【摘要】：目的:比較鹽炙前后關(guān)黃柏多糖中總糖、蛋白及糖醛酸含量,考察對小鼠免疫功能的影響,以探討關(guān)黃柏多糖組分的鹽炙機(jī)理。方法:采用苯酚-硫酸法、考馬斯亮藍(lán)法、硫酸-咔唑法分別測定總糖、蛋白及糖醛酸含量,采用經(jīng)典小鼠免疫實(shí)驗(yàn)以檢測鹽炙前后總多糖對小鼠免疫功能的影響。結(jié)果:鹽炙前后關(guān)黃柏多糖總糖、蛋白及糖醛酸含量具有一定差異,關(guān)黃柏多糖中劑量及鹽炙品多糖中、高劑量能降低小鼠碳粒廓清指數(shù);鹽炙關(guān)黃柏多糖中、高劑量對DNFB所致遲發(fā)型超敏反應(yīng)有明顯的抑制作用;鹽炙關(guān)黃柏多糖中、高劑量能明顯降低小鼠溶血素抗體生成能力且呈量效關(guān)系。結(jié)論:關(guān)黃柏經(jīng)鹽炙后多糖類成分發(fā)生明顯變化,且鹽炙前后關(guān)黃柏多糖能從非特異性免疫、細(xì)胞免疫和體液免疫方面抑制小鼠的免疫功能,鹽炙后效果更顯著,說明關(guān)黃柏方法中鹽炙對于發(fā)揮免疫抑制功能有較重要的作用。
[Abstract]:Aim: to compare the contents of total sugar, protein and uronic acid in polysaccharides of Phellodendron officinalis before and after salt-burning, and to investigate the effect on the immune function of mice, and to explore the mechanism of salt-burning of polysaccharides from Phellodendron mandshurica. Methods: the contents of total sugar, protein and uronic acid were determined by phenol-sulfuric acid method, Coomassie brilliant blue method and sulfuric acid-carbazole method respectively. Results: the content of total sugar, protein and uronic acid in polysaccharide of Phellodendron officinalis was different before and after salt treatment. The middle dose and high dose of polysaccharide of Phellodendron mandshurica could reduce the clearance index of carbon particles in mice, and the content of polysaccharide of Phellodendron officinalis in salt-burning was higher than that of control group. The high dose could obviously inhibit the delayed hypersensitivity induced by DNFB, and the high dose could obviously decrease the production ability of hemolysin antibody in mice in a dose-effect manner. Conclusion: the polysaccharide of Phellodendron mandshurica can inhibit the immune function of mice from the aspects of non-specific immunity, cellular immunity and humoral immunity. The results showed that salt-broiling plays an important role in the immunosuppressive function of Phellodendron officinalis.
【作者單位】： 黑龍江中醫(yī)藥大學(xué)教育部北藥基礎(chǔ)與應(yīng)用研究重點(diǎn)實(shí)驗(yàn)室黑龍江省中藥及天然藥物藥效物質(zhì)基礎(chǔ)研究重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81473351) 黑龍江中醫(yī)藥大學(xué)研究生創(chuàng)新科研項(xiàng)目(2015010)
【分類號】：R245
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本文編號：2150080