【摘要】：目的:檢測異常黏液質(zhì)型陽痿病證大鼠睪丸組織中ABP、StAr等生精功能相關(guān)關(guān)鍵指標(biāo)和凋亡情況,并結(jié)合睪丸的形態(tài)學(xué)觀察,研究該病證大鼠伴發(fā)少精癥的病理生理學(xué)機制與對證維藥伊木薩克片可能的作用機理。方法:取90只雄性、性功能正常的SD大鼠,隨機取10只為正常對照組(N),余80只為造模組,采用芫荽實+菠菜實+濕寒性環(huán)境的干預(yù)條件建立異常黏液質(zhì)證候模型,動態(tài)觀測大鼠生物學(xué)表征與勃起功能的改變,造模20w后通過APO勃起實驗和性行為學(xué)實驗篩選出陽痿病證模型后,隨機分為病證模型組(A1)及病證藥物反證組(A31),將未成陽痿者隨機分為證候模型組(B1)和證候藥物反證組(B3),反證2w后取大鼠睪丸組織和外周血做一下檢測:(1)用HE染色觀察睪丸組織形態(tài)學(xué)結(jié)構(gòu)變化,用電鏡觀察生精細(xì)胞形態(tài)學(xué)結(jié)構(gòu)變化;(2)用免疫組化法檢測大鼠睪丸組織ABP蛋白和StAr蛋白的變化;(3)用TUNEL法檢測各組大鼠睪丸間質(zhì)細(xì)胞和生精細(xì)胞凋亡情況。結(jié)果:(1)HE染色睪丸生精細(xì)胞形態(tài)學(xué)結(jié)構(gòu)觀察結(jié)果顯示,N組結(jié)構(gòu)完好;B1、A1組管壁上生精細(xì)胞數(shù)量明顯少于N組,間質(zhì)細(xì)胞及精子數(shù)量減少;分別與B1、A1組相比B3、A3組生精小管形態(tài)結(jié)構(gòu)改善,各級生精細(xì)胞數(shù)量有升高趨勢。(2)HE染色結(jié)果顯示:B1、A1組大鼠睪丸間質(zhì)細(xì)胞形態(tài)結(jié)構(gòu)與N組比較呈現(xiàn)不規(guī)則,水腫,顏色較深,細(xì)胞數(shù)量少。B3、A3組睪丸間質(zhì)細(xì)胞數(shù)量與B1、A1組相比有回升趨勢。(3)電鏡結(jié)果顯示:N組睪丸精曲小管結(jié)構(gòu)完整。B1、A1組大鼠睪丸精曲小管形態(tài)結(jié)構(gòu)與N組相比呈現(xiàn)紊亂、各級生精細(xì)胞數(shù)量明顯減少,排列不齊,支持細(xì)胞現(xiàn)粗面內(nèi)質(zhì)網(wǎng)擴(kuò)張現(xiàn)象。B3、A3組大鼠睪丸生精細(xì)胞分界明顯,,但管腔內(nèi)生精細(xì)胞排列數(shù)量少、部分支持細(xì)胞仍然能見到水腫樣改變。(4)TUNEL法檢測睪丸生精細(xì)胞凋亡結(jié)果顯示:與N組相比B1、A1組細(xì)胞凋亡明顯增多(P0.01)。B3、A3組與B1、A1組相比細(xì)胞凋亡顯著下降(P0.01)。(5)TUNEL法檢測睪丸間質(zhì)細(xì)胞凋亡結(jié)果顯示:與N組相比B1、A1組細(xì)胞凋亡明顯增多(P0.01)。B3、A3組與B1、A1組相比細(xì)胞凋亡顯著下降(P0.01)。(6)免疫組化結(jié)果顯示:與N組相比B1、A1組大鼠StAr蛋白表達(dá)明顯下調(diào)。與B1組相比B3組大鼠睪丸StAr蛋白表達(dá)顯著增多(P0.01)。A3組大鼠睪丸StAr蛋白表達(dá)與A1組無顯著差異(P0.05)。(7)免疫組化結(jié)果顯示,B1、A1組大鼠睪丸ABP蛋白表達(dá)下調(diào)(P0.01)。B3、A3組大鼠睪丸ABP蛋白表達(dá)顯著上調(diào)(P0.05)。結(jié)論(1)維醫(yī)異常黏液質(zhì)型陽痿病證大鼠睪丸形態(tài)學(xué)結(jié)構(gòu)發(fā)生改變和生精細(xì)胞的凋亡可能是其發(fā)生少精癥的病理學(xué)基礎(chǔ)。(2)異常黏液質(zhì)型陽痿病證大鼠外周血T水平下降和睪丸組織ABP、StAr蛋白的下調(diào)可能是大鼠出現(xiàn)生精功能改變的重要環(huán)節(jié)之一。(3)伊木薩克片可能通過多個藥物靶點來改善睪丸形態(tài)學(xué)結(jié)構(gòu)而影響異常黏液質(zhì)型陽痿病證大鼠生精功能。
[Abstract]:Objective: to detect the key indexes and apoptosis of spermatogenic function such as ABP,StAr in testis of rats with abnormal mucinous impotence and observe the morphology of testis. To study the pathophysiological mechanism of oligozoospermia and the possible action mechanism of Yimusak tablet. Methods: 90 male SD rats with normal sexual function were randomly selected as control group, and 80 (N), rats as model group. The model of abnormal mucus syndromes was established under the intervention conditions of coriander spinach wet and cold environment. The biological characteristics and erectile function of rats were observed dynamically, and the impotence syndrome model was selected by APO erectile test and sexual behavior experiment 20 weeks later. The patients without impotence were randomly divided into syndrome model group (B1) and syndrome drug negative syndrome group (B3). The testis and peripheral blood of rats were taken for 2 weeks. (1) observe by HE staining. Observing the morphological changes of testis, Morphological changes of spermatogenic cells were observed by electron microscope; (2) changes of ABP and StAr proteins in testis were detected by immunohistochemical method; (3) apoptosis of interstitial cells and spermatogenic cells in testis were