【摘要】：目的:觀察蟲草保腎顆粒對慢性腎衰竭大鼠腎功能、血脂和同型半胱氨酸水平的影響,探討其能否通過調(diào)控血脂和同型半胱氨酸水平而改善大鼠高脂高凝狀態(tài)。方法:將78只雄性SD大鼠稱重后,隨機分為對照組(26只),模型組(26只),蟲草保腎顆粒組(26只)。三組均給予普通飼料喂養(yǎng),模型組及蟲草組均采用5/6腎切除術(shù)制作CRF模型。給藥方案:造模后,各組開始灌胃給藥,每日一次,持續(xù)12周,蟲草保腎顆粒組給予蟲草保腎顆粒水煎劑灌胃,對照組和模型組給予等量蒸餾水灌胃。分別于造模后0、4、8、12周觀察大鼠一般狀態(tài),并進行24小時尿蛋白定量及血清肌酐(Scr)、尿素氮(BUN)、膽固醇(CHOL)、甘油三酯(TG)、低密度脂蛋白(LDL-C)和同型半胱氨酸(HCy)水平測定,同一時間采樣點每組大鼠處死6只,并采用HE染色法觀察腎組織形態(tài)學(xué)改變。結(jié)果:1.蟲草保腎顆粒能有效改善大鼠一般狀態(tài);2.蟲草保腎顆�？梢越档虲RF大鼠血清Scr、BUN,降低蛋白尿水平,與模型組比較有顯著差異(P0.01,P0.05);3.蟲草保腎顆�？梢越档虲RF大鼠血清CHOL、TG、LDL-C和Hcy水平,與模型組比較有顯著差異(P0.01,P0.05);4.經(jīng)蟲草保腎顆粒治療后,腎小球硬化程度、間質(zhì)纖維化程度、腎小管炎性細胞浸潤程度均有減輕。結(jié)論:1.蟲草保腎顆粒能夠改善CRF大鼠一般狀態(tài),延緩病情進展;2.蟲草保腎顆�？赏ㄟ^降低CRF大鼠血清Scr、BUN水平,減輕蛋白尿,從后延緩病情進展;3.蟲草保腎顆�？梢酝ㄟ^降低血清CHOL、TG、LDL-C和Hcy水平,改善腎衰后期高脂高凝狀態(tài);4.蟲草保腎顆�？梢詼p輕5/6腎切除大鼠腎小球硬化和間質(zhì)纖維化程度。
[Abstract]:Aim: to observe the effects of Cordyceps baoshen granule on renal function, blood lipid and homocysteine levels in rats with chronic renal failure (CRF), and to explore whether Cordyceps baoshen granule can improve hyperlipidemia hypercoagulability by regulating serum lipid and homocysteine levels in rats. Methods: after weighing 78 male SD rats, they were randomly divided into control group (n = 26), model group (n = 26) and cordyceps baoshen granule group (n = 26). The model group and Cordyceps group were treated with 5 / 6 nephrectomy to make CRF model. After the model was made, each group was given intragastric administration once a day for 12 weeks. The control group and the model group were given the same amount of distilled water as the Cordyceps baoshen granule decoction. The general state of rats was observed at 812 weeks after modeling, and the levels of serum creatinine (SCR), urea nitrogen (bun), cholesterol (Chol), triglyceride (TG), low density lipoprotein (LDL-C) and homocysteine (HCy) were measured in 24 hours urine protein quantity and serum levels of creatinine (SCR), urea nitrogen (bun), cholesterol (Chol), low density lipoprotein (LDL-C) and homocysteine (HCy). At the same time, 6 rats in each group were killed and the changes of renal histomorphology were observed by HE staining. The result is 1: 1. Cordyceps baoshen granule can effectively improve the general state of rats. Compared with the model group, Cordyceps baoshen granule could decrease the level of serum Scr-BUNand and proteinuria in CRF rats (P0.01P 0.05). The level of LDL-C and Hcy in serum of CRF rats was significantly lower than that of model group (P0.01P 0.05). After treatment with Cordyceps baoshen granules, the degree of glomerulosclerosis, interstitial fibrosis and infiltration of inflammatory cells in renal tubules were reduced. Conclusion 1. Cordyceps baoshen granule can improve the general state of CRF rats and delay the progression of CRF. Cordyceps baoshen granule can reduce the level of serum Scrn bun and proteinuria in CRF rats, and delay the progression of CRF. Cordyceps sinensis Baoshen granule can improve the hyperlipidemia and hypercoagulability by lowering the serum levels of TGG LDL-C and Hcy in the later stage of renal failure. Cordyceps baoshen granule can reduce the degree of glomerulosclerosis and interstitial fibrosis in 5-6 nephrectomy rats.
【學(xué)位授予單位】：黑龍江省中醫(yī)藥科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R277.5

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