【摘要】：目的:對藏木香進行生藥學和質量標準制訂研究。方法:對藏木香藥材的基原、性狀、理化鑒別、水分、總灰分及酸不溶性灰分和醇溶性浸出物進行檢測。采用0.25%硝酸銀溶液制備的硅膠G薄層板,以土木香內酯與異土木香內酯為對照,建立藏木香的薄層鑒別方法;采用Welchrom C_(18)色譜柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸溶液(55∶45)為流動相,在210 nm波長下對藏木香中的土木香內酯和異土木香內酯進行定量分析。結果:藏木香的TLC特征斑點清晰,分離度好;土木香內酯的進樣量在0.416~4.16μg(r1=0.9999)、異土木香內酯在0.402~4.02μg(r2=0.9998)范圍內與各峰面積值呈良好線性關系;異土木香內酯的平均加樣回收率為100.49%,RSD為1.79%(n=6),土木香內酯的平均加樣回收率為98.85%,RSD為1.21%(n=6)。結論:10批藏木香藥材中異土木香內酯和土木香內酯的平均質量分數(shù)分別為2.379%、2.067%,水分為9.93%~11.46%,總灰分為3.28%~4.99%,酸不溶性灰分為0.35%~0.67%,醇浸出物為56.42%~71.32%。該研究所建立的方法操作簡便、結果準確、重現(xiàn)性好,可有效控制藏木香的質量。
[Abstract]:Objective: to study the pharmacognosy and quality standard of Radix Cassiae. Methods: the primordium, characters, physical and chemical identification, moisture, total ash, acid insoluble ash and alcohol soluble extract were determined. Using silica gel G thin layer plate prepared by 0.25% silver nitrate solution, the thin layer identification method of Tibetan wood was established by using the method of Welchrom C _ (18) column (250 mm 脳 4.6 mm,5 渭 m),) and acetonitrile-0.1% phosphoric acid solution (55:45) as mobile phase. The quantitative analysis of soilactone and isopodiactone in Tibetan wood was carried out at the wavelength of 210 nm. Results: the TLC characteristic spots were clear and the degree of separation was good, and the injection amount of coriolide was 0.416 ~ 4.16 渭 g (r ~ (1) ~ (0.9999), and the range of isophora lactone was 0.402 ~ 4.02 渭 g (r ~ (2) ~ (2) ~ (99) ~ (9). The average recovery of isomaractone was 100.49% (RSD = 1.79%), and the average recovery was 98.85% (RSD = 1.21%). Conclusion: the average mass fractions of isomaractone and coriolactone in 10 batches of Chinese herbal medicine were 2.379 and 2.0677.The water was divided into 9.93 and 11.46 and the total ash was 3.280.99. the acid insoluble ash was 0.350.67, and the alcohol extract was 56.42C71.32. The established method is simple, accurate and reproducible, and can be used to control the quality of Xanthoxylum amurensis.
【作者單位】： 四川大學華西醫(yī)院臨床藥學部;四川大學華西藥學院;
【分類號】：R29

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