應(yīng)激模型組(B組)、地塞米松組(C組)、疏肝理肺方低濃度組(D組)、疏肝理肺方中濃度組(E組)、疏肝理肺方高濃度組(F組),每組8只。除正常組外,余各組采用氫氧化鋁-卵清蛋白(OVA)致敏激發(fā)哮喘,同時(shí)加用慢性不可預(yù)見性溫和應(yīng)激法建立慢性應(yīng)激模型。造模結(jié)束后取材,HE染色觀察肺組織病理學(xué)改變,ELISA法檢測血清CORT的含量,Western blot法測定肺組織MMP-9的表達(dá)。結(jié)果:與A組相比,B組肺組織炎性細(xì)胞明顯浸潤,支氣管平滑肌及基底膜增厚,粘膜腫脹,褶皺形成,與A組相比,B組支氣管壁面積和氣道平滑肌面積顯著增高(P0.05);與B組相比較,治療組上述癥狀明顯減輕(P0.05);與E組相比,D、F組上述癥狀減輕不明顯(P0.05)。Western和ELISA結(jié)果顯示,C組和疏肝理肺方各濃度組上述表現(xiàn)較應(yīng)激模型組減輕;疏肝理肺方低濃度組和高濃度組上述表現(xiàn)較中濃度組增強(qiáng)。B組CORT和MMP-9表達(dá)量較A組明顯升高(P0.05);治療組CORT和MMP-9表達(dá)量較B組降低(P0.05);D組和F組CORT和MMP-9表達(dá)量較C組和E組高(P0.05)。結(jié)論:疏肝理肺方可通過抑制血清CORT及肺組織MMP-9的表達(dá),有效緩解慢性應(yīng)激條件下的哮喘大鼠氣道重塑。
[Abstract]:Objective: to study the effects of Shugan Lifei recipe (Shugan Lifei) on the expression of corticosterone (CORT) in serum and MMP-9 in lung tissue of asthmatic rats at molecular level by establishing a rat model of asthma under chronic stress. To further explore the mechanism of Shugan Lifei recipe to control airway remodeling in chronic stress asthma. Methods: 48 healthy female SD rats were randomly divided into 6 groups: normal group (group A), stress model group (group B), dexamethasone group (group C), low concentration group of Shugan Lifei prescription (group D), middle concentration group of Shugan lifei prescription (group E) and high concentration of Shugan Lifei prescription. Group F, 8 rats in each group. In addition to the normal group, the other groups were sensitized by aluminum hydroxide ovalbumin (OVA) to stimulate asthma, and the chronic stress model was established by chronic unpredictable mild stress. The pathological changes of lung tissue were observed by HE staining and the expression of MMP-9 in lung tissue was detected by, Western blot method and the content of serum CORT by ELISA method. Results: compared with group A, pulmonary inflammatory cells in group B were significantly infiltrated, bronchial smooth muscle and basement membrane thickened, mucosal swelling and fold formation. Compared with group A, the area of bronchial wall and airway smooth muscle in group B was significantly higher than that in group A (P0.05), and that in group B was higher than that in group B (P0.05). Compared with E group, the above symptoms in group D and F were not significantly alleviated (P0.05). Western and ELISA results showed that the above symptoms in group C and Shugan Lifei group were lighter than those in stress model group. The expression of CORT and MMP-9 in group B was significantly higher than that in group A (P0.05); the expression of CORT and MMP-9 in treatment group was lower than that in group B (P0.05). The expression of CORT and MMP-9 in group F and); D were higher than those in group C and E (P0.05). Conclusion: Shuganlifei decoction can effectively relieve airway remodeling of asthmatic rats under chronic stress by inhibiting the expression of serum CORT and lung tissue MMP-9.
【學(xué)位授予單位】：桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R259

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