detected by TUNEL method. Results: (1) the morphological structure of testicular spermatogenic cells was observed by HE staining. The results showed that the number of spermatogenic cells on the wall of group A 1 was less than that of group N, and the number of interstitial cells and spermatozoa decreased. The morphology and structure of seminiferous tubules were improved and the number of spermatogenic cells increased in group B _ (1) A _ (1) compared with group B _ (1) A _ (1) respectively. (2) the results of HE staining showed that the morphology and structure of interstitial cells of testis in group B _ (1) A _ (1) were irregular, edematous and darker than those in group N. The number of testicular mesenchymal cells in the small number of cells. B _ (3) A _ (3) compared with B _ (1) A _ (1) group, the results of electron microscope showed that the testicular seminiferous tubules in group B _ (1) N had complete structure. The morphological structure of testicular seminiferous tubules in group B _ (1) A _ (1) was disordered compared with that in group N. The number of spermatogenic cells at all levels decreased obviously, and the number of spermatogenic cells in the testis of the Sertoli cells expanded in rough endoplasmic reticulum was obvious, but the number of spermatogenic cells in the lumen was less. Some Sertoli cells can still be seen edematous changes. (4) the results of testicular spermatogenic cell apoptosis detected by TUNEL method: compared with the N group, the apoptosis of B1OA1 group was significantly increased (P0.01). B3A3 group was significantly lower than B1A1 group (P0.01). (5) TUNEL method was used to detect the apoptosis of testicular spermatogenic cells (P0.01). (5). The results of cytoplasmic cell apoptosis showed that the apoptosis of B1A1group was significantly higher than that of group N (P0.01). The expression of StAr protein in B1A1group was significantly decreased compared with that in B1A1group (P0.01). (6). Compared with group B1, the expression of StAr protein in testis of B3 group was significantly increased (P0.01). The expression of StAr protein in testis of group A3 was not significantly different from that of group A1 (P0.05). (7). The results of immunohistochemistry showed that the expression of ABP protein down-regulated (P0.01). B3A3 group significantly up-regulated the expression of ABP protein (P0.05). Conclusion (1) the morphological changes of testis and the apoptosis of spermatogenic cells may be the pathological basis of oligozoospermia in rats with abnormal mucinous impotence syndrome. (2) T level in peripheral blood of rats with abnormal mucinous impotence syndrome. The decrease of ABP,StAr protein and the down-regulation of testicular ABP,StAr protein may be one of the important links of spermatogenic function changes in rats. (3) impotence of abnormal mucinous type may be affected by multiple drug targets to improve the morphological structure of testis. The spermatogenic function of rats with disease syndrome.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R29

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