【摘要】：目的探討瑤藥小鉆乙酸乙酯提取物抗類風濕關節(jié)炎(rheumatoid arthritis,RA)作用及相關機制。方法取雄性SD大鼠70只,隨機分為正常對照組、模型對照組、雷公藤組、地塞米松組、小鉆乙酸乙酯提取物低、中、高劑量組。建立膠原性關節(jié)炎(collagen induced arthritis,CIA)大鼠模型,連續(xù)灌胃給藥28 d,觀察大鼠狀態(tài)、體重、足腫脹度、關節(jié)炎指數(shù)、踝關節(jié)病變;采用ELISA法測定血清白細胞介素-1β(IL-1β)、白細胞介素-6(IL-6)及腫瘤壞死因子-α(TNF-α)含量;采用實時熒光定量PCR法檢測關節(jié)滑膜組織NF-κB p65 mRNA表達。結果小鉆乙酸乙酯提取物能顯著抑制CIA大鼠足腫脹,降低血清IL-1β、IL-6、TNF-α及滑膜組織NF-κB p65 mRNA水平,作用強于雷公藤,弱于地塞米松,與劑量相關。結論小鉆有一定抗RA作用,其機制可能與NF-κB p65轉錄有關。
[Abstract]:Objective to investigate the anti-rheumatoid arthritis (rheumatoid arthritis,RA) effect of ethyl acetate extract from Chinese herbal medicine. Methods 70 male SD rats were randomly divided into normal control group, model control group, Tripterygium wilfordii group, dexamethasone group and low, middle and high dose groups. The rat model of collagen-induced arthritis (collagen induced arthritis,CIA) was established. The rats were administrated intragastrically for 28 days. The state, body weight, foot swelling, arthritis index and ankle lesion were observed. The levels of serum interleukin-1 尾 (IL-1 尾), interleukin-6 (IL-6) and tumor necrosis factor- 偽 (TNF- 偽) were measured by ELISA assay, and the expression of NF- 魏 B p65 mRNA in synovial tissue was detected by real-time fluorescence quantitative PCR method. Results the ethyl acetate extract significantly inhibited foot swelling in CIA rats, and decreased the levels of serum IL-1 尾, IL-6,TNF- 偽 and NF- 魏 B p65 mRNA in synovial tissue, which was stronger than Tripterygium wilfordii and weaker than that of dexamethasone in dose-dependent manner. Conclusion small drill has some anti-RA effect, and its mechanism may be related to the transcription of NF- 魏 B p65.
【作者單位】： 廣西衛(wèi)生職業(yè)技術學院生理教研室;廣西藥用植物研究所廣西藥用資源保護與遺傳改良重點實驗室;
【基金】：國家自然科學基金資助項目(81660721) 廣西教育廳高�？蒲匈Y助項目(KY2015YB461) 廣西衛(wèi)計委自籌經費資助項目(Z2015442)
【分類號】：R29

【相似文獻】

相關期刊論文 前1條

1 古蘭·托來西;依巴代提·吐乎提;胡君萍;艾斯木提拉·奴爾艾合買提;艾尼瓦爾·吾買爾;;維吾爾藥羅勒乙酸乙酯提取物抗炎作用的研究[J];海峽藥學;2011年02期

相關碩士學位論文 前2條

1 李光;蘇木乙酸乙酯提取物對動脈粥樣硬化模型大鼠血清Hs-CRP的影響[D];黑龍江中醫(yī)藥大學;2013年

2 黃光浩;蘇木乙酸乙酯提取物對動脈粥樣硬化模型大鼠血清白細胞介素6（IL-6）的影響[D];黑龍江中醫(yī)藥大學;2013年

，

本文編號：2266053