【摘要】：目的:探討針刺促進(jìn)卵泡生長(zhǎng)、發(fā)育、成熟、排出的效應(yīng)機(jī)理及對(duì)生殖內(nèi)分泌激素的影響,闡釋傳統(tǒng)中醫(yī)療法的科學(xué)內(nèi)涵,為臨床開(kāi)展針刺促排卵提供科學(xué)依據(jù)。方法:將27只具有正常發(fā)情周期的大鼠,按照體重隨機(jī)分成空白組、針刺組、安慰針組3組,每組9只。針刺組選取"中極"、"氣海"、"百會(huì)"、雙側(cè)"三陰交"、雙側(cè)"合谷",電針連接"中極"與"氣海";安慰針組選取未知穴位,雙側(cè)股骨大轉(zhuǎn)子與后海(尾根與肛門(mén)之間的凹陷處)中點(diǎn),雙側(cè)肩峰頂端,淺刺后電針連接雙側(cè)肩峰端,電針設(shè)置為虛擬;空白組大鼠不予干預(yù)。采用陰道涂片觀(guān)察動(dòng)情周期,每天記錄體重并進(jìn)行陰道學(xué)涂片,在動(dòng)情前期第1日開(kāi)始針刺,每日1次,針刺結(jié)束后次日結(jié)合陰道學(xué)涂片,將處于動(dòng)情期大鼠處死,取腹主動(dòng)脈血,檢測(cè)血清雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黃體生成素(LH)、胰島素生長(zhǎng)因子-1(IGF-1)的表達(dá)情況。同時(shí)取大鼠雙側(cè)卵巢組織,稱(chēng)重,并于光學(xué)顯微鏡下觀(guān)察所有視野中的初級(jí)卵泡、次級(jí)卵泡、成熟卵泡的數(shù)目。結(jié)果:①針刺組血清雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黃體生成素(LH)、胰島素生長(zhǎng)因子-1(IGF-1)值與空白組、安慰針組相比,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。②針刺組大鼠卵巢重量及大鼠卵巢指數(shù)與空白組、安慰針組相比,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。③針刺組初級(jí)卵泡數(shù)、次級(jí)卵泡數(shù)及卵泡總數(shù)較空白組、安慰針組多,但p0.05,差異無(wú)統(tǒng)計(jì)學(xué)意義;針刺組與空白組、安慰針組在初級(jí)卵泡率、次級(jí)卵泡率方面相比,差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05);針刺組成熟卵泡率與空白組、安慰針組相比,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論:①針刺可以促進(jìn)卵泡的生長(zhǎng)、發(fā)育,提高成熟卵泡率,促進(jìn)卵泡的排出。②針刺可提高血清中雌二醇(E2)、孕激素(P)、卵泡刺激素(FSH)、黃體生成素(LH)、胰島素生長(zhǎng)因子-1(IGF-1)的水平,從而改善排卵功能,提高受孕率。
[Abstract]:Objective: to explore the effect mechanism of acupuncture on follicle growth, development, maturation and excretion and its effect on reproductive endocrine hormone, to explain the scientific connotation of traditional Chinese medicine therapy, and to provide scientific basis for clinical application of acupuncture to promote ovulation. Methods: 27 rats with normal estrous cycle were randomly divided into three groups according to their body weight: blank group, acupuncture group and comfort needle group, with 9 rats in each group. The acupuncture group selected "middle pole", "air sea", "Baihui", bilateral "Sanyinjiao", bilateral "Hegu", electroacupuncture connecting "middle pole" and "air sea", and consolation needle group selected unknown acupoints, Bilateral trochanter of femur and Houhai (depression between tail root and anus), top of bilateral acromial, electroacupuncture connected to bilateral acromial end after superficial prick, electroacupuncture was set as virtual, blank group rats did not intervene. Vaginal smears were used to observe the estrus cycle, body weight was recorded and vaginal smears were taken daily. Acupuncture was performed on the first day of estrus, once a day, and the rats were killed in estrus the next day after acupuncture combined with vaginal smears. Serum estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor-1 (IGF-1) were collected from abdominal aorta. The number of primary follicles, secondary follicles and mature follicles in all visual fields were observed under optical microscope. Results Serum estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor 1 (IGF-1) were compared with those in control group and comfort needle group. The difference was statistically significant (p0.05) .2 the weight of ovary and the ovarian index of rats in acupuncture group were significantly higher than those in blank group and placebo acupuncture group (p0.05). The number of primary follicles, the number of secondary follicles and the total number of follicles in acupuncture group were significantly higher than those in blank group. There were no significant differences in primary follicle rate and secondary follicle rate between the acupuncture group and the blank group (p0.05), while the mature follicle rate in the acupuncture group was higher than that in the blank group and the placebo needle group (p0.05), while the rate of mature follicles in the acupuncture group was higher than that in the blank group and the placebo needle group (p0.05). The difference was statistically significant (p0.05). Conclusion Acupuncture can promote the growth and development of follicles, increase the rate of mature follicles, promote the excretion of follicles and increase the levels of estradiol (E2) and progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH), insulin growth factor-1 (IGF-1) in serum. Thus, the ovulation function is improved and the pregnancy rate is increased.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R245

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳靜;劉立華;;調(diào)和營(yíng)衛(wèi)外治法治療失眠臨床觀(guān)察[J];世界中西醫(yī)結(jié)合雜志;2017年01期

2 王利紅;徐文婷;;針?biāo)幗Y(jié)合治療未破裂卵泡黃素化綜合征的臨床研究[J];南京中醫(yī)藥大學(xué)學(xué)報(bào);2016年01期

3 閆穎;張晗;吳林玲;金季玲;;金季玲治療青春期多囊卵巢綜合征臨床經(jīng)驗(yàn)[J];山西中醫(yī);2015年02期

4 靳鐳;徐蓓;;黃體生成素在卵泡發(fā)育中的作用及早卵泡期黃體生成素預(yù)處理[J];生殖醫(yī)學(xué)雜志;2014年12期

5 顧W，

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