本文選題：腦梗死 + 大鼠��； 參考：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:通過觀察不同頻率電針對腦梗死大鼠腦內(nèi)缺血區(qū)解偶聯(lián)蛋白5(uncoupling protein 5,UCP5)表達(dá)的影響,以探討電針治療腦梗死的中樞機(jī)制。方法:將健康成年雄性SD大鼠60只,隨機(jī)分為空白組、假手術(shù)組、模型組、2Hz電針組、50Hz電針組和100Hz電針組,每組10只。模型組和不同頻率的電針組大鼠應(yīng)用線栓法制備大腦中動脈閉塞腦缺血模型�？瞻捉M、假手術(shù)組及模型組大鼠不予治療,僅進(jìn)行綁縛處理。電針組取大鼠患側(cè)"前三里穴"和"外關(guān)穴",分別給予2Hz、50Hz和100Hz,連續(xù)波電針治療,每次20分鐘,每日1次,連續(xù)治療21天。每組大鼠均在術(shù)后第1、3、7、14、21天,運(yùn)用網(wǎng)屏試驗進(jìn)行行為學(xué)評分,以檢測大鼠的神經(jīng)缺損情況。各組大鼠在第21天對其右側(cè)大腦皮層組織進(jìn)行取材,運(yùn)用免疫組化法檢測大鼠腦缺血區(qū)UCP5陽性表達(dá)的情況。研究結(jié)束后采用SPSS20.0統(tǒng)計軟件對所得數(shù)據(jù)進(jìn)行統(tǒng)計分析。結(jié)果:1.行為學(xué)評分結(jié)果顯示:2Hz組與模型組、50Hz組、100Hz組相比:術(shù)后21天,2Hz組大鼠運(yùn)動神經(jīng)功能優(yōu)于模型組,差異具有統(tǒng)計學(xué)意義(P0.05)。50Hz組與模型組、2Hz組、100Hz組相比:術(shù)后7天,50Hz組與模型組相比,50Hz組大鼠運(yùn)動神經(jīng)功能優(yōu)于模型組,差異有統(tǒng)計學(xué)意義(P0.01);術(shù)后14天,50Hz組與其他各組相比,50Hz組大鼠運(yùn)動神經(jīng)功能均優(yōu)于其他各組,差異存在統(tǒng)計學(xué)意義(P0.05);術(shù)后21天,50Hz組與其他各組相比,50Hz組大鼠運(yùn)動神經(jīng)功能均優(yōu)于其他各組,差異存在統(tǒng)計學(xué)意義(P0.01)。2.大鼠大腦皮層UCP5陽性表達(dá)結(jié)果顯示:空白組與假手術(shù)組、100Hz組比,差異無統(tǒng)計學(xué)意義(P0.05)。模型組與各電針組相比,差異無統(tǒng)計學(xué)意義(P0.05);模型組與空白組相比,模型組大鼠腦保護(hù)作用優(yōu)于空白組,差異具有統(tǒng)計學(xué)意義(P0.01)。不同頻率電針組與其他組相比較:①2Hz組與空白組、50Hz組、100Hz組相比:其中2Hz組與空白組相比,2Hz組大鼠腦保護(hù)作用優(yōu)于空白組,差異有統(tǒng)計學(xué)意義(P0.01);2Hz組與50Hz組相比,差異無統(tǒng)計學(xué)意義(P0.05);2Hz與100Hz組相比,2Hz組大鼠腦保護(hù)作用優(yōu)于100Hz組,差異具有統(tǒng)計學(xué)意義(P0.05)。②50Hz組與空白組、100Hz組相比:其中50Hz組與空白組相比,50Hz組大鼠腦保護(hù)作用優(yōu)于空白組,差異具有統(tǒng)計學(xué)意義(P0.01);50Hz組與100Hz組相比,差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.電針能夠改善腦梗死大鼠運(yùn)動神經(jīng)功能的缺損情況,并促進(jìn)其上肢運(yùn)動功能的恢復(fù)。2.電針治療對于腦梗死具有腦保護(hù)的作用,其機(jī)制可能與其對缺血區(qū)UCP5的上調(diào)有關(guān),不同頻率的電針均能夠上調(diào)UCP5的表達(dá),其中2Hz電針組效果最顯著,其次為50Hz和100Hz電針組。
[Abstract]:Objective: to investigate the central mechanism of electroacupuncture in the treatment of cerebral infarction by observing the effect of different frequency electroacupuncture on the expression of uncoupling protein 5 (UCP5) in ischemic area of rats with cerebral infarction. Methods: sixty healthy adult male SD rats were randomly divided into three groups: blank group, sham operation group, model group, 2 Hz electroacupuncture group and 100 Hz electroacupuncture group, with 10 rats in each group. Middle cerebral artery occlusion (MCAO) model was established in model group and electroacupuncture group with different frequency. Blank group, sham operation group and model group rats were not treated, only tied treatment. In the electroacupuncture group, the "first three li points" and "Waiguan points" in the affected side of rats were treated with 2 Hz and 100 Hz respectively, continuous wave electroacupuncture for 20 minutes, once a day, for 21 days. The rats in each group were assessed with screen test on the first day after operation. The positive expression of UCP5 was detected by immunohistochemical method in the right cerebral cortex of rats on the 21st day. At the end of the study, SPSS 20.0 statistical software was used to analyze the data. The result is 1: 1. The behavioral score showed that the motor nerve function of the rats in the 2 Hz group was better than that in the model group 21 days after operation, compared with that in the 50 Hz group and the 100 Hz group. The difference was statistically significant (P0.05) .50Hz group compared with the model group (2Hz group) and 100Hz group: the motor nerve function of the 50Hz group was superior to that of the model group (P 0.05), and the motor nerve function of the 50Hz group was better than that of the model group. The motor nerve function in 50Hz group was better than that in other groups on the 14th day after operation (P0.01), and the motor nerve function in 50Hz group was better than that in other groups. The difference was statistically significant (P0.05); the motor nerve function in 50Hz group was better than that in other groups 21 days after operation (P0.01). 2. The positive expression of UCP5 in cerebral cortex of rats showed that there was no significant difference between the blank group and the sham operation group (P 0.05). There was no significant difference between the model group and each electroacupuncture group (P0.05); compared with the blank group, the model group had better brain protection than the blank group, the difference was statistically significant (P0.01). The brain protective effect of 2Hz group was better than that of blank group (P0.01), and the difference was statistically significant (P0.01) between 2Hz group and 50Hz group. There was no significant difference (P0.05) the brain protective effect of 2Hz group was better than that of 100Hz group compared with 100Hz group. The difference was statistically significant (P0.05) .250Hz group compared with the blank group (100Hz group): 50Hz group compared with the blank group compared with the control group (50Hz group) was better than the blank group (P < 0.05). The difference was statistically significant (P0.01). There was no significant difference between 50Hz group and 100Hz group (P0.05). Conclusion 1. Electroacupuncture can improve the defect of motor nerve function in rats with cerebral infarction and promote the recovery of motor function of upper limb. Electroacupuncture therapy has the protective effect on cerebral infarction, and its mechanism may be related to the upregulation of UCP5 in ischemic area. Electroacupuncture at different frequencies can up-regulate the expression of UCP5, especially in 2Hz electroacupuncture group, followed by 50Hz and 100Hz electroacupuncture group.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245

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