針刺對(duì)哮喘大鼠肺組織中Eotaxin、RANTES表達(dá)的影響
發(fā)布時(shí)間：2018-08-29 09:28
【摘要】：目的:探討針刺對(duì)支氣管哮喘大鼠肺組織中Eotaxin、RANTES表達(dá)的調(diào)控。方法:將24只SPF級(jí)SD大鼠隨機(jī)分為空白組、哮喘模型組、哮喘模型針刺組,每組8只。各組分別給予相應(yīng)干預(yù)后,采用HE染色法觀察大鼠肺組織中病理變化,免疫熒光染色法檢測(cè)肺組織中Eotaxin、RANTES的表達(dá),ELISA檢測(cè)大鼠BALF及血清中Eotaxin、RANTES表達(dá)的程度。結(jié)果:哮喘模型針刺組大鼠肺組織病理改變較哮喘模型組明顯減輕,與空白組差別不明顯;哮喘模型組Eotaxin、RANTES蛋白表達(dá)高于哮喘模型針刺組(P0.05);哮喘針刺組BALF及血清中Eotaxin、RANTES含量均低于哮喘模型組(P0.05),但與空白組差異不明顯(P0.05)。結(jié)論:哮喘大鼠BALF及血清中Eotaxin、RANTES的表達(dá)水平明顯增高;針刺治療可以明顯降低其表達(dá)水平,提示針刺可以通過調(diào)節(jié)Eotaxin、RANTES的表達(dá)來減輕氣道炎癥反應(yīng)。
[Abstract]:Objective: to investigate the effect of acupuncture on the expression of Eotaxin,RANTES in lung tissues of asthmatic rats. Methods: 24 SPF SD rats were randomly divided into blank group and acupuncture group with 8 rats in each group. The pathological changes of lung tissue were observed by HE staining, the expression of Eotaxin,RANTES in lung tissue was detected by immunofluorescence staining and the expression of BALF and Eotaxin,RANTES in serum were detected by Elisa. Results: the pathological changes of lung tissue in the acupuncture group of asthma model were significantly less than those in the asthmatic model group, but there was no significant difference between the two groups. The expression of Eotaxin,RANTES protein in asthma model group was higher than that in asthma model acupuncture group (P0.05), and the content of BALF and serum Eotaxin,RANTES in asthma acupuncture group was lower than that in asthma model group (P0.05), but there was no significant difference between asthma model group and blank group (P0.05). Conclusion: the expression of Eotaxin,RANTES in BALF and serum of asthmatic rats was significantly increased, and acupuncture treatment could significantly reduce the expression of Eotaxin,RANTES, suggesting that acupuncture can alleviate airway inflammation by regulating the expression of Eotaxin,RANTES.
【作者單位】： 華北理工大學(xué);
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81303046)
【分類號(hào)】：R245
本文編號(hào)：2210793
[Abstract]:Objective: to investigate the effect of acupuncture on the expression of Eotaxin,RANTES in lung tissues of asthmatic rats. Methods: 24 SPF SD rats were randomly divided into blank group and acupuncture group with 8 rats in each group. The pathological changes of lung tissue were observed by HE staining, the expression of Eotaxin,RANTES in lung tissue was detected by immunofluorescence staining and the expression of BALF and Eotaxin,RANTES in serum were detected by Elisa. Results: the pathological changes of lung tissue in the acupuncture group of asthma model were significantly less than those in the asthmatic model group, but there was no significant difference between the two groups. The expression of Eotaxin,RANTES protein in asthma model group was higher than that in asthma model acupuncture group (P0.05), and the content of BALF and serum Eotaxin,RANTES in asthma acupuncture group was lower than that in asthma model group (P0.05), but there was no significant difference between asthma model group and blank group (P0.05). Conclusion: the expression of Eotaxin,RANTES in BALF and serum of asthmatic rats was significantly increased, and acupuncture treatment could significantly reduce the expression of Eotaxin,RANTES, suggesting that acupuncture can alleviate airway inflammation by regulating the expression of Eotaxin,RANTES.
【作者單位】： 華北理工大學(xué);
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81303046)
【分類號(hào)】：R245
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