【摘要】：目的:觀察艾灸聽宮對膜迷路積水模型大鼠耳蝸形態(tài)、血漿血管加壓素(arginine vasopressin,AVP)濃度及耳蝸水通道蛋白2(aquaporin2,AQP2)表達(dá)的影響,探討艾灸聽宮對膜迷路積水的可能調(diào)節(jié)機(jī)制。方法:將28只Wistar大鼠隨機(jī)分為空白對照組,模型對照組,托伐普坦組及艾灸聽宮組,每組7只。除外空白對照組,其余各組均采用腹腔注射AVP的方法復(fù)制膜迷路積水模型。空白對照組與模型對照組采取與艾灸聽宮組相同的固定,不予治療;托伐普坦組予托伐普坦溶液5 mg/kg灌胃;艾灸聽宮組于聽宮穴施灸20 min。各治療組均每日治療1次,連續(xù)治療10 d。干預(yù)結(jié)束后,行HE染色法觀察大鼠耳蝸積水程度,計算蝸管橫截面積與蝸管加前庭階橫截面積之和的比值(R值);放免法檢測血漿AVP濃度;免疫組化法檢測耳蝸血管紋AQP2蛋白的表達(dá)水平。結(jié)果:(1)模型對照組出現(xiàn)膜迷路積水,前庭膜向前庭階方向不同程度的隆起擴(kuò)張,R值較空白對照組增加(P0.01);托伐普坦組與艾灸聽宮組前庭膜隆起程度均較模型對照組不同程度的減輕,R值均較模型對照組降低(P0.01,P0.05);艾灸聽宮組R值與托伐普坦組比較,差異無統(tǒng)計學(xué)意義(P0.05)。(2)模型對照組大鼠血漿AVP濃度較空白對照組升高(P0.01);托伐普坦組與艾灸聽宮組血漿AVP濃度均較模型對照組降低(均P0.05);艾灸聽宮組血漿AVP濃度與托伐普坦組比較,差異無統(tǒng)計學(xué)意義(P0.05)。(3)模型對照組耳蝸血管紋AQP2表達(dá)水平較空白對照組升高(P0.01);托伐普坦組與艾灸聽宮組AQP2表達(dá)水平均較模型對照組降低(均P0.01);艾灸聽宮組AQP2表達(dá)水平與托伐普坦組比較,差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:艾灸聽宮可減輕大鼠膜迷路積水,其作用機(jī)制可能與對AVP-AQP2系統(tǒng)的調(diào)節(jié)有關(guān)。
[Abstract]:Objective: to observe the effects of moxibustion on cochlea morphology, plasma vasopressin (arginine vasopressin,AVP) concentration and cochlear aquaporin-2 (aquaporin2,AQP2) expression in rats with membranous labyrinth hydrops, and to explore the possible regulatory mechanism of moxibustion on membranous labyrinthine hydrops. Methods: Twenty-eight Wistar rats were randomly divided into three groups: blank control group, model control group, Tovaptan group and moxibustion auricular group with 7 rats in each group. Except the blank control group, the other groups were treated by intraperitoneal injection of AVP to establish the model of hydrolabyrinth. The blank control group and the model control group were treated with the same fixation as the moxibustion group without treatment; the torphaptan group was given tovastam solution for 5 mg/kg; the moxibustion group was treated with moxibustion at the hearing point for 20 min. All the treatment groups were treated once a day for 10 days. After the intervention, the degree of cochlea hydrops in rats was observed by HE staining, the ratio of cochlear duct cross-sectional area to cochlear duct plus vestibular cross sectional area (R value) was calculated, the plasma AVP concentration was detected by radioimmunoassay. The expression of AQP2 protein in stria vascularis of cochlea was detected by immunohistochemical method. Results: (1) the membrane labyrinth hydrops appeared in the model control group. The R value of vestibular membrane to vestibular step was higher than that of blank control group (P0.01), and the vestibular membrane bulge degree of Tofaptan group and moxibustion auricular temple group was lower than that of model control group (P0.01), and the R value of vestibular membrane bulge in Tofaptan group and moxibustion aegong group was lower than that in model control group. The R value of moxibustion group was higher than that of torphoptan group, while that of moxibustion group was lower than that of Tofaptan group. There was no significant difference (P0.05). (2). The plasma AVP concentration in the model group was higher than that in the blank control group (P0.01); the plasma AVP concentration in the Tofaptan group and moxibustion auricular group was lower than that in the model control group (P0.05); the plasma AVP concentration in the moxibustion hearing palace group was higher than that in the Topaptan group. There was no significant difference (P0.05). (3) in the expression of AQP2 in the cochlear stria of the model control group compared with the blank control group (P0.01), the level of AQP2 expression in the Tofaptan group and the moxibustion auricular group was lower than that in the model control group (P0.01), and the expression of AQP2 in the moxibustion group was lower than that in the model control group (P0.01). Compared with the Tofaptan formation, The difference was not statistically significant (P0.05). Conclusion: moxibustion can relieve the membranous labyrinthine hydrops in rats, and its mechanism may be related to the regulation of AVP-AQP2 system.
【作者單位】： 浙江中醫(yī)藥大學(xué)附屬廣興醫(yī)院;浙江中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金項目(81373757)
【分類號】：R245.81

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