藏藥“蒂達”幾種藥用植物指紋圖譜的建立及其抗肝損傷有效性評價
發(fā)布時間：2018-09-14 10:07
【摘要】：目的建立并比較藏藥“蒂達”11種基原植物的高效液相色譜指紋圖譜,比較藏藥“蒂達”4種基原植物(青葉膽、唐古特虎耳草、印度獐牙菜和川西獐牙菜)體內(nèi)、體外抗肝損傷藥效學(xué)作用,為臨床用藥提供科學(xué)依據(jù)。方法使用HPLC法建立11種“蒂達”基原植物指紋圖譜。“蒂達”4種基原植物對小鼠急性肝損傷保護作用研究中,采用CCl4來建立化學(xué)性肝損傷模型,以青葉膽、唐古特虎耳草、印度獐牙菜和川西獐牙菜兩個劑量(3 g/kg、6 g/kg)灌胃給藥,1次/d,連續(xù)7d,檢測丙氨酸轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)及總蛋白(TP),計算肝臟指數(shù)及觀察肝臟病理組織切片。對小鼠慢性肝損傷保護作用研究中,小鼠皮下注射20%CCl4(2次/周),以青葉膽、唐古特虎耳草、印度獐牙菜和川西獐牙菜兩個劑量(1.5 g/kg、3 g/kg)灌胃給藥。1次/d,連續(xù)45天,檢測丙氨酸轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、總蛋白(TP)及總膽紅素(TBI),計算肝臟指數(shù)、脾臟指數(shù)及胰腺指數(shù)和觀察肝臟病理組織切片。體外抗肝損傷研究中,測定細胞培養(yǎng)上清液中谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力。結(jié)果本課題完成了11種“蒂達”基原植物指紋圖譜共有模式的建立及相似度評價�！暗龠_”四種基原植物對小鼠急性肝損傷保護作用研究中,4種藥物都能夠顯著降低ALT、AST含量(P0.01),川西獐牙菜6 g/kg組效果最好。對小鼠慢性肝損保護作用研究中,4種藥物都能夠顯著降低ALT、AST含量(P0.01),改善肝臟組織病理損傷。川西獐牙菜、印度獐牙菜的保肝效果優(yōu)于青葉膽。體外抗肝損傷研究中,給藥組與模型組比較ALT、AST、MDA含量均下降,SOD活力提升。4種原植物在不同濃度時對不同的酶的作用效果不同,存在一定的差異,綜合結(jié)果顯示:川西獐牙菜、印度獐牙菜的保肝效果優(yōu)于青葉膽。結(jié)論本課題建立了較完整的藏藥“蒂達”的高效液相色譜指紋圖譜,該方法為其質(zhì)量控制及評價提供科學(xué)依據(jù)。青葉膽、唐古特虎耳草、川西獐牙菜和印度獐牙菜均對肝臟有一定保護作用,不同種不同濃度作用于急、慢性CCl4損傷小鼠的抗肝損傷作用存在一定差異,不同種不同濃度對人肝細胞L-O2中不同酶作用效果不同,其機制可能與抗氧化有關(guān)。本課題為指導(dǎo)臨床用藥提供依據(jù)。
[Abstract]:OBJECTIVE To establish and compare the high performance liquid chromatographic fingerprints of 11 primordial plants of the Tibetan medicine Tida, and to compare the pharmacodynamic effects of four primordial plants of the Tibetan medicine Tida in vitro and in vivo, so as to provide scientific basis for clinical use. In the study of the protective effect of the four original plants on acute liver injury in mice, CCl4 was used to establish the model of chemical liver injury. Two doses (3 g/kg, 6 g/kg) were given orally, once a day, for 7 days, to detect alanine aminotransferase. To study the protective effects of 20% CCl4 (twice a week) subcutaneously on chronic liver injury in mice, two doses (1.5 g/kg, 3 g/kg) were administered orally to the mice, i.e. green leaf gall, Tangut tiger's ear grass, Swertia glauca and Swertia roxburghii. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), total protein (TP) and total bilirubin (TBI), liver index, spleen index and pancreatic index were calculated, and liver pathological sections were observed. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), malondialdehyde (MDA) content and malondialdehyde (MDA) content in cell culture supernatant were measured in vitro. Superoxide dismutase (SOD) activity. Results The establishment and similarity evaluation of the fingerprint patterns of 11 Tida primordial plants were completed in this study. Good. In the study of chronic liver injury in mice, the four drugs can significantly reduce the content of ALT, AST (P 0.01), improve liver tissue pathological injury. Swertia, Swertia Swertia, liver protection effect is better than that of Cyanobacteria. In the study of anti-liver injury in vitro, compared with the model group, the content of ALT, AST, MDA decreased, SOD activity increased. The results showed that the liver-protecting effect of Swertia roxburghii and Swertia roxburghii was better than that of Cyanopsis indica. Conclusion A complete high performance liquid chromatography fingerprint of Tida was established, which provided a basis for quality control and evaluation. Evidence. Cyclobalanopsis, Tanggut Saxifraga, Swertia Swertia and Swertia Swertia have protective effects on the liver. Different concentrations have different effects on acute and chronic CCl4-induced liver injury in mice. Different concentrations have different effects on different enzymes in human hepatocyte L-O2. The mechanism may be related to antioxidant effect. This topic provides a basis for guiding clinical medication.
【學(xué)位授予單位】：大理大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R29
[Abstract]:OBJECTIVE To establish and compare the high performance liquid chromatographic fingerprints of 11 primordial plants of the Tibetan medicine Tida, and to compare the pharmacodynamic effects of four primordial plants of the Tibetan medicine Tida in vitro and in vivo, so as to provide scientific basis for clinical use. In the study of the protective effect of the four original plants on acute liver injury in mice, CCl4 was used to establish the model of chemical liver injury. Two doses (3 g/kg, 6 g/kg) were given orally, once a day, for 7 days, to detect alanine aminotransferase. To study the protective effects of 20% CCl4 (twice a week) subcutaneously on chronic liver injury in mice, two doses (1.5 g/kg, 3 g/kg) were administered orally to the mice, i.e. green leaf gall, Tangut tiger's ear grass, Swertia glauca and Swertia roxburghii. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), total protein (TP) and total bilirubin (TBI), liver index, spleen index and pancreatic index were calculated, and liver pathological sections were observed. Alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), malondialdehyde (MDA) content and malondialdehyde (MDA) content in cell culture supernatant were measured in vitro. Superoxide dismutase (SOD) activity. Results The establishment and similarity evaluation of the fingerprint patterns of 11 Tida primordial plants were completed in this study. Good. In the study of chronic liver injury in mice, the four drugs can significantly reduce the content of ALT, AST (P 0.01), improve liver tissue pathological injury. Swertia, Swertia Swertia, liver protection effect is better than that of Cyanobacteria. In the study of anti-liver injury in vitro, compared with the model group, the content of ALT, AST, MDA decreased, SOD activity increased. The results showed that the liver-protecting effect of Swertia roxburghii and Swertia roxburghii was better than that of Cyanopsis indica. Conclusion A complete high performance liquid chromatography fingerprint of Tida was established, which provided a basis for quality control and evaluation. Evidence. Cyclobalanopsis, Tanggut Saxifraga, Swertia Swertia and Swertia Swertia have protective effects on the liver. Different concentrations have different effects on acute and chronic CCl4-induced liver injury in mice. Different concentrations have different effects on different enzymes in human hepatocyte L-O2. The mechanism may be related to antioxidant effect. This topic provides a basis for guiding clinical medication.
【學(xué)位授予單位】：大理大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R29
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