本文選題：蒙古族藥 + 狼毒��； 參考：《中國實驗方劑學(xué)雜志》2017年22期

【摘要】：目的:探討蒙古族藥狼毒炮制減毒藥性理論的科學(xué)性,了解其炮制前后藥效變化,并建立各樣品中巖大戟內(nèi)酯B和狼毒乙素含量測定的RP-HPLC。方法:采用HPLC-Q-TOF/MS技術(shù)比較蒙古族藥狼毒炮制前后的成分差異;通過正常小鼠的急性毒性作用及瀉下作用比較炮制對該藥材毒性和藥效的影響,利用RP-HPLC測定蒙古族藥狼毒及其炮制品中巖大戟內(nèi)酯B和狼毒乙素的含量,流動相乙腈(A)-0.1%冰乙酸水溶液(B)梯度洗脫(0~5 min,40%A;5~10 min,40%~50%A;10~30 min,50%~55%A;30~40 min,55%~60%A;40~45 min,60%~70%A),檢測波長250 nm,柱溫30℃。結(jié)果:與生品相比,蒙古族藥狼毒在炮制后奶制品、訶子湯制品、酒制品中分別有30,20,21個峰的含量降低。狼毒生品及奶、酒、訶子湯制品粉末的半數(shù)致死量分別為2.694,3.642,3.461,4.309 g·kg~(-1)。藥效試驗表明藥物粉末給藥劑量為0.53,0.133 g·kg~(-1)時,狼毒生品及其各炮制品均能明顯提高小鼠小腸墨汁推進率。狼毒中狼毒乙素和巖大戟內(nèi)酯B的線性范圍分別為0.060 12~1.202 4,0.060 72~1.214 4μg,平均加樣回收率分別為107.55%(RSD 4.7%)和103.03%(RSD 3.6%)。結(jié)論:蒙古族藥狼毒不同方法炮制后其化學(xué)成分含量會不同程度降低,進而可能會降低部分刺激性成分含量或使其轉(zhuǎn)化為毒性較小成分,從而降低了其毒性。蒙古族藥狼毒炮制后的粉末較未經(jīng)炮制的粉末毒性降低,炮制后對該藥材的瀉下作用無影響,即炮制可減毒保效。狼毒炮制機制可能與炮制后化學(xué)成分含量變化有關(guān)。建立的HPLC準確可靠、重復(fù)性好,可用于蒙古族藥狼毒及其炮制品中巖大戟內(nèi)B和狼毒乙素的含量測定。
[Abstract]:Objective: To explore the scientificalness of the theory of the drug resistance of Mongolian medicine and to understand the changes of the drug effect before and after its processing, and to establish a RP-HPLC. method for the determination of the content of euphorbiate B and the content of schyomo B in each sample: To compare the difference of the composition of the Mongolian medicine by HPLC-Q-TOF/MS and the acute toxicity of the normal mice. The effects of the artillery on the toxicity and efficacy of the medicinal materials were compared and the content of Euphorbia euphorbialis B and schyomo ethyl in the Mongolian medicine wolf poison and its processed products were measured by RP-HPLC, and the gradient elution of the mobile phase acetonitrile (A) -0.1% glacial acetic acid solution (B) (0~5 min, 40%A; 5~10 min, 40%~50%A; 10~30) 60%~70%A), the detection wavelength was 250 nm, and the column temperature was 30. Results: compared with the raw products, the content of 30,20,21 peaks in the Mongolian medicine wolf poison was lower than that of the milk products, and the half lethal amount of the powder of the raw and milk, wine, and sijo soup products were divided into 2.694,3.642,3.461,4.309 G. Kg~ (-1). When the amount of powder was given to 0.53,0.133 G. Kg~ (-1), the growth rate of murine small intestine ink could be improved obviously. The linear range of 0.060 12~1.202 4,0.060 72~1.214 4 g, respectively, was 107.55% (RSD 4.7%) and 103.03% (RSD 3.6%) respectively. The chemical composition of Mongolian medicine wolf poison can be reduced in varying degrees, and then it may reduce the content of part of the stimulant component or make it into less toxic component, thus reducing its toxicity. The toxicity of the powder produced by Mongolian medicine wolf poison is lower than that of the unprocessed powder. The mechanism of the artillery preparation may be related to the change of chemical composition after the processing. The established HPLC is accurate, reliable and reproducible, and can be used for the determination of the content of B in the Euphorbia Mongolia and its artillery products.
【作者單位】： 江西中醫(yī)藥大學(xué);內(nèi)蒙古民族大學(xué);
【基金】：內(nèi)蒙古自治區(qū)蒙醫(yī)藥科技重大專項(GCY20161219)
【分類號】：R29
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本文編號：2110480